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A7ML10

- DAPE_CROS8

UniProt

A7ML10 - DAPE_CROS8

Protein

Succinyl-diaminopimelate desuccinylase

Gene

dapE

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of N-succinyl-L,L-diaminopimelic acid (SDAP), forming succinate and LL-2,6-diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bacterial cell walls.UniRule annotation

    Catalytic activityi

    N-succinyl-LL-2,6-diaminoheptanedioate + H2O = succinate + LL-2,6-diaminoheptanedioate.UniRule annotation

    Cofactori

    Binds 1 Zn2+ ion per subunit.UniRule annotation
    Binds 1 Co2+ ion per subunit.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi66 – 661Cobalt or zinc 1UniRule annotation
    Active sitei68 – 681UniRule annotation
    Metal bindingi99 – 991Cobalt or zinc 1UniRule annotation
    Metal bindingi99 – 991Cobalt or zinc 2UniRule annotation
    Active sitei133 – 1331Proton acceptorUniRule annotation
    Metal bindingi134 – 1341Cobalt or zinc 2UniRule annotation
    Metal bindingi162 – 1621Cobalt or zinc 1UniRule annotation
    Metal bindingi348 – 3481Cobalt or zinc 2UniRule annotation

    GO - Molecular functioni

    1. cobalt ion binding Source: UniProtKB-HAMAP
    2. metallopeptidase activity Source: InterPro
    3. succinyl-diaminopimelate desuccinylase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. diaminopimelate biosynthetic process Source: UniProtKB-HAMAP
    2. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Amino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis

    Keywords - Ligandi

    Cobalt, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciCSAK290339:GJ80-772-MONOMER.
    UniPathwayiUPA00034; UER00021.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Succinyl-diaminopimelate desuccinylaseUniRule annotation (EC:3.5.1.18UniRule annotation)
    Short name:
    SDAP desuccinylaseUniRule annotation
    Alternative name(s):
    N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolaseUniRule annotation
    Gene namesi
    Name:dapEUniRule annotation
    Ordered Locus Names:ESA_00778
    OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
    Taxonomic identifieri290339 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
    ProteomesiUP000000260: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 375375Succinyl-diaminopimelate desuccinylasePRO_0000375548Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi290339.ESA_00778.

    Structurei

    3D structure databases

    ProteinModelPortaliA7ML10.
    SMRiA7ML10. Positions 4-374.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M20A family. DapE subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0624.
    HOGENOMiHOG000243770.
    KOiK01439.
    OMAiEPMNIGD.
    OrthoDBiEOG60651W.

    Family and domain databases

    Gene3Di3.30.70.360. 1 hit.
    HAMAPiMF_01690. DapE.
    InterProiIPR001261. ArgE/DapE_CS.
    IPR005941. DapE_proteobac.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view]
    PfamiPF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view]
    SUPFAMiSSF55031. SSF55031. 1 hit.
    TIGRFAMsiTIGR01246. dapE_proteo. 1 hit.
    PROSITEiPS00758. ARGE_DAPE_CPG2_1. 1 hit.
    PS00759. ARGE_DAPE_CPG2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7ML10-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSCPVIELTQ QLIRRPSLSP DDAGCQALLI ERLQAVGFTV EPMNIGDTQN    50
    FWAWRGTGET LAFAGHTDVV PAGDVERWIN PPFEPTIRDG MLFGRGAADM 100
    KGSLAAMVVA AERFVAQHPH HRGRLAFLIT SDEEASATNG TVKVVEALMA 150
    RGERLDYCLV GEPSSSEVVG DVVKNGRRGS LTCNLTIHGV QGHVAYPHLA 200
    DNPVHRAAPM LNELVGIEWD RGNEFFPPTS MQIANIQAGT GSNNVIPGDL 250
    HVQFNFRFST ELTDEMIKQR VVALLEKYQL RYTLDWWLSG QPFLTARGKL 300
    VDAVVNAVHH YNEIKPQLLT TGGTSDGRFI ARMGAQVVEL GPVNATIHKI 350
    NECVKASDLQ LLARMYQRIM EQLVA 375
    Length:375
    Mass (Da):41,244
    Last modified:October 2, 2007 - v1
    Checksum:i5F4EB07003167BEF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000783 Genomic DNA. Translation: ABU76055.1.
    RefSeqiYP_001436891.1. NC_009778.1.

    Genome annotation databases

    EnsemblBacteriaiABU76055; ABU76055; ESA_00778.
    GeneIDi5550571.
    KEGGiesa:ESA_00778.
    PATRICi20393765. VBICroSak107175_0690.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000783 Genomic DNA. Translation: ABU76055.1 .
    RefSeqi YP_001436891.1. NC_009778.1.

    3D structure databases

    ProteinModelPortali A7ML10.
    SMRi A7ML10. Positions 4-374.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290339.ESA_00778.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABU76055 ; ABU76055 ; ESA_00778 .
    GeneIDi 5550571.
    KEGGi esa:ESA_00778.
    PATRICi 20393765. VBICroSak107175_0690.

    Phylogenomic databases

    eggNOGi COG0624.
    HOGENOMi HOG000243770.
    KOi K01439.
    OMAi EPMNIGD.
    OrthoDBi EOG60651W.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00021 .
    BioCyci CSAK290339:GJ80-772-MONOMER.

    Family and domain databases

    Gene3Di 3.30.70.360. 1 hit.
    HAMAPi MF_01690. DapE.
    InterProi IPR001261. ArgE/DapE_CS.
    IPR005941. DapE_proteobac.
    IPR002933. Peptidase_M20.
    IPR011650. Peptidase_M20_dimer.
    [Graphical view ]
    Pfami PF07687. M20_dimer. 1 hit.
    PF01546. Peptidase_M20. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55031. SSF55031. 1 hit.
    TIGRFAMsi TIGR01246. dapE_proteo. 1 hit.
    PROSITEi PS00758. ARGE_DAPE_CPG2_1. 1 hit.
    PS00759. ARGE_DAPE_CPG2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
      Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
      PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-894.

    Entry informationi

    Entry nameiDAPE_CROS8
    AccessioniPrimary (citable) accession number: A7ML10
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 26, 2009
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3