Reviewed, UniProtKB/Swiss-Prot A7MH81 (FADJ_ENTS8)

Names and origin

Protein names

Recommended name:
    Fatty acid oxidation complex subunit alpha
Including the following 2 domains:
    1- Recommended name:
            Enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase
              EC=4.2.1.17
              EC=5.1.2.3
    2- Recommended name:
            3-hydroxyacyl-CoA dehydrogenase
              EC=1.1.1.35

Gene names

Name:	fadJ
Ordered Locus Names:	ESA_00882

Organism

Enterobacter sakazakii (strain ATCC BAA-894) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	717 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities By similarity.
Catalytic activity	(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O. HAMAP MF_01617 (S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH. HAMAP MF_01617 (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA. HAMAP MF_01617
Pathway	Lipid metabolism; fatty acid beta-oxidation. HAMAP MF_01617
Subunit structure	Heterotetramer of two alpha chains (fadJ) and two beta chains (fadI) By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family. In the central section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid degradation Lipid metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Isomerase Lyase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid beta-oxidation Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	fatty acid beta-oxidation multienzyme complex Inferred from electronic annotation. Source: InterPro
Molecular function	3-hydroxyacyl-CoA dehydrogenase activity Inferred from electronic annotation. Source: HAMAP 3-hydroxybutyryl-CoA epimerase activity Inferred from electronic annotation. Source: HAMAP NAD or NADH binding Inferred from electronic annotation. Source: InterPro enoyl-CoA hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 717	717	Fatty acid oxidation complex subunit alpha HAMAP MF_01617		PRO_1000069487
Regions
	Region	1 – 190	190	Enoyl-CoA hydratase By similarity
	Region	306 – 717	412	3-hydroxyacyl-CoA dehydrogenase By similarity
Sites
	Site	118	1	Important for catalytic activity By similarity
	Site	140	1	Important for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MH81-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: FAE2FA3A79ADBF70	FASTA	717	77,255
	10 20 30 40 50 60 MESTSAFNLQ MRPDNVAVVT IDVPGEKMNT LKAEFARDVR AIVKTLRENR DLAGVVFISA 70 80 90 100 110 120 KPDNFIAGAD INMIAHCQSA QEAEALASQG QQIMAEIRAL PVHVVAAIHG ACLGGGLELA 130 140 150 160 170 180 LACHSRICTD DVKTLLGLPE VQLGLLPGSG GTQRLPRLVG VSTALEMILA GKQLRPRQAL 190 200 210 220 230 240 KAGLVDDVVP QSILLEAAAE LAKKRRPAPR RLPVRERLLA GPIGRALLFR MVTQKTHQKT 250 260 270 280 290 300 HGNYPAAQRI IDVVRTGLEQ GSASGYQAEA RAFGELAMTP ESAALRGLFF ATTELKKETG 310 320 330 340 350 360 SEAAPRALHS VGVLGGGLMG GGIAYVTATK ARLPVRIKDI SEKGINHALK YSWDLLEKKV 370 380 390 400 410 420 RRRHLRASER DAQMALISAS TDYRGFHQRD IVVEAVFEDL TLKQNMVAEI EAHTAPHTIF 430 440 450 460 470 480 ASNTSSLPIG DIAAGATRPE QVIGLHYFSP VDKMPLVEVI PHAGTSAETI ATTVQLAKKQ 490 500 510 520 530 540 GKTPIVVADC AGFYVNRILA PYINEAMRCL MEGESIEKID EALVKRGFPV GPIQLLDEVG 550 560 570 580 590 600 IDVGTKIMPV LERAYGPRFS APGDAVAAIL NDDRKGRKNG RGFYLYPAKG RKSKKQVDPA 610 620 630 640 650 660 VYGLIGVKPG GKLSGEEIAE RCVMMMLNEA ARCLDEGVVR SARDGDIGAV FGIGFPPFLG 670 680 690 700 710 GPFRYMDTLG AAAMATTLTR LATRYGDRFT PCDRLLRMAQ TGQTFWLAGN LQAEMTV

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton B., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000783 Genomic DNA. Translation: ABU76152.1.
RefSeq	YP_001436988.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7MH81.
Genome annotation databases
GeneID	5550271.
GenomeReviews	Gene locus ESA_00882 in contig CP000783_GR.
KEGG	esa:ESA_00882.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	GDRFTPC.
Family and domain databases
HAMAP	MF_01617. [Tree]
InterPro	IPR006180. 3-OHacyl-CoA_DH_CS. IPR006176. 3-OHacyl-CoA_DH_NAD-bd. IPR006108. 3HC_DH_C. IPR001753. Crotonase_core. IPR013328. DH_multihelical. IPR018376. Enoyl-CoA_hyd/isom_CS. IPR012802. FadJ. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 2 hits.
Pfam	PF00725. 3HCDH. 2 hits. PF02737. 3HCDH_N. 1 hit. PF00378. ECH. 1 hit. [Graphical view]
TIGRFAMs	TIGR02440. FadJ. 1 hit.
PROSITE	PS00067. 3HCDH. 1 hit. PS00166. ENOYL_COA_HYDRATASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FADJ_ENTS8

Accession

Primary (citable) accession number: A7MH81

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	October 2, 2007
Last modified:	November 3, 2009
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)