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A7MGZ5

- MLTF_CROS8

UniProt

A7MGZ5 - MLTF_CROS8

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Protein

Membrane-bound lytic murein transglycosylase F

Gene

mltF

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.UniRule annotation

Catalytic activityi

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei314 – 3141UniRule annotation

GO - Molecular functioni

  1. carbon-oxygen lyase activity, acting on polysaccharides Source: UniProtKB-EC
  2. lytic transglycosylase activity Source: UniProtKB-HAMAP
  3. transporter activity Source: InterPro

GO - Biological processi

  1. cell wall macromolecule catabolic process Source: UniProtKB-HAMAP
  2. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-697-MONOMER.

Protein family/group databases

CAZyiGH23. Glycoside Hydrolase Family 23.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound lytic murein transglycosylase FUniRule annotation (EC:4.2.2.n1UniRule annotation)
Alternative name(s):
Murein lyase FUniRule annotation
Gene namesi
Name:mltFUniRule annotation
Ordered Locus Names:ESA_00703
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
ProteomesiUP000000260: Chromosome

Subcellular locationi

Cell outer membrane; Peripheral membrane protein
Note: Attached to the inner leaflet of the outer membrane.UniRule annotation

GO - Cellular componenti

  1. cell outer membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232UniRule annotationAdd
BLAST
Chaini33 – 519487Membrane-bound lytic murein transglycosylase FPRO_0000353936Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290339.ESA_00703.

Structurei

3D structure databases

ProteinModelPortaliA7MGZ5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 269237Non-LT domainUniRule annotationAdd
BLAST
Regioni270 – 519250LT domainUniRule annotationAdd
BLAST

Domaini

The N-terminal domain does not have lytic activity and probably modulates enzymatic activity. The C-terminal domain is the catalytic active domain.UniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the bacterial solute-binding protein 3 family.UniRule annotation
In the C-terminal section; belongs to the transglycosylase Slt family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4623.
HOGENOMiHOG000218316.
OMAiNIRRYYD.
OrthoDBiEOG62C9FB.

Family and domain databases

HAMAPiMF_02016. MltF.
InterProiIPR023346. Lysozyme-like_dom.
IPR023703. MltF.
IPR001638. SBP_bac_3.
IPR008258. TGlycosylase-like_SLT.
[Graphical view]
PfamiPF00497. SBP_bac_3. 1 hit.
PF01464. SLT. 1 hit.
[Graphical view]
SMARTiSM00062. PBPb. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A7MGZ5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKLKLNYLL IGVVTLLLAV ALWPAIPWSG KADNRIAAIQ ARGVLRVSTI
60 70 80 90 100
ATPLTMYRAG DTMSGLDYEL SQAFADYLGV KLKITVRQNI SQLFDDLDNN
110 120 130 140 150
DADLLAAGLV YNEARSQHYQ AGPIYYSVSQ QMVYRVGSLR PRSLASIKEG
160 170 180 190 200
QLTIAPGHVA QSELEQLKAS KYPDLTWRVD PKLSSNELML QVAQGTLDYT
210 220 230 240 250
IADSVAISLF QRVHPQLAVA LDLSDEQPVT WFSRRSSDNS LSAALLDFFN
260 270 280 290 300
QINEDGTLAR LEEKYLGHGN DFDYVDTRSF LRAVDSVLPE LQPLFEKYAT
310 320 330 340 350
DIDWRLLAAI SYQESHWDSQ ATSPTGVRGL MMLTRNTAQS LGLTDRLDAE
360 370 380 390 400
QSISGGARYL KDMMSKVPES VPEEERIWFA LAAYNMGYAH MLDARALTAK
410 420 430 440 450
QKGNPDSWSD VKQRLPLLSQ RPYYSKLTYG YARGHEAYAY VENIRKYQIS
460 470 480 490 500
LVGYLLEKEK QAAAAAQPKL VQSYPTNITP QGLFPSPFSS LPLGPFSQAG
510
AGGKTHSALP NTLSPVSPR
Length:519
Mass (Da):57,617
Last modified:October 2, 2007 - v1
Checksum:i818FB065D8B03B13
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000783 Genomic DNA. Translation: ABU75981.1.
RefSeqiYP_001436817.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU75981; ABU75981; ESA_00703.
GeneIDi5548883.
KEGGiesa:ESA_00703.
PATRICi20393627. VBICroSak107175_0621.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000783 Genomic DNA. Translation: ABU75981.1 .
RefSeqi YP_001436817.1. NC_009778.1.

3D structure databases

ProteinModelPortali A7MGZ5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290339.ESA_00703.

Protein family/group databases

CAZyi GH23. Glycoside Hydrolase Family 23.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABU75981 ; ABU75981 ; ESA_00703 .
GeneIDi 5548883.
KEGGi esa:ESA_00703.
PATRICi 20393627. VBICroSak107175_0621.

Phylogenomic databases

eggNOGi COG4623.
HOGENOMi HOG000218316.
OMAi NIRRYYD.
OrthoDBi EOG62C9FB.

Enzyme and pathway databases

BioCyci CSAK290339:GJ80-697-MONOMER.

Family and domain databases

HAMAPi MF_02016. MltF.
InterProi IPR023346. Lysozyme-like_dom.
IPR023703. MltF.
IPR001638. SBP_bac_3.
IPR008258. TGlycosylase-like_SLT.
[Graphical view ]
Pfami PF00497. SBP_bac_3. 1 hit.
PF01464. SLT. 1 hit.
[Graphical view ]
SMARTi SM00062. PBPb. 1 hit.
[Graphical view ]
SUPFAMi SSF53955. SSF53955. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiMLTF_CROS8
AccessioniPrimary (citable) accession number: A7MGZ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: October 2, 2007
Last modified: October 29, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3