Reviewed, UniProtKB/Swiss-Prot A7MFT1 (NAGZ_ENTS8)

Names and origin

Protein names

Recommended name:
    Beta-hexosaminidase
    EC=3.2.1.52
Alternative name(s):
    N-acetyl-beta-glucosaminidase
    Beta-N-acetylhexosaminidase

Gene names

Name:	nagZ
Ordered Locus Names:	ESA_02237

Organism

Enterobacter sakazakii (strain ATCC BAA-894) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides By similarity. HAMAP MF_00364
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP MF_00364
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP MF_00364
Subcellular location	Cytoplasm By similarity HAMAP MF_00364.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan turnover Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Beta-hexosaminidase HAMAP MF_00364		PRO_1000005653
Sites
	Active site	248	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MFT1-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: FC244DFB713E6EE3	FASTA	341	37,576
	10 20 30 40 50 60 MGPVMLDVEG FELDAEEREI LAHPLVGGLI LFTRNYHDPE QLRELVRQIR SSARNHLVIA 70 80 90 100 110 120 VDQEGGRVQR FREGFTRLPA AQSFAALLGM DAGGKLAEEA GWLMACEMIA MDIDISFAPV 130 140 150 160 170 180 LDVGHLSAAI GERSFHEEPE NVLAMAKRFI TGMQSAGMKA TGKHFPGHGA VSADSHKETP 190 200 210 220 230 240 RDPRPAAEIR RHDMLPFREL ISYNMLDAVM PAHVVYSDVD PLPASGSPYW LKQVLRKELG 250 260 270 280 290 300 FDGVIFSDDL SMEGAAILGS YAERGQAALD AGCDMILVCN NRKGAVSVLD NLSPIKAERV 310 320 330 340 TRLYHKGVIT RQELMASARW KQVSGELTAL HERWQTHKAG Q

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton B., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000783 Genomic DNA. Translation: ABU77486.1.
RefSeq	YP_001438322.1.
3D structure databases
SMR	A7MFT1. Positions 1-334.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MFT1.
Genome annotation databases
GeneID	5551819.
GenomeReviews	Gene locus ESA_02237 in contig CP000783_GR.
KEGG	esa:ESA_02237.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1472.
HOGENOM	HBG617255.
OMA	ADSHIAQ.
Family and domain databases
HAMAP	MF_00364. NagZ. [Tree]
InterPro	IPR019800. Glyco_hydro_3_AS. IPR001764. Glyco_hydro_3_N. IPR017853. Glyco_hydro_catalytic_core. [Graphical view]
Gene3D	G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
Pfam	PF00933. Glyco_hydro_3. 1 hit. [Graphical view]
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGZ_ENTS8

Accession

Primary (citable) accession number: A7MFT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 2, 2007
Last modified:	January 19, 2010
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)