A7MKD3 (DADA_CROS8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-amino acid dehydrogenase small subunit
EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	ESA_01469

Organism

Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity. HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity. HAMAP MF_01202
Pathway	Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the DadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_1000066094
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	A7MKD3 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 196877CD56892737	FASTA	432	47,402
	10 20 30 40 50 60 MHVVVLGSGV VGVASAWYLR QAGHEVTVID REPGPALETS AANAGQISPG YAAPWAAPGV 70 80 90 100 110 120 PLKAIKWMFQ RHAPLAISLD GTHFQLKWMW QMLRNCDTRH YMQNKGRMVR LAEYSRDCLK 130 140 150 160 170 180 ALRESTGIQY EGRQGGTLQL FRTEQQYENA SRDIAVLESE GVPFRLLKAA ELAQVEPALA 190 200 210 220 230 240 DVAHKLTGGL QLPNDETGDC QLFTTRLAQM AEAAGVVFRY NTPVDRLLSE GQRIYGVKCG 250 260 270 280 290 300 DEVIKADGYV MAFGSYSTAM LKGIVDIPVY PLKGYSLTIP IADESGAPVS TVLDETYKIA 310 320 330 340 350 360 ITRFDNRIRV GGMAEIVGFN TELLKPRQET LEMVVRDLYP RGGHIEQAKF WTGLRPMTPD 370 380 390 400 410 420 GTPVVGRTEF KNLWLNTGHG TLGWTMACGS GQLLSDIISG RTPAIPFEDL SVARYAPGFT 430 PSSSHRLHGA HP

References

[1]

"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species." Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J. PLoS ONE 5:E9556-E9556(2010) [PubMed: 20221447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-894.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000783 Genomic DNA. Translation: ABU76727.1.
RefSeq	YP_001437563.1. NC_009778.1.
3D structure databases
ProteinModelPortal	A7MKD3.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MKD3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5552319.
GenomeReviews	Gene locus ESA_01469 in contig CP000783_GR.
KEGG	esa:ESA_01469.
PATRIC	20395021. VBICroSak107175_1302.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0665.
HOGENOM	HBG729204.
OMA	VDRQPAV.
ProtClustDB	PRK00711.
Family and domain databases
HAMAP	MF_01202. DadA. [Tree]
InterPro	IPR023080. D-aa_DH_ssu_DadA. IPR006076. FAD-dep_OxRdtase. [Graphical view]
KO	K00285.
Pfam	PF01266. DAO. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DADA_CROS8

Accession

Primary (citable) accession number: A7MKD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program