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A7MLY2

- TRMB_CROS8

UniProt

A7MLY2 - TRMB_CROS8

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, ESA_00331
Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691S-adenosyl-L-methionine By similarity
Binding sitei94 – 941S-adenosyl-L-methionine By similarity
Binding sitei121 – 1211S-adenosyl-L-methionine By similarity
Active sitei144 – 1441 By similarity
Binding sitei144 – 1441S-adenosyl-L-methionine By similarity
Binding sitei148 – 1481Substrate By similarity
Binding sitei180 – 1801Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciCSAK290339:GJ80-326-MONOMER.
    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferase
    tRNA(m7G46)-methyltransferase
    Gene namesi
    Name:trmB
    Ordered Locus Names:ESA_00331
    OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
    Taxonomic identifieri290339 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
    ProteomesiUP000000260: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 239239tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
    PRO_1000064393Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer By similarity.UniRule annotation

    Protein-protein interaction databases

    STRINGi290339.ESA_00331.

    Structurei

    3D structure databases

    ProteinModelPortaliA7MLY2.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni150 – 1556Interaction with RNA Reviewed prediction
    Regioni217 – 2204Substrate binding By similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0220.
    HOGENOMiHOG000073968.
    KOiK03439.
    OMAiHSTLEMA.
    OrthoDBiEOG6K6VBC.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7MLY2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNDVISPEF DENGRPLRRI RSFVRRQGRL TKGQQHALDH YWPVMGVEYR    50
    AEPLDLVALF GRDAPVTLEI GFGMGSSLVE MAKTNPQQNF LGIEVHSPGV 100
    GACLSAAHEE GVENLRVMCH DAVEVLEKMI PDGSLHMVQL FFPDPWHKAR 150
    HNKRRIVQVP FAQLVLRKLQ PGGVFHMATD WEPYAEHMLE VMSSVEGYEN 200
    LSPTQDYVPR PASRPVTKFE QRGHRLGHGV WDLMFGRVK 239
    Length:239
    Mass (Da):27,184
    Last modified:October 2, 2007 - v1
    Checksum:i5BE484D9CFD39434
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000783 Genomic DNA. Translation: ABU75630.1.
    RefSeqiYP_001436466.1. NC_009778.1.

    Genome annotation databases

    EnsemblBacteriaiABU75630; ABU75630; ESA_00331.
    GeneIDi5549297.
    KEGGiesa:ESA_00331.
    PATRICi20392944. VBICroSak107175_0294.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000783 Genomic DNA. Translation: ABU75630.1 .
    RefSeqi YP_001436466.1. NC_009778.1.

    3D structure databases

    ProteinModelPortali A7MLY2.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 290339.ESA_00331.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABU75630 ; ABU75630 ; ESA_00331 .
    GeneIDi 5549297.
    KEGGi esa:ESA_00331.
    PATRICi 20392944. VBICroSak107175_0294.

    Phylogenomic databases

    eggNOGi COG0220.
    HOGENOMi HOG000073968.
    KOi K03439.
    OMAi HSTLEMA.
    OrthoDBi EOG6K6VBC.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .
    BioCyci CSAK290339:GJ80-326-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
      Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
      PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-894.

    Entry informationi

    Entry nameiTRMB_CROS8
    AccessioniPrimary (citable) accession number: A7MLY2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: October 2, 2007
    Last modified: June 11, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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