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Protein

Sulfate adenylyltransferase subunit 1

Gene

cysN

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

May be the GTPase, regulating ATP sulfurylase activity.UniRule annotation

Catalytic activityi

ATP + sulfate = diphosphate + adenylyl sulfate.UniRule annotation

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes sulfite from sulfate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi34 – 418GTPUniRule annotation
Nucleotide bindingi113 – 1175GTPUniRule annotation
Nucleotide bindingi168 – 1714GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

ATP-binding, GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-535-MONOMER.
UniPathwayiUPA00140; UER00204.

Names & Taxonomyi

Protein namesi
Recommended name:
Sulfate adenylyltransferase subunit 1UniRule annotation (EC:2.7.7.4UniRule annotation)
Alternative name(s):
ATP-sulfurylase large subunitUniRule annotation
Sulfate adenylate transferaseUniRule annotation
Short name:
SATUniRule annotation
Gene namesi
Name:cysNUniRule annotation
Ordered Locus Names:ESA_00540
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
Proteomesi
  • UP000000260 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Sulfate adenylyltransferase subunit 1PRO_1000008905Add
BLAST

Interactioni

Subunit structurei

Heterodimer composed of CysD, the smaller subunit, and CysN.UniRule annotation

Protein-protein interaction databases

STRINGi290339.ESA_00540.

Structurei

3D structure databases

ProteinModelPortaliA7MJ69.
SMRiA7MJ69. Positions 20-435.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 241217tr-type GAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni34 – 418G1By similarity
Regioni92 – 965G2By similarity
Regioni113 – 1164G3By similarity
Regioni168 – 1714G4By similarity
Regioni206 – 2083G5By similarity

Sequence similaritiesi

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. CysN/NodQ subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG2895. LUCA.
HOGENOMiHOG000229289.
KOiK00956.
OMAiIQDVYKF.
OrthoDBiEOG6GR38Q.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00062. Sulf_adenylyltr_sub1.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MJ69-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTTIAQQIA KEGGVEAYLH AQQHKSLLRF LTCGSVDDGK STLIGRLLHD
60 70 80 90 100
TRQIYEDQLS SLHNDSKRHG TQGEKLDLAL LVDGLQAERE QGITIDVAYR
110 120 130 140 150
YFSTEKRKFI IADTPGHEQY TRNMATGAST CDLAILLMDA RKGVLDQTRR
160 170 180 190 200
HSFISTLLGI KHLVVAVNKM DLVDFSEETF ERIRQDYLTF AGQLPGNLDI
210 220 230 240 250
RFVPLSALEG DNVAVQSQNM PWYTGPTLLE VLENIEIQRV VDEQPLRFPV
260 270 280 290 300
QYVNRPNLDF RGYAGTVAGG VVKVGQRVKA LPSGVESSVA RIVTFDGDLE
310 320 330 340 350
EAGAGEAVTL VLKDEIDISR GDLLVDASQT LAAVQSAAVD VVWMAEQPLV
360 370 380 390 400
PGQSYDIKIA GKKTRARVDN IHYQVDINNL TQRVVENLPL NGIGLVDLTF
410 420 430 440 450
DEPLNLDKYQ ENPVTGGLIF IDRLSNVTVG AGMVREPQQN VYQEPSAFSA
460 470
FELELNQLIR RHFPHWGARD LLGGK
Length:475
Mass (Da):52,661
Last modified:October 2, 2007 - v1
Checksum:iC4D242D28CF7CADB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU75831.1.
RefSeqiWP_007777742.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU75831; ABU75831; ESA_00540.
GeneIDi5551791.
KEGGiesa:ESA_00540.
PATRICi20393332. VBICroSak107175_0484.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU75831.1.
RefSeqiWP_007777742.1. NC_009778.1.

3D structure databases

ProteinModelPortaliA7MJ69.
SMRiA7MJ69. Positions 20-435.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290339.ESA_00540.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABU75831; ABU75831; ESA_00540.
GeneIDi5551791.
KEGGiesa:ESA_00540.
PATRICi20393332. VBICroSak107175_0484.

Phylogenomic databases

eggNOGiENOG4105C3T. Bacteria.
COG2895. LUCA.
HOGENOMiHOG000229289.
KOiK00956.
OMAiIQDVYKF.
OrthoDBiEOG6GR38Q.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00204.
BioCyciCSAK290339:GJ80-535-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00062. Sulf_adenylyltr_sub1.
InterProiIPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR011779. SO4_adenylTrfase_lsu.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR009001. Transl_elong_EF1A/Init_IF2_C.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF50465. SSF50465. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR02034. CysN. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiCYSN_CROS8
AccessioniPrimary (citable) accession number: A7MJ69
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: November 11, 2015
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.