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A7MKX5 (HEM6_CROS8) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Coproporphyrinogen-III oxidase, aerobic
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3
Gene names
Name:hemF
Ordered Locus Names:ESA_00813
OrganismCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]
Taxonomic identifier290339 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length301 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333

Catalytic activity

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333

Pathway

Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333

Subunit structure

Homodimer By similarity. HAMAP MF_00333

Subcellular location

Cytoplasm By similarity HAMAP MF_00333.

Sequence similarities

Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   Cellular componentCytoplasm
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processporphyrin-containing compound biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncoproporphyrinogen oxidase activity

Inferred from electronic annotation. Source: EC

protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 301301Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333
PRO_1000019470

Regions

Region48 – 5710Important for dimerization By similarity
Region108 – 1103Substrate binding By similarity
Region240 – 27536Important for dimerization By similarity
Region258 – 2636Substrate binding By similarity

Sites

Active site1061Proton donor By similarity
Binding site921Substrate By similarity
Site1751Important for dimerization By similarity

Sequences

Sequence LengthMass (Da)Tools
A7MKX5 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 63637B081A23FA1B

FASTA30134,581
        10         20         30         40         50         60 
MAPDIQRVKA FLLDLQDRIC QQLSAVDGDD FVEDAWQREG GGGGRSRVLR DGVVFEQAGV 

        70         80         90        100        110        120 
NFSHVHGDAM PASATAHRPE LAGRSFEAMG VSLVVHPRNP YVPTSHANVR FFIAEKPGAD 

       130        140        150        160        170        180 
PVWWFGGGFD LTPFYGFEED AVHWHRTARD LCAPFGDDVY PRYKKWCDDY FYLKHRQEAR 

       190        200        210        220        230        240 
GIGGLFFDDL NTPDFDTCFA FMQAVGDGYL NAYLPIVERR KTLPWGERER EFQLYRRGRY 

       250        260        270        280        290        300 
VEFNLVWDRG TLFGLQTGGR TESILMSMPP LVRWEYGYQP EENSPEAALY RDFLPVRDWV 


K

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References

[1]"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
PLoS ONE 5:E9556-E9556(2010) [PubMed: 20221447] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-894.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000783 Genomic DNA. Translation: ABU76090.1.
RefSeqYP_001436926.1. NC_009778.1.

3D structure databases

ProteinModelPortalA7MKX5.
SMRA7MKX5. Positions 1-300.
ModBaseSearch...

Protein-protein interaction databases

STRINGA7MKX5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5550438.
GenomeReviewsGene locus ESA_00813 in contig CP000783_GR.
KEGGesa:ESA_00813.
PATRIC20393831. VBICroSak107175_0723.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0408.
HOGENOMHBG631180.
OMAVKAYLLD.
ProtClustDBPRK05330.

Family and domain databases

HAMAPMF_00333. Coprogen_oxidas.
[Tree]
InterProIPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
[Graphical view]
Gene3DG3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
KOK00228.
PANTHERPTHR10755. Coprogen_oxidas. 1 hit.
PfamPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSPR00073. COPRGNOXDASE.
SUPFAMSSF102886. Coprogen_oxidas. 1 hit.
PROSITEPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM6_CROS8
AccessionPrimary (citable) accession number: A7MKX5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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