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A7MGS0

- GLND_CROS8

UniProt

A7MGS0 - GLND_CROS8

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, ESA_03176
Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-3166-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:ESA_03176
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
ProteomesiUP000000260: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 892892Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_1000022342Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290339.ESA_03176.

Structurei

3D structure databases

ProteinModelPortaliA7MGS0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini470 – 603134HDAdd
BLAST
Domaini711 – 79080ACT 1Add
BLAST
Domaini818 – 89275ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 351351UridylyltransferaseUniRule annotationAdd
BLAST
Regioni352 – 710359Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MGS0-1 [UniParc]FASTAAdd to Basket

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MMSNTLPEQS VNTTLAPLPG QPDYPATWPA DALNCAQIKQ HLDAFQQWLG    50
AAFDDGYTAE QLIEARTEFI DQLLQRLWIA AGFGDTPDTA LVAVGGYGRG 100
ELHPLSDIDL LILSRKRLSE AQAQKVGELL TLLWDVKLEV GHSVRTLEEC 150
LLEGLSDLTV ATNLIESRLL IGDVALFLEL QKHIFSDGFW PSEKFFAAKV 200
EEQNERHQRY HGTSYNLEPD IKSSPGGLRD IHTLQWVARR HFGATSLREM 250
VGFGFLTEAE RNELDECQHL LWRIRFALHL ELNRYDNRLL FDRQLSVAQR 300
LRYEGEGNEP VEHMMKDFYR VTRRVGELNQ MLLQLFDEAI LALTTDEKPR 350
VLDDDFQLRG TLIDLRDETL FIREPQAILR MFYMMVRNRD ITGIYSTTLR 400
HLRHARRHLK QPLCYIPEAR SLFLAMLRHP GAVSRGLLPM HRHSVLWAYM 450
PQWSHIVGQM QFDLFHAYTV DEHTIRVLLK LESFAKEETR AKHPLCVDLW 500
PRLSHPELIL IAALFHDIAK GRGGDHSVLG AQDILKFAEL HGLNSRETQL 550
VAWLVRHHLL MSVTAQRRDI QDPEVIKQFA EEVQTENRLR FLVCLTVADI 600
CATNETLWNS WKQSLLRELY FATEKQLRRG MQSTPDMRER VRHHQLQALA 650
LLRMENIDEE ALHHIWGRCR ANYFVRHSPN QLAWHARHLL RHDLSKPLIL 700
LSPQATRGGT EIFIWSPDRP YLFAAVCAEL DRRNLSVHDA QIFTTRDDMA 750
MDTFIVLEPD GSPLSPDRHE AIRHGLEQAI TQRTWQPPQP RRQPAKLRHF 800
TVETEVNFLP THTDRKSFLE LIALDQPGLL ARVGQVFADL GISLHGARIS 850
TIGERVEDLF IIATADRRGL NNLLQQEVRQ RLTEDLNPND KV 892
Length:892
Mass (Da):103,003
Last modified:October 2, 2007 - v1
Checksum:i99E2B1D75E7F6B4E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000783 Genomic DNA. Translation: ABU78398.1.
RefSeqiWP_012125716.1. NC_009778.1.
YP_001439234.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU78398; ABU78398; ESA_03176.
GeneIDi5549477.
KEGGiesa:ESA_03176.
PATRICi20398085. VBICroSak107175_2807.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000783 Genomic DNA. Translation: ABU78398.1 .
RefSeqi WP_012125716.1. NC_009778.1.
YP_001439234.1. NC_009778.1.

3D structure databases

ProteinModelPortali A7MGS0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290339.ESA_03176.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABU78398 ; ABU78398 ; ESA_03176 .
GeneIDi 5549477.
KEGGi esa:ESA_03176.
PATRICi 20398085. VBICroSak107175_2807.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci CSAK290339:GJ80-3166-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiGLND_CROS8
AccessioniPrimary (citable) accession number: A7MGS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: September 3, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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