Reviewed, UniProtKB/Swiss-Prot A7MMC7 (DSBD_ENTS8)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbD
    EC=1.8.1.8
Alternative name(s):
    Protein-disulfide reductase
      Short name=Disulfide reductase

Gene names

Name:	dsbD
Ordered Locus Names:	ESA_00148

Organism

Enterobacter sakazakii (strain ATCC BAA-894) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required to facilitate the formation of correct disulfide bonds in some periplasmic proteins and for the assembly of the periplasmic c-type cytochromes. Acts by transferring electrons from cytoplasmic thioredoxin to the periplasm. This transfer involves a cascade of disulfide bond formation and reduction steps By similarity.
Catalytic activity	Protein dithiol + NAD(P)+ = protein disulfide + NAD(P)H. HAMAP MF_00399
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the thioredoxin family. DsbD subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Electron transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Redox-active center Signal Transmembrane
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro cytochrome complex assembly Inferred from electronic annotation. Source: HAMAP electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: HAMAP protein-disulfide reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 574	555	Thiol:disulfide interchange protein dsbD HAMAP MF_00399		PRO_1000049608
Regions
	Transmembrane	173 – 193	21	Potential
	Transmembrane	218 – 238	21	Potential
	Transmembrane	253 – 273	21	Potential
	Transmembrane	306 – 326	21	Potential
	Transmembrane	333 – 353	21	Potential
	Transmembrane	367 – 387	21	Potential
	Transmembrane	399 – 419	21	Potential
	Domain	430 – 574	145	Thioredoxin
Amino acid modifications
	Disulfide bond	122 ↔ 128		Redox-active By similarity
	Disulfide bond	192 ↔ 314		Redox-active By similarity
	Disulfide bond	489 ↔ 492		Redox-active By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MMC7-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 9063912E6842813A	FASTA	574	61,676
	10 20 30 40 50 60 MAHRILTLIL LFCSAHASAS LFGQQNASQF VPADQAFAFD FQQQQHQLTL NWQIKPGYYL 70 80 90 100 110 120 YRQQIRVTPA NASVAPPALP TGEPHEDEFF GKSEIYRDAL SVPVTVEQAA PGATLSVTYQ 130 140 150 160 170 180 GCAEAGFCYP PETRTVPLSA VEPTESVKAN AATPSAATGE QTRVNSDSPS ATLPFSAFWA 190 200 210 220 230 240 LLIGIGVAFT PCVLPMYPLI SGIVLGGDKR LSTRRALLLA FIYVQGMALT YTALGLVVAA 250 260 270 280 290 300 AGLQFQAALQ SPWVLVTLSA VFVLLALSMF GLFTLQLPAS LQTRLTLMSN RQRGGSPGGV 310 320 330 340 350 360 FAMGAIAGLI CSPCTTAPLS AILLYIAQSG NLWLGGGTLY LYALGMGLPL ILVTVFGNRL 370 380 390 400 410 420 LPKSGPWMEQ VKTAFGFVIL ALPVFLLERV LGEPWGVRLW SVLGVAFFGW AFVTSLNATR 430 440 450 460 470 480 SWMRAVQIVL LGAAMICARP LQDWVFGAPV AESQAHLAFT RIATVDDLDR ALAQAKGKPV 490 500 510 520 530 540 MLDLYADWCV ACKEFEKYTF SAPEVQRALD GAVLLQADVT ANSAADVALL KRLNVLGLPT 550 560 570 IIFFDAQGNE IPNGRVTGFM DAPAFATHLH NRLR

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton B., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000783 Genomic DNA. Translation: ABU75452.1.
RefSeq	YP_001436288.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5549371.
GenomeReviews	Gene locus ESA_00148 in contig CP000783_GR.
KEGG	esa:ESA_00148.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A7MMC7. HTDEYFG.
Family and domain databases
HAMAP	MF_00399. [Tree]
InterPro	IPR003834. Cyt_c_assmbl_TM. IPR017936. Thioredoxin-like. IPR015467. Thioredoxin_core. IPR017937. Thioredoxin_CS. IPR013766. Thioredoxin_domain. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR10438. Trx. 1 hit.
Pfam	PF02683. DsbD. 1 hit. PF00085. Thioredoxin. 1 hit. [Graphical view]
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DSBD_ENTS8

Accession

Primary (citable) accession number: A7MMC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 2, 2007
Last modified:	June 16, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)