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Protein

Ribonuclease H

Gene

rnhA

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi10 – 101Magnesium 1UniRule annotation
Metal bindingi10 – 101Magnesium 2UniRule annotation
Metal bindingi48 – 481Magnesium 1UniRule annotation
Metal bindingi70 – 701Magnesium 1UniRule annotation
Metal bindingi134 – 1341Magnesium 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-3115-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease HUniRule annotation (EC:3.1.26.4UniRule annotation)
Short name:
RNase HUniRule annotation
Gene namesi
Name:rnhAUniRule annotation
Ordered Locus Names:ESA_03125
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
Proteomesi
  • UP000000260 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 158158Ribonuclease HPRO_1000074645Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi290339.ESA_03125.

Structurei

3D structure databases

ProteinModelPortaliA7MI34.
SMRiA7MI34. Positions 1-154.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 142142RNase HUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RNase H family.UniRule annotation
Contains 1 RNase H domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UMW. Bacteria.
COG0328. LUCA.
HOGENOMiHOG000040465.
KOiK03469.
OMAiLVTDSQY.
OrthoDBiEOG696BTR.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H.
InterProiIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MI34-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKQVEIFTD GSCLGNPGPG GYGAILRYKQ HERTFSAGYR LTTNNRMELM
60 70 80 90 100
AAIVSLEALR EHCIVTLSTD SQYVRQGITQ WIHNWKKRGW KTAEKKPVKN
110 120 130 140 150
VDLWQRLDAA LSQHEIKWEW VKGHAGHPEN ERCDELARAA AMAPTLEDTG

YQPEATAS
Length:158
Mass (Da):17,951
Last modified:October 2, 2007 - v1
Checksum:i413A0CCF9DDE8344
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU78352.1.
RefSeqiWP_004387414.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU78352; ABU78352; ESA_03125.
GeneIDi5549432.
KEGGiesa:ESA_03125.
PATRICi20397985. VBICroSak107175_2762.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU78352.1.
RefSeqiWP_004387414.1. NC_009778.1.

3D structure databases

ProteinModelPortaliA7MI34.
SMRiA7MI34. Positions 1-154.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290339.ESA_03125.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABU78352; ABU78352; ESA_03125.
GeneIDi5549432.
KEGGiesa:ESA_03125.
PATRICi20397985. VBICroSak107175_2762.

Phylogenomic databases

eggNOGiENOG4108UMW. Bacteria.
COG0328. LUCA.
HOGENOMiHOG000040465.
KOiK03469.
OMAiLVTDSQY.
OrthoDBiEOG696BTR.

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-3115-MONOMER.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00042. RNase_H.
InterProiIPR022892. RNaseH.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF00075. RNase_H. 1 hit.
[Graphical view]
SUPFAMiSSF53098. SSF53098. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiRNH_CROS8
AccessioniPrimary (citable) accession number: A7MI34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: October 2, 2007
Last modified: November 11, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.