Reviewed, UniProtKB/Swiss-Prot A7MFA8 (BETA_ENTS8)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	ESA_02049

Organism

Enterobacter sakazakii (strain ATCC BAA-894) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Choline dehydrogenase HAMAP MF_00750		PRO_1000046563
Regions
	Nucleotide binding	4 – 33	30	FAD Probable
Sites
	Active site	473	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MFA8-1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 9429724C56A48C06	FASTA	559	62,376
	10 20 30 40 50 60 MEFDYIIIGA GSAGNVLATR LTEDSDVTVL LLEAGGPDYR FDFRTQMPAA LAYPLQGKRY 70 80 90 100 110 120 NWAYETEPEP YMNNRRMECG RGKGLGGSSL INGMCYIRGN AMDLDNWAQQ PGLERWTYLD 130 140 150 160 170 180 CLPYYRKSET RDIGANDYHG GDGPVSITTC KPGNNPLFAA MIEAGVQAGY PRTDDLNGYQ 190 200 210 220 230 240 QEGFGPMDRF VTPKGRRSST ARGYLDTAKQ RPNLKIITHA TTDRILFENK RAVGVAYLHG 250 260 270 280 290 300 ASNTPQEVHA RREVLLCAGA IASPQILQRS GVGNAELLKQ FDIPVVHDLP GVGENLQDHL 310 320 330 340 350 360 EMYLQYECKE PVSLYPALKW WNQPKIGAEW LFNGTGIGAS NHFEGGGFIR SREEFAWPNI 370 380 390 400 410 420 QYHFLPVAIN YNGSNAVEAH GFQCHVGSMR SPSRGHVRIK SRDPHQHPAI LFNYMSHEQD 430 440 450 460 470 480 WQEFRDAIRI TRQIINQPAL DKFRGREISP GIDCQTDEQL DEFVRNHAET AYHPCGTCKM 490 500 510 520 530 540 GSDEMAVVDD EGRVHGLEGL RVVDASIMPL IITGNLNATT IMIGEKIADN IRGRTPLPRS 550 TASYFVAGDR PVRGEPLRP

References

[1]	McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S., Fulton B., Wollam A., Shah N., Pepin K., Bhonagiri V., Nash W., Johnson M., Thiruvilangam P., Wilson R. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000783 Genomic DNA. Translation: ABU77300.1.
RefSeq	YP_001438136.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5549303.
GenomeReviews	Gene locus ESA_02049 in contig CP000783_GR.
KEGG	esa:ESA_02049.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A7MFA8. PNLQYHF.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_ENTS8

Accession

Primary (citable) accession number: A7MFA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	October 2, 2007
Last modified:	June 16, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)