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A7MFQ4 (FABH_CROS8) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3

EC=2.3.1.180
Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III
Gene names
Name:fabH
Ordered Locus Names:ESA_02254
OrganismCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]
Taxonomic identifier290339 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP-Rule MF_01815

Catalytic activity

Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO2. HAMAP-Rule MF_01815

Pathway

Lipid metabolism; fatty acid biosynthesis. HAMAP-Rule MF_01815

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01815

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01815.

Domain

The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP-Rule MF_01815

Sequence similarities

Belongs to the FabH family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3173173-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP-Rule MF_01815
PRO_1000056361

Regions

Region245 – 2495ACP-binding By similarity

Sites

Active site1121 By similarity
Active site2441 By similarity
Active site2741 By similarity

Sequences

Sequence LengthMass (Da)Tools
A7MFQ4 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: C005F77BFCA15914

FASTA31733,539
        10         20         30         40         50         60 
MYTKIIGTGS YLPEHVRTNA DLEKMVETSD EWIVTRTGIR ERRIAAPGET VATMGYEAAL 

        70         80         90        100        110        120 
RAIDMAGIDK GQIGLIIVAT TSATHAFPSA ACQVQGMLGI KGCPAFDVAA ACAGFTYALS 

       130        140        150        160        170        180 
IADQYVKSGA VNNALVIGSD VLARTLDPED RGTIILFGDG AGAVVLGASE EPGILSTHLH 

       190        200        210        220        230        240 
ADGSYGELLT LPNQDRVNPD SPTFLTMAGN EVFKVAVTEL AHIVDETLEA NKLDRADLDW 

       250        260        270        280        290        300 
LVPHQANLRI ISATAKKLGM SMDNVVVTLD RHGNTSAASV PCALDEAVRD GRIQRGQLVL 

       310 
LEAFGGGFTW GSALVRF 

« Hide

References

[1]"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-894.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000783 Genomic DNA. Translation: ABU77503.1.
RefSeqYP_001438339.1. NC_009778.1.

3D structure databases

ProteinModelPortalA7MFQ4.
SMRA7MFQ4. Positions 1-317.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290339.ESA_02254.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU77503; ABU77503; ESA_02254.
GeneID5551744.
KEGGesa:ESA_02254.
PATRIC20396453. VBICroSak107175_2014.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0332.
HOGENOMHOG000246674.
KOK00648.
OMAIRIMQGT.
OrthoDBEOG6J74XN.
ProtClustDBPRK09352.

Enzyme and pathway databases

BioCycCSAK290339:GJ80-2245-MONOMER.
UniPathwayUPA00094.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
HAMAPMF_01815. FabH.
InterProIPR013751. ACP_syn_III.
IPR013747. ACP_syn_III_C.
IPR004655. FabH_synth.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF08545. ACP_syn_III. 1 hit.
PF08541. ACP_syn_III_C. 1 hit.
[Graphical view]
SUPFAMSSF53901. SSF53901. 1 hit.
TIGRFAMsTIGR00747. fabH. 1 hit.
ProtoNetSearch...

Entry information

Entry nameFABH_CROS8
AccessionPrimary (citable) accession number: A7MFQ4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: October 2, 2007
Last modified: February 19, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways