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A7MGZ5

- MLTF_CROS8

UniProt

A7MGZ5 - MLTF_CROS8

Protein

Membrane-bound lytic murein transglycosylase F

Gene

mltF

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.UniRule annotation

    Catalytic activityi

    Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei314 – 3141UniRule annotation

    GO - Molecular functioni

    1. carbon-oxygen lyase activity, acting on polysaccharides Source: UniProtKB-EC
    2. lytic transglycosylase activity Source: UniProtKB-HAMAP
    3. transporter activity Source: InterPro

    GO - Biological processi

    1. cell wall macromolecule catabolic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciCSAK290339:GJ80-697-MONOMER.

    Protein family/group databases

    CAZyiGH23. Glycoside Hydrolase Family 23.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Membrane-bound lytic murein transglycosylase FUniRule annotation (EC:4.2.2.n1UniRule annotation)
    Alternative name(s):
    Murein lyase FUniRule annotation
    Gene namesi
    Name:mltFUniRule annotation
    Ordered Locus Names:ESA_00703
    OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
    Taxonomic identifieri290339 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
    ProteomesiUP000000260: Chromosome

    Subcellular locationi

    Cell outer membrane; Peripheral membrane protein
    Note: Attached to the inner leaflet of the outer membrane.UniRule annotation

    GO - Cellular componenti

    1. cell outer membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell outer membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232UniRule annotationAdd
    BLAST
    Chaini33 – 519487Membrane-bound lytic murein transglycosylase FPRO_0000353936Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi290339.ESA_00703.

    Structurei

    3D structure databases

    ProteinModelPortaliA7MGZ5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni33 – 269237Non-LT domainUniRule annotationAdd
    BLAST
    Regioni270 – 519250LT domainUniRule annotationAdd
    BLAST

    Domaini

    The N-terminal domain does not have lytic activity and probably modulates enzymatic activity. The C-terminal domain is the catalytic active domain.UniRule annotation

    Sequence similaritiesi

    In the N-terminal section; belongs to the bacterial solute-binding protein 3 family.UniRule annotation
    In the C-terminal section; belongs to the transglycosylase Slt family.UniRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4623.
    HOGENOMiHOG000218316.
    OMAiNIRRYYD.
    OrthoDBiEOG62C9FB.

    Family and domain databases

    HAMAPiMF_02016. MltF.
    InterProiIPR023346. Lysozyme-like_dom.
    IPR023703. MltF.
    IPR001638. SBP_bac_3.
    IPR008258. TGlycosylase-like_SLT.
    [Graphical view]
    PfamiPF00497. SBP_bac_3. 1 hit.
    PF01464. SLT. 1 hit.
    [Graphical view]
    SMARTiSM00062. PBPb. 1 hit.
    [Graphical view]
    SUPFAMiSSF53955. SSF53955. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A7MGZ5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKLKLNYLL IGVVTLLLAV ALWPAIPWSG KADNRIAAIQ ARGVLRVSTI    50
    ATPLTMYRAG DTMSGLDYEL SQAFADYLGV KLKITVRQNI SQLFDDLDNN 100
    DADLLAAGLV YNEARSQHYQ AGPIYYSVSQ QMVYRVGSLR PRSLASIKEG 150
    QLTIAPGHVA QSELEQLKAS KYPDLTWRVD PKLSSNELML QVAQGTLDYT 200
    IADSVAISLF QRVHPQLAVA LDLSDEQPVT WFSRRSSDNS LSAALLDFFN 250
    QINEDGTLAR LEEKYLGHGN DFDYVDTRSF LRAVDSVLPE LQPLFEKYAT 300
    DIDWRLLAAI SYQESHWDSQ ATSPTGVRGL MMLTRNTAQS LGLTDRLDAE 350
    QSISGGARYL KDMMSKVPES VPEEERIWFA LAAYNMGYAH MLDARALTAK 400
    QKGNPDSWSD VKQRLPLLSQ RPYYSKLTYG YARGHEAYAY VENIRKYQIS 450
    LVGYLLEKEK QAAAAAQPKL VQSYPTNITP QGLFPSPFSS LPLGPFSQAG 500
    AGGKTHSALP NTLSPVSPR 519
    Length:519
    Mass (Da):57,617
    Last modified:October 2, 2007 - v1
    Checksum:i818FB065D8B03B13
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000783 Genomic DNA. Translation: ABU75981.1.
    RefSeqiWP_012124025.1. NC_009778.1.
    YP_001436817.1. NC_009778.1.

    Genome annotation databases

    EnsemblBacteriaiABU75981; ABU75981; ESA_00703.
    GeneIDi5548883.
    KEGGiesa:ESA_00703.
    PATRICi20393627. VBICroSak107175_0621.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000783 Genomic DNA. Translation: ABU75981.1 .
    RefSeqi WP_012124025.1. NC_009778.1.
    YP_001436817.1. NC_009778.1.

    3D structure databases

    ProteinModelPortali A7MGZ5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 290339.ESA_00703.

    Protein family/group databases

    CAZyi GH23. Glycoside Hydrolase Family 23.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABU75981 ; ABU75981 ; ESA_00703 .
    GeneIDi 5548883.
    KEGGi esa:ESA_00703.
    PATRICi 20393627. VBICroSak107175_0621.

    Phylogenomic databases

    eggNOGi COG4623.
    HOGENOMi HOG000218316.
    OMAi NIRRYYD.
    OrthoDBi EOG62C9FB.

    Enzyme and pathway databases

    BioCyci CSAK290339:GJ80-697-MONOMER.

    Family and domain databases

    HAMAPi MF_02016. MltF.
    InterProi IPR023346. Lysozyme-like_dom.
    IPR023703. MltF.
    IPR001638. SBP_bac_3.
    IPR008258. TGlycosylase-like_SLT.
    [Graphical view ]
    Pfami PF00497. SBP_bac_3. 1 hit.
    PF01464. SLT. 1 hit.
    [Graphical view ]
    SMARTi SM00062. PBPb. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53955. SSF53955. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
      Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
      PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-894.

    Entry informationi

    Entry nameiMLTF_CROS8
    AccessioniPrimary (citable) accession number: A7MGZ5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 25, 2008
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3