A7MFT1 (NAGZ_CROS8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-hexosaminidase
EC=3.2.1.52

Alternative name(s):
Beta-N-acetylhexosaminidase
N-acetyl-beta-glucosaminidase

Gene names

Name:	nagZ
Ordered Locus Names:	ESA_02237

Organism

Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	341 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves GlcNAc linked beta-1,4 to MurNAc tripeptides By similarity. HAMAP MF_00364
Catalytic activity	Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. HAMAP MF_00364
Pathway	Cell wall biogenesis; peptidoglycan recycling. HAMAP MF_00364
Subcellular location	Cytoplasm By similarity HAMAP MF_00364.
Sequence similarities	Belongs to the glycosyl hydrolase 3 family. NagZ subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-N-acetylhexosaminidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 341	341	Beta-hexosaminidase HAMAP MF_00364		PRO_1000005653
Sites
	Active site	248	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MFT1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: FC244DFB713E6EE3	FASTA	341	37,576
	10 20 30 40 50 60 MGPVMLDVEG FELDAEEREI LAHPLVGGLI LFTRNYHDPE QLRELVRQIR SSARNHLVIA 70 80 90 100 110 120 VDQEGGRVQR FREGFTRLPA AQSFAALLGM DAGGKLAEEA GWLMACEMIA MDIDISFAPV 130 140 150 160 170 180 LDVGHLSAAI GERSFHEEPE NVLAMAKRFI TGMQSAGMKA TGKHFPGHGA VSADSHKETP 190 200 210 220 230 240 RDPRPAAEIR RHDMLPFREL ISYNMLDAVM PAHVVYSDVD PLPASGSPYW LKQVLRKELG 250 260 270 280 290 300 FDGVIFSDDL SMEGAAILGS YAERGQAALD AGCDMILVCN NRKGAVSVLD NLSPIKAERV 310 320 330 340 TRLYHKGVIT RQELMASARW KQVSGELTAL HERWQTHKAG Q

References

[1]

"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species." Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J. PLoS ONE 5:E9556-E9556(2010) [PubMed: 20221447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-894.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000783 Genomic DNA. Translation: ABU77486.1.
RefSeq	YP_001438322.1. NC_009778.1.
3D structure databases
ProteinModelPortal	A7MFT1.
SMR	A7MFT1. Positions 1-334.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MFT1.
Protein family/group databases
CAZy	GH3. Glycoside Hydrolase Family 3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5551819.
GenomeReviews	Gene locus ESA_02237 in contig CP000783_GR.
KEGG	esa:ESA_02237.
PATRIC	20396421. VBICroSak107175_1998.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1472.
HOGENOM	HBG617255.
OMA	DLTMEGA.
ProtClustDB	PRK05337.
Family and domain databases
HAMAP	MF_00364. NagZ. [Tree]
InterPro	IPR022956. Beta_hexosaminidase. IPR019800. Glyco_hydro_3_AS. IPR001764. Glyco_hydro_3_N. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.300. Glyco_hydro_3_N. 1 hit.
KO	K01207.
Pfam	PF00933. Glyco_hydro_3. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00775. GLYCOSYL_HYDROL_F3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAGZ_CROS8

Accession

Primary (citable) accession number: A7MFT1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program