A7MKK9 (CYSG2_CROS8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Siroheme synthase 2

Including the following 3 domains:

1. Uroporphyrinogen-III C-methyltransferase
   Short name=Urogen III methylase
   EC=2.1.1.107
   Alternative name(s):
   SUMT
   Uroporphyrinogen III methylase
   Short name=UROM
2. Precorrin-2 dehydrogenase
   EC=1.3.1.76
3. Sirohydrochlorin ferrochelatase
   EC=4.99.1.4

Gene names

Name:	cysG2
Ordered Locus Names:	ESA_04366

Organism

Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	457 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into siroheme. This reaction consists of the NAD-dependent oxidation of precorrin-2 into sirohydrochlorin and its subsequent ferrochelation into siroheme By similarity. HAMAP MF_01646
Catalytic activity	S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1. HAMAP MF_01646 S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2. HAMAP MF_01646 Precorrin-2 + NAD+ = sirohydrochlorin + NADH. HAMAP MF_01646 Siroheme + 2 H+ = sirohydrochlorin + Fe2+. HAMAP MF_01646
Pathway	Cofactor biosynthesis; adenosylcobalamin biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646 Cofactor biosynthesis; adenosylcobalamin biosynthesis; sirohydrochlorin from precorrin-2: step 1/1. Porphyrin metabolism; siroheme biosynthesis; precorrin-2 from uroporphyrinogen III: step 1/1. HAMAP MF_01646 Porphyrin metabolism; siroheme biosynthesis; siroheme from sirohydrochlorin: step 1/1. HAMAP MF_01646 Porphyrin metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.
Sequence similarities	Belongs to the precorrin methyltransferase family.

Ontologies

Keywords
Biological process	Cobalamin biosynthesis Porphyrin biosynthesis
Ligand	NAD S-adenosyl-L-methionine
Molecular function	Lyase Methyltransferase Oxidoreductase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	cobalamin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW siroheme biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro precorrin-2 dehydrogenase activity Inferred from electronic annotation. Source: EC sirohydrochlorin ferrochelatase activity Inferred from electronic annotation. Source: EC uroporphyrin-III C-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 457	457	Siroheme synthase 2 HAMAP MF_01646		PRO_0000330503
Regions
	Region	218 – 457	240	Uroporphyrinogen-III C-methyltransferase HAMAP MF_01646

Sequences

	Sequence		Length	Mass (Da)
	A7MKK9 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 612890921BD2F761	FASTA	457	50,052
	10 20 30 40 50 60 MDHLPIFCQL RHRACLLVGG GDVAERKARL LLEAGAALTV NALAFAPQFK AWAKQGMLRL 70 80 90 100 110 120 VQGEFNASLL DDCWLAIAAT DDDAVNNQVS EAAEARRIFC NVVDAPKQAS FIMPSIIDRS 130 140 150 160 170 180 PLMVAISSGG TSPVLARLLR EKLEALLPQH LGKVAGYAGQ LRRRVKQTFT SMSERRRFWE 190 200 210 220 230 240 KFFVNDRLAQ SLANDDEQAV TRITETLLSE PLDDRGEVVL VGAGPGDPGL LTLKGLQQIQ 250 260 270 280 290 300 QADIVVYDRL VSDEIMNLVR RDADRVFVGK RAGYHCVPQE EINQILLREA QRGKRVVRLK 310 320 330 340 350 360 GGDPFIFGRG GEELETLCDA GIPFSVVPGI TAASGCSAYA GLPLTHRDYA QSVRLITGHL 370 380 390 400 410 420 KNGGEFDWHN LAAEKQTLVF YMGLNQAAAI QEKLIEHGMD PQMPVALVEN GTSVKQRVVA 430 440 450 GVLTELGALA QRVESPSLII VGRVVALRDK LNWFSSK

References

[1]

"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species." Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J. PLoS ONE 5:E9556-E9556(2010) [PubMed: 20221447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-894.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000783 Genomic DNA. Translation: ABU79545.1.
RefSeq	YP_001440381.1. NC_009778.1.
3D structure databases
ProteinModelPortal	A7MKK9.
SMR	A7MKK9. Positions 1-456.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MKK9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5548171.
GenomeReviews	Gene locus ESA_04366 in contig CP000783_GR.
KEGG	esa:ESA_04366.
PATRIC	20400327. VBICroSak107175_3891.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0007.
HOGENOM	HBG730212.
OMA	MCRRDAE.
ProtClustDB	PRK10637.
Family and domain databases
HAMAP	MF_01646. Siroheme_synth. [Tree]
InterPro	IPR000878. 4pyrrol_Mease. IPR014777. 4pyrrole_Mease_sub1. IPR014776. 4pyrrole_Mease_sub2. IPR006366. CobA_cysG_C. IPR016040. NAD(P)-bd_dom. IPR012409. Sirohaem_synth. IPR019478. Sirohaem_synthase_dimer_dom. IPR006367. Sirohaem_synthase_N. IPR003043. Uropor_MeTrfase_CS. [Graphical view]
Gene3D	G3DSA:3.40.1010.10. 4pyrrole_Mease_sub1. 1 hit. G3DSA:3.30.950.10. 4pyrrole_Mease_sub2. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.8.210. Sirohaem_synthase_dimer_dom. 1 hit.
KO	K02302.
Pfam	PF10414. CysG_dimeriser. 1 hit. PF00590. TP_methylase. 1 hit. [Graphical view]
PIRSF	PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAM	SSF53790. Cor/por_Metransf. 1 hit.
TIGRFAMs	TIGR01469. CobA_cysG_Cterm. 1 hit. TIGR01470. CysG_Nterm. 1 hit.
PROSITE	PS00839. SUMT_1. 1 hit. PS00840. SUMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSG2_CROS8

Accession

Primary (citable) accession number: A7MKK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program