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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi489 – 4891MagnesiumUniRule annotation
Metal bindingi495 – 4951MagnesiumUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-3546-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:ESA_03557
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
ProteomesiUP000000260 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 711711Polyribonucleotide nucleotidyltransferasePRO_0000329634Add
BLAST

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation.UniRule annotation

Protein-protein interaction databases

STRINGi290339.ESA_03557.

Structurei

3D structure databases

ProteinModelPortaliA7MIN6.
SMRiA7MIN6. Positions 620-695.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini556 – 61560KHUniRule annotationAdd
BLAST
Domaini625 – 69369S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MIN6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNPIVRKFQ YGQHTVTLET GMMARQATAA VMVSMDDTAV FVTVVGAKKA
60 70 80 90 100
KPGQDFFPLT INYQERTYAA GRIPGGFFRR EGRPSEGETL IARLIDRPLR
110 120 130 140 150
PLFPEGFVNE VQVIATVVSV NPQVNPDIVA MIGASAALSL SGIPFNGPIG
160 170 180 190 200
AARVGYINDQ YVLNPTQDEL KSSKLDLVVA GTESAVLMVE SEAELLSEDQ
210 220 230 240 250
MLGAVVFGHE QQQVVIQNIN DLVKEAGKPR WDWQPEAANE ALNARVKALA
260 270 280 290 300
ESRLSDAYRI TDKQERYSQI DAIKADVIAA LQAEQAEGDA LDENELGDIL
310 320 330 340 350
HAIEKNVVRS RVLAGEPRID GREKDMIRGL DVRTGVLPRT HGSALFTRGE
360 370 380 390 400
TQALVTATLG TERDAQNIDE LMGERTDRFL FHYNFPPYSV GETGMVGSPK
410 420 430 440 450
RREIGHGRLA KRGVLAVMPA ADRFPYTVRV VSEITESNGS SSMASVCGAS
460 470 480 490 500
LALMDAGVPI KAAVAGIAMG LVKEGENFVV LSDILGDEDH LGDMDFKVAG
510 520 530 540 550
SREGITALQM DIKIEGITKE IMQVALNQAK GARLHILGVM EQAINAPRGD
560 570 580 590 600
ISQFAPRIHT IKISPDKIKD VIGKGGSVIR ALTEETGTTI EIEDDGTVKI
610 620 630 640 650
AATDGEKAKH AIRRIEEITA EIEVGRIYNG KVTRIVDFGA FVAIGGGKEG
660 670 680 690 700
LVHISQIADK RVEKVTDYLQ MGQEVPVKVL EVDRQGRVRL SIKEATEQTQ
710
EAAAPAAPEA E
Length:711
Mass (Da):76,970
Last modified:April 29, 2008 - v2
Checksum:iC3A68198B07D3A1D
GO

Sequence cautioni

The sequence ABU78771.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU78771.1. Different initiation.
RefSeqiYP_001439607.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU78771; ABU78771; ESA_03557.
KEGGiesa:ESA_03557.
PATRICi20398808. VBICroSak107175_3165.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU78771.1. Different initiation.
RefSeqiYP_001439607.1. NC_009778.1.

3D structure databases

ProteinModelPortaliA7MIN6.
SMRiA7MIN6. Positions 620-695.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290339.ESA_03557.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABU78771; ABU78771; ESA_03557.
KEGGiesa:ESA_03557.
PATRICi20398808. VBICroSak107175_3165.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-3546-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiPNP_CROS8
AccessioniPrimary (citable) accession number: A7MIN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: April 29, 2008
Last modified: April 1, 2015
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.