Reviewed, UniProtKB/Swiss-Prot A7MFZ8 (GHRA_ENTS8)

Names and origin

Protein names

Recommended name:
    Glyoxylate/hydroxypyruvate reductase A
    EC=1.1.1.79
    EC=1.1.1.81
Alternative name(s):
    2-ketoacid reductase

Gene names

Name:	ghrA
Ordered Locus Names:	ESA_02341

Organism

Enterobacter sakazakii (strain ATCC BAA-894) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity.
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01666 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01666
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	cofactor binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Glyoxylate/hydroxypyruvate reductase A HAMAP MF_01666		PRO_0000348364
Sites
	Active site	228	1	By similarity
	Active site	276	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MFZ8-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: FD47ECE191C76476	FASTA	313	35,010
	10 20 30 40 50 60 MDILFYHPTF DISEWAALLQ RHLPHARLRA WQPGDNAAAD YALVWHPPYD MLRGRVDLKA 70 80 90 100 110 120 IFALGAGVDS LLSRLNEYPD LLPSGVPLYR MEDTGMAAQM QEYAISQVLH WFRRFDDYAA 130 140 150 160 170 180 LQREARWEPL EEYRHADFQV GVLGAGVLGG QVAQSLSRMG FPVRCWSRSC KALPGVESFA 190 200 210 220 230 240 GPDELSAFLH GTRVLINLLP NTPETVGIIN AQLLAALNDN AYVLNLARGV HLVEEDLLAA 250 260 270 280 290 300 LDRGKVKGAM LDVFQQEPLP HESPLWRHPR VRITPHVAAV TRPEEAVAFI AQSITRIERG 310 DIPPNQVDVA RGY

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton B., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000783 Genomic DNA. Translation: ABU77590.1.
RefSeq	YP_001438426.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5551295.
GenomeReviews	Gene locus ESA_02341 in contig CP000783_GR.
KEGG	esa:ESA_02341.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A7MFZ8. YALVWRA.
Family and domain databases
HAMAP	MF_01666. [Tree]
InterPro	IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRA_ENTS8

Accession

Primary (citable) accession number: A7MFZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	October 2, 2007
Last modified:	June 16, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)