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A7MJ67

- CYSG1_CROS8

UniProt

A7MJ67 - CYSG1_CROS8

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Protein
Siroheme synthase 1
Gene
cysG1, ESA_00538
Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Multifunctional enzyme that catalyzes the SAM-dependent methylations of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 via precorrin-1. Then it catalyzes the NAD-dependent ring dehydrogenation of precorrin-2 to yield sirohydrochlorin. Finally, it catalyzes the ferrochelation of sirohydrochlorin to yield siroheme By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.UniRule annotation
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.UniRule annotation
Precorrin-2 + NAD+ = sirohydrochlorin + NADH.UniRule annotation
Siroheme + 2 H+ = sirohydrochlorin + Fe2+.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei224 – 2241S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Active sitei247 – 2471Proton acceptor By similarity
Active sitei269 – 2691Proton donor By similarity
Binding sitei305 – 3051S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei382 – 3821S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding sitei411 – 4111S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 232NAD By similarity
Nucleotide bindingi43 – 442NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. precorrin-2 dehydrogenase activity Source: UniProtKB-HAMAP
  3. sirohydrochlorin ferrochelatase activity Source: UniProtKB-EC
  4. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-HAMAP
  2. siroheme biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

NAD, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-533-MONOMER.
UniPathwayiUPA00148; UER00211.
UPA00148; UER00222.
UPA00262; UER00211.
UPA00262; UER00222.
UPA00262; UER00376.

Names & Taxonomyi

Protein namesi
Recommended name:
Siroheme synthase 1
Including the following 3 domains:
Uroporphyrinogen-III C-methyltransferase 1 (EC:2.1.1.107)
Short name:
Urogen III methylase 1
Alternative name(s):
SUMT 1
Uroporphyrinogen III methylase 1
Short name:
UROM 1
Precorrin-2 dehydrogenase 1 (EC:1.3.1.76)
Sirohydrochlorin ferrochelatase 1 (EC:4.99.1.4)
Gene namesi
Name:cysG1
Ordered Locus Names:ESA_00538
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
ProteomesiUP000000260: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471Siroheme synthase 1UniRule annotation
PRO_0000330502Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiA7MJ67.

Interactioni

Protein-protein interaction databases

STRINGi290339.ESA_00538.

Structurei

3D structure databases

ProteinModelPortaliA7MJ67.
SMRiA7MJ67. Positions 1-456.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 203203precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase By similarity
Add
BLAST
Regioni215 – 471257Uroporphyrinogen-III C-methyltransferase By similarity
Add
BLAST
Regioni300 – 3023S-adenosyl-L-methionine binding By similarity
Regioni330 – 3312S-adenosyl-L-methionine binding By similarity

Sequence similaritiesi

In the C-terminal section; belongs to the precorrin methyltransferase family.

Phylogenomic databases

eggNOGiCOG0007.
HOGENOMiHOG000290518.
KOiK02302.
OMAiLHQQLAW.
OrthoDBiEOG6DRPFR.

Family and domain databases

Gene3Di1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPiMF_01646. Siroheme_synth.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view]
PIRSFiPIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MJ67-1 [UniParc]FASTAAdd to Basket

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MEYLPLFAQL KQRPVLVVGG GEVALRKIAL LRRAGACVSV VAKKLHPELA    50
ALEQEGALRW LAQSFEPAQL DAVFLVIAAT SDAALNRRVF DEANARQRLV 100
NVVDDQPLCS FIFPSIVDRS PLIVAISSSG NAPVLARLLR EKIESLLPAS 150
LGRMAEVAGG FRDRIKARIP TTDGRRRFWE KAFRGRFASL MAAGDTRAAE 200
AVLEAELDAP QGPQGEIILV GAGPGDAGLL TLRGLQVMQQ ADVVLYDHLV 250
SDGVLDLVRR DADRICVGKR AGAHAVAQHE TNQMLIDFAR EGKTVVRLKG 300
GDPFIFGRGG EELEAAKAAG VPFQVVPGVT AASGATAYAG IPLTHRDYAQ 350
SAVFVTGHYK PDSEPFDWAL LAKSRQTLAI YMGTMKAAEI SQQLIAHGRD 400
ATTPVAVISR GTREDQRVLT GTLDTLNILA KDAPMPALLV VGEVVQLHQQ 450
LAWFQHSTDA ENLRSSVVNL A 471
Length:471
Mass (Da):50,673
Last modified:October 2, 2007 - v1
Checksum:i28F682F12075C41B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000783 Genomic DNA. Translation: ABU75829.1.
RefSeqiWP_012123930.1. NC_009778.1.
YP_001436665.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU75829; ABU75829; ESA_00538.
GeneIDi5551809.
KEGGiesa:ESA_00538.
PATRICi20393328. VBICroSak107175_0482.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000783 Genomic DNA. Translation: ABU75829.1 .
RefSeqi WP_012123930.1. NC_009778.1.
YP_001436665.1. NC_009778.1.

3D structure databases

ProteinModelPortali A7MJ67.
SMRi A7MJ67. Positions 1-456.
ModBasei Search...

Protein-protein interaction databases

STRINGi 290339.ESA_00538.

Proteomic databases

PRIDEi A7MJ67.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABU75829 ; ABU75829 ; ESA_00538 .
GeneIDi 5551809.
KEGGi esa:ESA_00538.
PATRICi 20393328. VBICroSak107175_0482.

Phylogenomic databases

eggNOGi COG0007.
HOGENOMi HOG000290518.
KOi K02302.
OMAi LHQQLAW.
OrthoDBi EOG6DRPFR.

Enzyme and pathway databases

UniPathwayi UPA00148 ; UER00211 .
UPA00148 ; UER00222 .
UPA00262 ; UER00211 .
UPA00262 ; UER00222 .
UPA00262 ; UER00376 .
BioCyci CSAK290339:GJ80-533-MONOMER.

Family and domain databases

Gene3Di 1.10.8.210. 1 hit.
3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
3.40.50.720. 1 hit.
HAMAPi MF_01646. Siroheme_synth.
InterProi IPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR016040. NAD(P)-bd_dom.
IPR012409. Sirohaem_synth.
IPR019478. Sirohaem_synthase_dimer_dom.
IPR006367. Sirohaem_synthase_N.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view ]
Pfami PF10414. CysG_dimeriser. 1 hit.
PF13241. NAD_binding_7. 1 hit.
PF00590. TP_methylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF036426. Sirohaem_synth. 1 hit.
SUPFAMi SSF53790. SSF53790. 1 hit.
TIGRFAMsi TIGR01469. cobA_cysG_Cterm. 1 hit.
TIGR01470. cysG_Nterm. 1 hit.
PROSITEi PS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiCYSG1_CROS8
AccessioniPrimary (citable) accession number: A7MJ67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 2, 2007
Last modified: September 3, 2014
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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