Reviewed, UniProtKB/Swiss-Prot A7MJ65 (CYSH_ENTS8)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	ESA_00536

Organism

Enterobacter sakazakii (strain ATCC BAA-894) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	244 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite.
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin.
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 244	244	Phosphoadenosine phosphosulfate reductase		PRO_1000008924

Sequences

	Sequence		Length	Mass (Da)
	A7MJ65-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 87447AD9F09E55AA	FASTA	244	27,788
	10 20 30 40 50 60 MSVLDLHALN ALEKDARAGA LADTNAQLEA LSAEARVSWA LENLPGEFVL SSSFGIQAAV 70 80 90 100 110 120 CLHLVTQQRP DIPVILTDTG YLFPETYRFI DELTEKLNLN LKIYRAEQSP AWQEARYGKL 130 140 150 160 170 180 WEQGVEGIEK YNDINKVEPM NRALKELNAQ TWFAGLRRDQ SGSRANLPVL GVQRGVFKIL 190 200 210 220 230 240 PIIDWDNRTV YQYLQKHGLK YHPLWDEGYL SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton B., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000783 Genomic DNA. Translation: ABU75827.1.
RefSeq	YP_001436663.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5551840.
GenomeReviews	Gene locus ESA_00536 in contig CP000783_GR.
KEGG	esa:ESA_00536.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR004511. CysH. IPR002500. PAPS_reduct. IPR011800. PAPS_reductase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR00434. cysH. 1 hit. TIGR02057. PAPS_reductase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_ENTS8

Accession

Primary (citable) accession number: A7MJ65

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 2, 2007
Last modified:	November 25, 2008
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)