A7MJ65 (CYSH_CROS8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoadenosine phosphosulfate reductase
EC=1.8.4.8

Alternative name(s):
3'-phosphoadenylylsulfate reductase
PAPS reductase, thioredoxin dependent
PAPS sulfotransferase
PAdoPS reductase

Gene names

Name:	cysH
Ordered Locus Names:	ESA_00536

Organism

Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity HAMAP MF_00063.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_1000008924

Sequences

	Sequence		Length	Mass (Da)
	A7MJ65 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 87447AD9F09E55AA	FASTA	244	27,788
	10 20 30 40 50 60 MSVLDLHALN ALEKDARAGA LADTNAQLEA LSAEARVSWA LENLPGEFVL SSSFGIQAAV 70 80 90 100 110 120 CLHLVTQQRP DIPVILTDTG YLFPETYRFI DELTEKLNLN LKIYRAEQSP AWQEARYGKL 130 140 150 160 170 180 WEQGVEGIEK YNDINKVEPM NRALKELNAQ TWFAGLRRDQ SGSRANLPVL GVQRGVFKIL 190 200 210 220 230 240 PIIDWDNRTV YQYLQKHGLK YHPLWDEGYL SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG

References

[1]

"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species." Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J. PLoS ONE 5:E9556-E9556(2010) [PubMed: 20221447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-894.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000783 Genomic DNA. Translation: ABU75827.1.
RefSeq	YP_001436663.1. NC_009778.1.
3D structure databases
ProteinModelPortal	A7MJ65.
SMR	A7MJ65. Positions 2-244.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MJ65.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5551840.
GenomeReviews	Gene locus ESA_00536 in contig CP000783_GR.
KEGG	esa:ESA_00536.
PATRIC	20393324. VBICroSak107175_0480.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0175.
HOGENOM	HBG758022.
OMA	AIHGTRF.
ProtClustDB	PRK02090.
Family and domain databases
HAMAP	MF_00063. CysH. [Tree]
InterPro	IPR004511. PAPS/APS_Rdtase. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase_CysH. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K00390.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. CysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_CROS8

Accession

Primary (citable) accession number: A7MJ65

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program