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Protein

Argininosuccinate synthase

Gene

argG

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathway:iL-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argI), Ornithine carbamoyltransferase (argI)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921CitrullineUniRule annotation
Binding sitei97 – 971CitrullineUniRule annotation
Binding sitei122 – 1221ATP; via amide nitrogenUniRule annotation
Binding sitei124 – 1241AspartateUniRule annotation
Binding sitei128 – 1281AspartateUniRule annotation
Binding sitei128 – 1281CitrullineUniRule annotation
Binding sitei129 – 1291AspartateUniRule annotation
Binding sitei132 – 1321CitrullineUniRule annotation
Binding sitei181 – 1811CitrullineUniRule annotation
Binding sitei190 – 1901CitrullineUniRule annotation
Binding sitei266 – 2661CitrullineUniRule annotation
Binding sitei278 – 2781CitrullineUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 209ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-3796-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:ESA_03807
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
ProteomesiUP000000260 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 405405Argininosuccinate synthasePRO_1000000395Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi290339.ESA_03807.

Structurei

3D structure databases

ProteinModelPortaliA7ML85.
SMRiA7ML85. Positions 8-401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7ML85-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNHGIKKVV LAYSGGLDTS AIIPWLKENY EGCDVVACVV DIGQDRDDLK
60 70 80 90 100
GVEQKALRSG ASECYVVDAR EEFIKDYVYP VLQTGALYEG SYLLGTSMAR
110 120 130 140 150
PLIAKALVDV AKKVGADALC HGATGKGNDQ VRFESAWAAL APHLKVIAPW
160 170 180 190 200
REWNLRSREA LLDYLKARDI PTTASLEKIY SRDENAWHIS TEGGVLESPW
210 220 230 240 250
NAPNQDCWVW TVDPKDAPDE AEQVTVTVEK GNVVAVNGEQ LTPFGCLEVL
260 270 280 290 300
NKIGAKHGVG RIDIVENRLV GIKSRGCYET PGGTIMMAAL RGVEQLVLDR
310 320 330 340 350
DSFKWREQVG LEMSYVVYDG RWFAPLRQSL QAAAQSLAEQ VSGEVVLELY
360 370 380 390 400
KGRVTAIQKK STNSLYNEEF ATFGEDEVYD HSHAGGFIRL FSLSSRIRAL

NELKK
Length:405
Mass (Da):44,801
Last modified:October 2, 2007 - v1
Checksum:i25FFA84908623256
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU78993.1.
RefSeqiWP_012126069.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU78993; ABU78993; ESA_03807.
GeneIDi5548188.
KEGGiesa:ESA_03807.
PATRICi20399295. VBICroSak107175_3381.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU78993.1.
RefSeqiWP_012126069.1. NC_009778.1.

3D structure databases

ProteinModelPortaliA7ML85.
SMRiA7ML85. Positions 8-401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290339.ESA_03807.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABU78993; ABU78993; ESA_03807.
GeneIDi5548188.
KEGGiesa:ESA_03807.
PATRICi20399295. VBICroSak107175_3381.

Phylogenomic databases

eggNOGiCOG0137.
HOGENOMiHOG000230093.
KOiK01940.
OMAiIYNGYWW.
OrthoDBiEOG6K9QCV.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciCSAK290339:GJ80-3796-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiASSY_CROS8
AccessioniPrimary (citable) accession number: A7ML85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: July 22, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.