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A7MGS0

- GLND_CROS8

UniProt

A7MGS0 - GLND_CROS8

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-3166-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:ESA_03176
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
ProteomesiUP000000260: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 892892Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000022342Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290339.ESA_03176.

Structurei

3D structure databases

ProteinModelPortaliA7MGS0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini470 – 603134HDUniRule annotationAdd
BLAST
Domaini711 – 79080ACT 1UniRule annotationAdd
BLAST
Domaini818 – 89275ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 351351UridylyltransferaseAdd
BLAST
Regioni352 – 710359Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MGS0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMSNTLPEQS VNTTLAPLPG QPDYPATWPA DALNCAQIKQ HLDAFQQWLG
60 70 80 90 100
AAFDDGYTAE QLIEARTEFI DQLLQRLWIA AGFGDTPDTA LVAVGGYGRG
110 120 130 140 150
ELHPLSDIDL LILSRKRLSE AQAQKVGELL TLLWDVKLEV GHSVRTLEEC
160 170 180 190 200
LLEGLSDLTV ATNLIESRLL IGDVALFLEL QKHIFSDGFW PSEKFFAAKV
210 220 230 240 250
EEQNERHQRY HGTSYNLEPD IKSSPGGLRD IHTLQWVARR HFGATSLREM
260 270 280 290 300
VGFGFLTEAE RNELDECQHL LWRIRFALHL ELNRYDNRLL FDRQLSVAQR
310 320 330 340 350
LRYEGEGNEP VEHMMKDFYR VTRRVGELNQ MLLQLFDEAI LALTTDEKPR
360 370 380 390 400
VLDDDFQLRG TLIDLRDETL FIREPQAILR MFYMMVRNRD ITGIYSTTLR
410 420 430 440 450
HLRHARRHLK QPLCYIPEAR SLFLAMLRHP GAVSRGLLPM HRHSVLWAYM
460 470 480 490 500
PQWSHIVGQM QFDLFHAYTV DEHTIRVLLK LESFAKEETR AKHPLCVDLW
510 520 530 540 550
PRLSHPELIL IAALFHDIAK GRGGDHSVLG AQDILKFAEL HGLNSRETQL
560 570 580 590 600
VAWLVRHHLL MSVTAQRRDI QDPEVIKQFA EEVQTENRLR FLVCLTVADI
610 620 630 640 650
CATNETLWNS WKQSLLRELY FATEKQLRRG MQSTPDMRER VRHHQLQALA
660 670 680 690 700
LLRMENIDEE ALHHIWGRCR ANYFVRHSPN QLAWHARHLL RHDLSKPLIL
710 720 730 740 750
LSPQATRGGT EIFIWSPDRP YLFAAVCAEL DRRNLSVHDA QIFTTRDDMA
760 770 780 790 800
MDTFIVLEPD GSPLSPDRHE AIRHGLEQAI TQRTWQPPQP RRQPAKLRHF
810 820 830 840 850
TVETEVNFLP THTDRKSFLE LIALDQPGLL ARVGQVFADL GISLHGARIS
860 870 880 890
TIGERVEDLF IIATADRRGL NNLLQQEVRQ RLTEDLNPND KV
Length:892
Mass (Da):103,003
Last modified:October 2, 2007 - v1
Checksum:i99E2B1D75E7F6B4E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000783 Genomic DNA. Translation: ABU78398.1.
RefSeqiYP_001439234.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU78398; ABU78398; ESA_03176.
GeneIDi5549477.
KEGGiesa:ESA_03176.
PATRICi20398085. VBICroSak107175_2807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000783 Genomic DNA. Translation: ABU78398.1 .
RefSeqi YP_001439234.1. NC_009778.1.

3D structure databases

ProteinModelPortali A7MGS0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290339.ESA_03176.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABU78398 ; ABU78398 ; ESA_03176 .
GeneIDi 5549477.
KEGGi esa:ESA_03176.
PATRICi 20398085. VBICroSak107175_2807.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci CSAK290339:GJ80-3166-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiGLND_CROS8
AccessioniPrimary (citable) accession number: A7MGS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: October 29, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3