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A7MEE9

- OTSA_CROS8

UniProt

A7MEE9 - OTSA_CROS8

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Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor (By similarity).By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101Glucose-6-phosphateBy similarity
Binding sitei77 – 771Glucose-6-phosphateBy similarity
Sitei86 – 861Involved in alpha anomer selectivityBy similarity
Binding sitei131 – 1311Glucose-6-phosphateBy similarity
Sitei156 – 1561Involved in alpha anomer selectivityBy similarity
Binding sitei263 – 2631UDP-glucoseBy similarity
Binding sitei268 – 2681UDP-glucoseBy similarity
Binding sitei301 – 3011Glucose-6-phosphateBy similarity
Binding sitei340 – 3401UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

  1. alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity Source: UniProtKB-EC

GO - Biological processi

  1. trehalose biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-1330-MONOMER.
UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene namesi
Name:otsA
Ordered Locus Names:ESA_01335
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
ProteomesiUP000000260: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000348891Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi290339.ESA_01335.

Structurei

3D structure databases

ProteinModelPortaliA7MEE9.
SMRiA7MEE9. Positions 2-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 232UDP-glucose bindingBy similarity
Regioni366 – 3705UDP-glucose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.Curated

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191478.
KOiK00697.
OMAiWGESFVK.
OrthoDBiEOG6CCH1M.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

A7MEE9-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRLVVVSNR IAPPDDKKSS AGGLAVGILG ALKAAGGLWF GWSGEIGNED
60 70 80 90 100
APLKKVTRDN ITWASFNLSE QDHDQYYNQF SNGVLWPAFH YRLDLVNFQR
110 120 130 140 150
EAWEGYLRVN SLLADKLKPL IEPDDNLWIH DYHLLPFASE LRKRGVNNRI
160 170 180 190 200
GFFLHIPFPT PEIFNALPTN TELLEQLCDY DLLGFQTEND RTAFLDCLAM
210 220 230 240 250
QTHLSTSSDG EYTAYGKTFR TEVYPIGIEP EEIAQASAGP LPPKLAQLKA
260 270 280 290 300
ELASVKNIFS VERLDYSKGL PERFQAFETL LEKYPEHHGK IRYTQIAPTS
310 320 330 340 350
RGDVQAYQDI RHQLETEAGR INGKYGQLGW TPLYYLNQHF DRKLLMKVFR
360 370 380 390 400
YSDVGLVTPL RDGMNLVAKE YVAAQDPKNP GVLVLSQFAG AANELTAALL
410 420 430 440 450
VNPYDRDDVA AALDRALKMP LAERIARHSE MLEIVRKNDI NHWQEAFIKD
460 470
LKQVTPRSPE RELQNKVATF PKLA
Length:474
Mass (Da):53,673
Last modified:October 2, 2007 - v1
Checksum:iF209D0DD7320D9C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU76595.1.
RefSeqiYP_001437431.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU76595; ABU76595; ESA_01335.
GeneIDi5551440.
KEGGiesa:ESA_01335.
PATRICi20394777. VBICroSak107175_1180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU76595.1 .
RefSeqi YP_001437431.1. NC_009778.1.

3D structure databases

ProteinModelPortali A7MEE9.
SMRi A7MEE9. Positions 2-457.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 290339.ESA_01335.

Protein family/group databases

CAZyi GT20. Glycosyltransferase Family 20.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABU76595 ; ABU76595 ; ESA_01335 .
GeneIDi 5551440.
KEGGi esa:ESA_01335.
PATRICi 20394777. VBICroSak107175_1180.

Phylogenomic databases

eggNOGi COG0380.
HOGENOMi HOG000191478.
KOi K00697.
OMAi WGESFVK.
OrthoDBi EOG6CCH1M.

Enzyme and pathway databases

UniPathwayi UPA00299 .
BioCyci CSAK290339:GJ80-1330-MONOMER.

Family and domain databases

InterProi IPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view ]
Pfami PF00982. Glyco_transf_20. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR02400. trehalose_OtsA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiOTSA_CROS8
AccessioniPrimary (citable) accession number: A7MEE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 2, 2007
Last modified: November 26, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3