Reviewed, UniProtKB/Swiss-Prot A7MJ63 (CYSJ_ENTS8)

Names and origin

Protein names

Recommended name:
    Sulfite reductase [NADPH] flavoprotein alpha-component
      Short name=SIR-FP
    EC=1.8.1.2

Gene names

Name:	cysJ
Ordered Locus Names:	ESA_00533

Organism

Enterobacter sakazakii (strain ATCC BAA-894) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavo-protein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component By similarity.
Catalytic activity	H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH.
Cofactor	Binds 1 FAD per subunit By similarity. Binds 1 FMN per subunit By similarity.
Subunit structure	Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein By similarity.
Sequence similarities	Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis Electron transport Transport
Ligand	FAD FMN Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	Sulfite reductase [NADPH] flavoprotein alpha-component		PRO_1000087636
Regions
	Domain	63 – 201	139	Flavodoxin-like
	Domain	235 – 449	215	FAD-binding FR-type
	Nucleotide binding	69 – 73	5	FMN By similarity
	Nucleotide binding	149 – 180	32	FMN By similarity
	Nucleotide binding	237 – 289	53	FAD By similarity
	Nucleotide binding	473 – 600	128	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MJ63-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 58E4C1E4C63B438B	FASTA	600	66,548
	10 20 30 40 50 60 MTTQAPPNAL LPLSPEQLAR LQTATHDFTP TQLAWLSGYF WGMVNQQPGA AVMQKPAAPA 70 80 90 100 110 120 SVITLISASQ TGNARRVAEA LRDDLLAAQL NVNLVNAGDY KFKQIAQEKL LIVVASTQGE 130 140 150 160 170 180 GDPPEEAVAL HKFLLSKKAP KLDGTAFAVF GLGDTSYEHF CQAGKDFDTR LAELGAERLL 190 200 210 220 230 240 DRVDADVEYQ AAAQAWRQRV VDVLKARVPK EAPSQAAITA SGAVNLVDST PYTKESPLTA 250 260 270 280 290 300 TLSVNQKITG RHSEKDVRHI EIDLGDAGLR YQPGDALGVW YQNDPALVQE LLELLWLKGD 310 320 330 340 350 360 EPVTVGEKTL PLSEALQWHF ELTVNTAAIV ENYATLTRSE ALLPLVGDKA KLQDYAARTP 370 380 390 400 410 420 IVDMVRFAPA QLEADQLLGL LRPLTPRLYS IASSQAEVEN EVHITVGVVR FDIEGRVRAG 430 440 450 460 470 480 GASSYLADRL EEDSEVRVFI EHNDNFRLPA TPETPVIMIG PGTGIAPFRA FMQQREADGA 490 500 510 520 530 540 TGKNWLFFGN PHFTEDFLYQ VEWQRYVKEG LLTRIDLAWS RDQDHKIYVQ DKIREQGAEL 550 560 570 580 590 600 WRWLQEGAHL YVCGDANRMA KDVEQALLEV IAAYGGMDAE AADEYLSELR VERRYQRDVY

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton B., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000783 Genomic DNA. Translation: ABU75824.1.
RefSeq	YP_001436661.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5551773.
GenomeReviews	Gene locus ESA_00533 in contig CP000783_GR.
KEGG	esa:ESA_00533.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_01541. [Tree]
InterPro	IPR010199. CysJ. IPR003097. FAD-binding_1. IPR001094. Flavdoxin_like. IPR008254. Flavodoxin/NO_synth. IPR001709. FPN_cyt_redctse. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00667. FAD_binding_1. 1 hit. PF00258. Flavodoxin_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00369. FLAVODOXIN. PR00371. FPNCR.
TIGRFAMs	TIGR01931. cysJ. 1 hit.
PROSITE	PS51384. FAD_FR. 1 hit. PS50902. FLAVODOXIN_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSJ_ENTS8

Accession

Primary (citable) accession number: A7MJ63

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	October 2, 2007
Last modified:	November 25, 2008
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)