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A7MFT1 (NAGZ_CROS8) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-hexosaminidase

EC=3.2.1.52
Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase
Gene names
Name:nagZ
Ordered Locus Names:ESA_02237
OrganismCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]
Taxonomic identifier290339 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter

Protein attributes

Sequence length341 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays a role in peptidoglycan recycling by cleaving the terminal beta-1,4-linked N-acetylglucosamine (GlcNAc) from peptide-linked peptidoglycan fragments, giving rise to free GlcNAc, anhydro-N-acetylmuramic acid and anhydro-N-acetylmuramic acid-linked peptides By similarity. HAMAP-Rule MF_00364

Catalytic activity

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP-Rule MF_00364

Pathway

Cell wall biogenesis; peptidoglycan recycling. HAMAP-Rule MF_00364

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00364.

Sequence similarities

Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 341341Beta-hexosaminidase HAMAP-Rule MF_00364
PRO_1000005653

Regions

Region163 – 1642Substrate binding By similarity

Sites

Active site1761Proton donor/acceptor By similarity
Active site2481Nucleophile By similarity
Binding site621Substrate By similarity
Binding site701Substrate By similarity
Binding site1331Substrate By similarity
Site1741Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
A7MFT1 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: FC244DFB713E6EE3

FASTA34137,576
        10         20         30         40         50         60 
MGPVMLDVEG FELDAEEREI LAHPLVGGLI LFTRNYHDPE QLRELVRQIR SSARNHLVIA 

        70         80         90        100        110        120 
VDQEGGRVQR FREGFTRLPA AQSFAALLGM DAGGKLAEEA GWLMACEMIA MDIDISFAPV 

       130        140        150        160        170        180 
LDVGHLSAAI GERSFHEEPE NVLAMAKRFI TGMQSAGMKA TGKHFPGHGA VSADSHKETP 

       190        200        210        220        230        240 
RDPRPAAEIR RHDMLPFREL ISYNMLDAVM PAHVVYSDVD PLPASGSPYW LKQVLRKELG 

       250        260        270        280        290        300 
FDGVIFSDDL SMEGAAILGS YAERGQAALD AGCDMILVCN NRKGAVSVLD NLSPIKAERV 

       310        320        330        340 
TRLYHKGVIT RQELMASARW KQVSGELTAL HERWQTHKAG Q 

« Hide

References

[1]"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-894.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000783 Genomic DNA. Translation: ABU77486.1.
RefSeqYP_001438322.1. NC_009778.1.

3D structure databases

ProteinModelPortalA7MFT1.
SMRA7MFT1. Positions 1-334.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290339.ESA_02237.

Protein family/group databases

CAZyGH3. Glycoside Hydrolase Family 3.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU77486; ABU77486; ESA_02237.
GeneID5551819.
KEGGesa:ESA_02237.
PATRIC20396421. VBICroSak107175_1998.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1472.
HOGENOMHOG000248526.
KOK01207.
OMAAGFSSYW.
OrthoDBEOG6BCT06.
ProtClustDBPRK05337.

Enzyme and pathway databases

BioCycCSAK290339:GJ80-2229-MONOMER.
UniPathwayUPA00544.

Family and domain databases

Gene3D3.20.20.300. 1 hit.
HAMAPMF_00364. NagZ.
InterProIPR022956. Beta_hexosaminidase_bac.
IPR019800. Glyco_hydro_3_AS.
IPR001764. Glyco_hydro_3_N.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00933. Glyco_hydro_3. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00775. GLYCOSYL_HYDROL_F3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNAGZ_CROS8
AccessionPrimary (citable) accession number: A7MFT1
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: April 16, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries