A7MEZ1 (RLMF_CROS8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase F
EC=2.1.1.181

Alternative name(s):
23S rRNA mA1618 methyltransferase
rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	ESA_02533

Organism

Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]

Taxonomic identifier

290339 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Cronobacter

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity. HAMAP MF_01848
Catalytic activity	S-adenosyl-L-methionine + adenine(1618) in 23S rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine(1618) in 23S rRNA. HAMAP MF_01848
Subcellular location	Cytoplasm Potential HAMAP MF_01848.
Sequence similarities	Belongs to the methyltransferase superfamily. METTL16/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349903

Sequences

	Sequence		Length	Mass (Da)
	A7MEZ1 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 00A1FEC0566F4F5F	FASTA	309	34,000
	10 20 30 40 50 60 MNRKPGLHPR NRHHSRYDFD ALTESCPALG AFVRPSPAGE PTIDFADPQA VKTLNQALLA 70 80 90 100 110 120 HFYGVREWDI PDGFLCPPVP GRADYIHHLA DLLAEGNGGA LPAQASVLDI GVGANCIYPL 130 140 150 160 170 180 IGQHEYGWRF TGTDTSDEAI RSASAIIDAN PGLNRAIRLR RQKSPGAIFN GIIHKNESYD 190 200 210 220 230 240 ATLCNPPFHD SADAAEAGNA RKRRNLGLAA DSGLNFGGQQ QELWCEGGEV GFITQMIAES 250 260 270 280 290 300 KLFARQVLWF TTLVSKGDNL PLLYRALEQA GAVKVVKKEM AQGQKQSRFI AWSFLDTAQR ERWAQNRLR

References

[1]

"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species." Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J. PLoS ONE 5:E9556-E9556(2010) [PubMed: 20221447] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-894.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000783 Genomic DNA. Translation: ABU77778.1.
RefSeq	YP_001438614.1. NC_009778.1.
3D structure databases
ProteinModelPortal	A7MEZ1.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MEZ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5551598.
GenomeReviews	Gene locus ESA_02533 in contig CP000783_GR.
KEGG	esa:ESA_02533.
PATRIC	20396941. VBICroSak107175_2255.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3129.
HOGENOM	HBG747880.
OMA	DFANPEA.
ProtClustDB	PRK11727.
Family and domain databases
HAMAP	MF_01848. 23SrRNA_methyltr_F. [Tree]
InterPro	IPR016909. rRNA_lsu_methyltransferase_F. IPR010286. SAM-Mtase_prd. [Graphical view]
KO	K06970.
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_CROS8

Accession

Primary (citable) accession number: A7MEZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	October 2, 2007
Last modified:	January 25, 2012
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program