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A7MLY2

- TRMB_CROS8

UniProt

A7MLY2 - TRMB_CROS8

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691S-adenosyl-L-methionineUniRule annotation
Binding sitei94 – 941S-adenosyl-L-methionineUniRule annotation
Binding sitei121 – 1211S-adenosyl-L-methionineUniRule annotation
Active sitei144 – 1441By similarity
Binding sitei144 – 1441S-adenosyl-L-methionineUniRule annotation
Binding sitei148 – 1481SubstrateUniRule annotation
Binding sitei180 – 1801SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-326-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:ESA_00331
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
ProteomesiUP000000260: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239tRNA (guanine-N(7)-)-methyltransferasePRO_1000064393Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi290339.ESA_00331.

Structurei

3D structure databases

ProteinModelPortaliA7MLY2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni150 – 1556Interaction with RNAUniRule annotation
Regioni217 – 2204Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073968.
KOiK03439.
OMAiHSTLEMA.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MLY2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNNDVISPEF DENGRPLRRI RSFVRRQGRL TKGQQHALDH YWPVMGVEYR
60 70 80 90 100
AEPLDLVALF GRDAPVTLEI GFGMGSSLVE MAKTNPQQNF LGIEVHSPGV
110 120 130 140 150
GACLSAAHEE GVENLRVMCH DAVEVLEKMI PDGSLHMVQL FFPDPWHKAR
160 170 180 190 200
HNKRRIVQVP FAQLVLRKLQ PGGVFHMATD WEPYAEHMLE VMSSVEGYEN
210 220 230
LSPTQDYVPR PASRPVTKFE QRGHRLGHGV WDLMFGRVK
Length:239
Mass (Da):27,184
Last modified:October 2, 2007 - v1
Checksum:i5BE484D9CFD39434
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU75630.1.
RefSeqiYP_001436466.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU75630; ABU75630; ESA_00331.
GeneIDi5549297.
KEGGiesa:ESA_00331.
PATRICi20392944. VBICroSak107175_0294.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU75630.1 .
RefSeqi YP_001436466.1. NC_009778.1.

3D structure databases

ProteinModelPortali A7MLY2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290339.ESA_00331.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABU75630 ; ABU75630 ; ESA_00331 .
GeneIDi 5549297.
KEGGi esa:ESA_00331.
PATRICi 20392944. VBICroSak107175_0294.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000073968.
KOi K03439.
OMAi HSTLEMA.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci CSAK290339:GJ80-326-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiTRMB_CROS8
AccessioniPrimary (citable) accession number: A7MLY2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 2, 2007
Last modified: October 1, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3