A7MIS8 (RLMG_CROS8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase G
EC=2.1.1.174

Alternative name(s):
23S rRNA m2G1835 methyltransferase
rRNA (guanine-N(2)-)-methyltransferase RlmG

Gene names

Name:	rlmG
Ordered Locus Names:	ESA_03495

Organism

Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity. HAMAP MF_01859
Catalytic activity	S-adenosyl-L-methionine + guanosine(1835) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanosine(1835) in 23S rRNA. HAMAP MF_01859
Subcellular location	Cytoplasm Potential HAMAP MF_01859.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366451

Sequences

	Sequence		Length	Mass (Da)
	A7MIS8 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 3EFB618EAFF2D5CC	FASTA	376	42,016
	10 20 30 40 50 60 MSQVELLNQT LTLQRFPPMP EETPLQAWEA ADEYLLQQVE QPSGPVLIFN DSFGALACAL 70 80 90 100 110 120 AEVRPVSVND SFIAHQATRH NLRLNDIDDS LVTMQDSLSP LPDAPELVLM KIPKQLALLE 130 140 150 160 170 180 QQLRALRKVV TPQTRIIAGA KARDIHTSTL TLFEKILGPT TTTLAWKKAR LIHCAFSAPA 190 200 210 220 230 240 LADAPETLSW KLDGTPWTIH NHASVFSRTS LDIGARFFMQ HLPEAVEGEM VDLGCGNGVI 250 260 270 280 290 300 GLTLLAQNPQ AKVRFVDESY MAVASSRLNV ETNLPEAMER CEFQVNNALT GVEPESFHAV 310 320 330 340 350 360 LCNPPFHQQH AITDHIAWQM FQDARRCLKW GGELRIVGNR HLDYFRKLKK IFGNCTTVAT 370 NNKFVVLKAV KLRKSR

References

[1]

"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species." Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J. PLoS ONE 5:E9556-E9556(2010) [PubMed: 20221447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-894.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000783 Genomic DNA. Translation: ABU78710.1.
RefSeq	YP_001439546.1. NC_009778.1.
3D structure databases
ProteinModelPortal	A7MIS8.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MIS8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5551422.
GenomeReviews	Gene locus ESA_03495 in contig CP000783_GR.
KEGG	esa:ESA_03495.
PATRIC	20398694. VBICroSak107175_3108.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2813.
HOGENOM	HBG321931.
OMA	NRHLGYH.
ProtClustDB	CLSK2505749.
Family and domain databases
HAMAP	MF_01859. 23SrRNA_methyltr_G. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
KO	K11391.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_CROS8

Accession

Primary (citable) accession number: A7MIS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program