Reviewed, UniProtKB/Swiss-Prot A7MJ64 (CYSI_ENTS8)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] hemoprotein beta-component
      Short name=SiR-HP
      Short name=SiRHP
    EC=1.8.1.2

Gene names

Name:	cysI
Ordered Locus Names:	ESA_00535

Organism

Enterobacter sakazakii (strain ATCC BAA-894) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate By similarity. HAMAP MF_01540
Catalytic activity	H2S + 3 NADP+ + 3 H2O = sulfite + 3 NADPH. HAMAP MF_01540
Cofactor	Binds 1 siroheme per subunit By similarity. HAMAP MF_01540 Binds 1 4Fe-4S cluster per subunit By similarity. HAMAP MF_01540
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1. HAMAP MF_01540
Subunit structure	Alpha(8)-beta8. The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity. HAMAP MF_01540
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW hydrogen sulfide biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP
Cellular component	sulfite reductase complex (NADPH) Inferred from electronic annotation. Source: InterPro
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADP or NADPH binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	Sulfite reductase [NADPH] hemoprotein beta-component HAMAP MF_01540		PRO_1000068764
Sites
	Metal binding	434	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	440	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	479	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	483	1	Iron (siroheme axial ligand) By similarity
	Metal binding	483	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MJ64-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: BC6C15E898ADB63C	FASTA	570	63,950
	10 20 30 40 50 60 MSEKYPGPLV VEGKLSDAER MKRESNFLRG TIAEDLHDGL TGGFNGDNFL LIRFHGMYQQ 70 80 90 100 110 120 DDRDIRAERA AQKLEPRHAM MLRCRLPGGI MTPQQWLAID KFAEENTIYG SIRLTNRQTF 130 140 150 160 170 180 QYHGLLKKNV KPAHQMLHSV GLDALATAND MNRNVLCTSN PVESQLHAEA YEWAKKLSEH 190 200 210 220 230 240 LLPRTRAYAE IWLDQEKVAT TDEEPILGAT YLPRKFKTTV VVPPQNDVDL HANDMNFVAI 250 260 270 280 290 300 AENGKLVGFN LLVGGGLSIE HGNKKTYART ATEFGYIPLE HTLAVAEAVV TTQRDWGNRT 310 320 330 340 350 360 DRKNAKTKYT LERVGPDVFK AEVERRAGVT FEPVRPYEFT GRGDRIGWVK GIDDKWHLTL 370 380 390 400 410 420 FIENGRILDF PDKPLKTGLK EIARIHKGDF RLTANQNLIV AGVPEGDKAK IEQIARAHGL 430 440 450 460 470 480 MAGVTPQREN SMACVSFPTC PLAMAEAERF LPQFIDKVDG IMAKHGLAEE HIVLRVTGCP 490 500 510 520 530 540 NGCGRAMLAE LGLVGKAPGR YNLHLGGNRI GTRIPRMYRE NITESEILDN IDELVGRWAQ 550 560 570 EREPGEGFGD FTVRAGIIRP VLDPARDFWE

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton B., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000783 Genomic DNA. Translation: ABU75826.1.
RefSeq	YP_001436662.1.
3D structure databases
SMR	A7MJ64. Positions 81-570.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MJ64.
Genome annotation databases
GeneID	5551850.
GenomeReviews	Gene locus ESA_00535 in contig CP000783_GR.
KEGG	esa:ESA_00535.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0155.
HOGENOM	HBG626671.
OMA	PGDNSVD.
Family and domain databases
HAMAP	MF_01540. CysI. [Tree]
InterPro	IPR011786. CysI. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. [Graphical view]
Pfam	PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00397. SIROHAEM.
TIGRFAMs	TIGR02041. CysI. 1 hit.
PROSITE	PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSI_ENTS8

Accession

Primary (citable) accession number: A7MJ64

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 2, 2007
Last modified:	February 9, 2010
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)