Reviewed, UniProtKB/Swiss-Prot A7MKD3 (DADA_ENTS8)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	ESA_01469

Organism

Enterobacter sakazakii (strain ATCC BAA-894) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity.
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity.
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000066094
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	A7MKD3-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 196877CD56892737	FASTA	432	47,402
	10 20 30 40 50 60 MHVVVLGSGV VGVASAWYLR QAGHEVTVID REPGPALETS AANAGQISPG YAAPWAAPGV 70 80 90 100 110 120 PLKAIKWMFQ RHAPLAISLD GTHFQLKWMW QMLRNCDTRH YMQNKGRMVR LAEYSRDCLK 130 140 150 160 170 180 ALRESTGIQY EGRQGGTLQL FRTEQQYENA SRDIAVLESE GVPFRLLKAA ELAQVEPALA 190 200 210 220 230 240 DVAHKLTGGL QLPNDETGDC QLFTTRLAQM AEAAGVVFRY NTPVDRLLSE GQRIYGVKCG 250 260 270 280 290 300 DEVIKADGYV MAFGSYSTAM LKGIVDIPVY PLKGYSLTIP IADESGAPVS TVLDETYKIA 310 320 330 340 350 360 ITRFDNRIRV GGMAEIVGFN TELLKPRQET LEMVVRDLYP RGGHIEQAKF WTGLRPMTPD 370 380 390 400 410 420 GTPVVGRTEF KNLWLNTGHG TLGWTMACGS GQLLSDIISG RTPAIPFEDL SVARYAPGFT 430 PSSSHRLHGA HP

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton B., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000783 Genomic DNA. Translation: ABU76727.1.
RefSeq	YP_001437563.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5552319.
GenomeReviews	Gene locus ESA_01469 in contig CP000783_GR.
KEGG	esa:ESA_01469.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A7MKD3. MFQKHAP.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_ENTS8

Accession

Primary (citable) accession number: A7MKD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 2, 2007
Last modified:	June 16, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)