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Protein

Fatty acid oxidation complex subunit alpha

Gene

fadJ

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of a hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities.UniRule annotation

Catalytic activityi

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O.UniRule annotation
(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.UniRule annotation
(S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.UniRule annotation

Pathwayi: fatty acid beta-oxidation

This protein is involved in the pathway fatty acid beta-oxidation, which is part of Lipid metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway fatty acid beta-oxidation and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei118Important for catalytic activityUniRule annotation1
Sitei140Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Lyase, Multifunctional enzyme, Oxidoreductase
Biological processFatty acid metabolism, Lipid degradation, Lipid metabolism
LigandNAD

Enzyme and pathway databases

BioCyciCSAK290339:G1G9O-831-MONOMER
UniPathwayiUPA00659

Names & Taxonomyi

Protein namesi
Recommended name:
Fatty acid oxidation complex subunit alphaUniRule annotation
Including the following 2 domains:
Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimeraseUniRule annotation (EC:4.2.1.17UniRule annotation, EC:5.1.2.3UniRule annotation)
3-hydroxyacyl-CoA dehydrogenaseUniRule annotation (EC:1.1.1.35UniRule annotation)
Gene namesi
Name:fadJUniRule annotation
Ordered Locus Names:ESA_00882
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeCronobacter
Proteomesi
  • UP000000260 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000694871 – 717Fatty acid oxidation complex subunit alphaAdd BLAST717

Proteomic databases

PRIDEiA7MH81

Interactioni

Subunit structurei

Heterotetramer of two alpha chains (FadJ) and two beta chains (FadI).UniRule annotation

Protein-protein interaction databases

STRINGi290339.ESA_00882

Structurei

3D structure databases

ProteinModelPortaliA7MH81
SMRiA7MH81
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 190Enoyl-CoA hydrataseUniRule annotationAdd BLAST190
Regioni306 – 7173-hydroxyacyl-CoA dehydrogenaseUniRule annotationAdd BLAST412

Sequence similaritiesi

In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.UniRule annotation
In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DYT Bacteria
COG1024 LUCA
COG1250 LUCA
HOGENOMiHOG000261346
KOiK01782
OMAiPFRYMDT

Family and domain databases

HAMAPiMF_01617 FadJ, 1 hit
InterProiView protein in InterPro
IPR006180 3-OHacyl-CoA_DH_CS
IPR006176 3-OHacyl-CoA_DH_NAD-bd
IPR006108 3HC_DH_C
IPR008927 6-PGluconate_DH-like_C_sf
IPR029045 ClpP/crotonase-like_dom_sf
IPR001753 Enoyl-CoA_hydra/iso
IPR012802 FadJ
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00725 3HCDH, 2 hits
PF02737 3HCDH_N, 1 hit
PF00378 ECH_1, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
SSF52096 SSF52096, 1 hit
TIGRFAMsiTIGR02440 FadJ, 1 hit
PROSITEiView protein in PROSITE
PS00067 3HCDH, 1 hit

Sequencei

Sequence statusi: Complete.

A7MH81-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESTSAFNLQ MRPDNVAVVT IDVPGEKMNT LKAEFARDVR AIVKTLRENR
60 70 80 90 100
DLAGVVFISA KPDNFIAGAD INMIAHCQSA QEAEALASQG QQIMAEIRAL
110 120 130 140 150
PVHVVAAIHG ACLGGGLELA LACHSRICTD DVKTLLGLPE VQLGLLPGSG
160 170 180 190 200
GTQRLPRLVG VSTALEMILA GKQLRPRQAL KAGLVDDVVP QSILLEAAAE
210 220 230 240 250
LAKKRRPAPR RLPVRERLLA GPIGRALLFR MVTQKTHQKT HGNYPAAQRI
260 270 280 290 300
IDVVRTGLEQ GSASGYQAEA RAFGELAMTP ESAALRGLFF ATTELKKETG
310 320 330 340 350
SEAAPRALHS VGVLGGGLMG GGIAYVTATK ARLPVRIKDI SEKGINHALK
360 370 380 390 400
YSWDLLEKKV RRRHLRASER DAQMALISAS TDYRGFHQRD IVVEAVFEDL
410 420 430 440 450
TLKQNMVAEI EAHTAPHTIF ASNTSSLPIG DIAAGATRPE QVIGLHYFSP
460 470 480 490 500
VDKMPLVEVI PHAGTSAETI ATTVQLAKKQ GKTPIVVADC AGFYVNRILA
510 520 530 540 550
PYINEAMRCL MEGESIEKID EALVKRGFPV GPIQLLDEVG IDVGTKIMPV
560 570 580 590 600
LERAYGPRFS APGDAVAAIL NDDRKGRKNG RGFYLYPAKG RKSKKQVDPA
610 620 630 640 650
VYGLIGVKPG GKLSGEEIAE RCVMMMLNEA ARCLDEGVVR SARDGDIGAV
660 670 680 690 700
FGIGFPPFLG GPFRYMDTLG AAAMATTLTR LATRYGDRFT PCDRLLRMAQ
710
TGQTFWLAGN LQAEMTV
Length:717
Mass (Da):77,255
Last modified:October 2, 2007 - v1
Checksum:iFAE2FA3A79ADBF70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA Translation: ABU76152.1
RefSeqiWP_012124133.1, NC_009778.1

Genome annotation databases

EnsemblBacteriaiABU76152; ABU76152; ESA_00882
KEGGiesa:ESA_00882
PATRICifig|290339.8.peg.782

Similar proteinsi

Entry informationi

Entry nameiFADJ_CROS8
AccessioniPrimary (citable) accession number: A7MH81
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 2, 2007
Last modified: May 23, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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