A7MKR1 (GHRB_CROS8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyoxylate/hydroxypyruvate reductase B
EC=1.1.1.79
EC=1.1.1.81

Gene names

Name:	ghrB
Ordered Locus Names:	ESA_04175

Organism

Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	324 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP MF_01667
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01667 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01667
Subunit structure	Homodimer By similarity. HAMAP MF_01667
Subcellular location	Cytoplasm By similarity HAMAP MF_01667.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: EC hydroxypyruvate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 324	324	Glyoxylate/hydroxypyruvate reductase B HAMAP MF_01667		PRO_0000348380
Sites
	Active site	237	1	By similarity
	Active site	266	1	By similarity
	Active site	285	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MKR1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 2F02C5A38C0F909B	FASTA	324	35,164
	10 20 30 40 50 60 MKPSVILYKS LPDDLLARLE SHFNVTRVPD LSPETIEAHA SAFSEAQGLL GSSEKVDAAL 70 80 90 100 110 120 LEKMPALRAA STVSVGYDNF DVDALSAKKI ALMHTPTVLT ETVADTLMTL VLTTARRALE 130 140 150 160 170 180 VAERVKAGEW TGSIGPDWFG CDVHHKTLGI VGMGRIGLAL AQRAHFGFNM PILYNARRHH 190 200 210 220 230 240 SEAEERFNAR YCDLDTLLAE SDFVCVILPL TDETHHMIGA EQFRKMKKSA IFINAGRGPV 250 260 270 280 290 300 VDENALIAAL QSGEIHAAGL DVFEQEPLSK DSPLLTMKNV VALPHIGSAT HETRYNMAAC 310 320 AVDNLINALN GDVSQNCVNP KAVK

References

[1]

"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species." Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J. PLoS ONE 5:E9556-E9556(2010) [PubMed: 20221447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-894.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000783 Genomic DNA. Translation: ABU79356.1.
RefSeq	YP_001440192.1. NC_009778.1.
3D structure databases
ProteinModelPortal	A7MKR1.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MKR1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5550656.
GenomeReviews	Gene locus ESA_04175 in contig CP000783_GR.
KEGG	esa:ESA_04175.
PATRIC	20399967. VBICroSak107175_3712.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1052.
HOGENOM	HBG731446.
OMA	HALTEKK.
ProtClustDB	PRK15409.
Family and domain databases
HAMAP	MF_01667. 2-Hacid_dh_C_GhrB. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR023756. Glyo/OHPyrv_Rdtase_B. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KO	K00090.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. 1 hit. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRB_CROS8

Accession

Primary (citable) accession number: A7MKR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program