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Protein

Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

Gene

otsA

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor (By similarity).By similarity

Catalytic activityi

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathway:itrehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101Glucose-6-phosphateBy similarity
Binding sitei77 – 771Glucose-6-phosphateBy similarity
Sitei86 – 861Involved in alpha anomer selectivityBy similarity
Binding sitei131 – 1311Glucose-6-phosphateBy similarity
Sitei156 – 1561Involved in alpha anomer selectivityBy similarity
Binding sitei263 – 2631UDP-glucoseBy similarity
Binding sitei268 – 2681UDP-glucoseBy similarity
Binding sitei301 – 3011Glucose-6-phosphateBy similarity
Binding sitei340 – 3401UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciCSAK290339:GJ80-1330-MONOMER.
UniPathwayiUPA00299.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming] (EC:2.4.1.15)
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene namesi
Name:otsA
Ordered Locus Names:ESA_01335
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter
ProteomesiUP000000260 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Alpha,alpha-trehalose-phosphate synthase [UDP-forming]PRO_0000348891Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi290339.ESA_01335.

Structurei

3D structure databases

ProteinModelPortaliA7MEE9.
SMRiA7MEE9. Positions 2-457.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 232UDP-glucose bindingBy similarity
Regioni366 – 3705UDP-glucose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.Curated

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191478.
KOiK00697.
OMAiIEFMPIH.
OrthoDBiEOG6CCH1M.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.

Sequencei

Sequence statusi: Complete.

A7MEE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLVVVSNR IAPPDDKKSS AGGLAVGILG ALKAAGGLWF GWSGEIGNED
60 70 80 90 100
APLKKVTRDN ITWASFNLSE QDHDQYYNQF SNGVLWPAFH YRLDLVNFQR
110 120 130 140 150
EAWEGYLRVN SLLADKLKPL IEPDDNLWIH DYHLLPFASE LRKRGVNNRI
160 170 180 190 200
GFFLHIPFPT PEIFNALPTN TELLEQLCDY DLLGFQTEND RTAFLDCLAM
210 220 230 240 250
QTHLSTSSDG EYTAYGKTFR TEVYPIGIEP EEIAQASAGP LPPKLAQLKA
260 270 280 290 300
ELASVKNIFS VERLDYSKGL PERFQAFETL LEKYPEHHGK IRYTQIAPTS
310 320 330 340 350
RGDVQAYQDI RHQLETEAGR INGKYGQLGW TPLYYLNQHF DRKLLMKVFR
360 370 380 390 400
YSDVGLVTPL RDGMNLVAKE YVAAQDPKNP GVLVLSQFAG AANELTAALL
410 420 430 440 450
VNPYDRDDVA AALDRALKMP LAERIARHSE MLEIVRKNDI NHWQEAFIKD
460 470
LKQVTPRSPE RELQNKVATF PKLA
Length:474
Mass (Da):53,673
Last modified:October 2, 2007 - v1
Checksum:iF209D0DD7320D9C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU76595.1.
RefSeqiWP_012124438.1. NC_009778.1.

Genome annotation databases

EnsemblBacteriaiABU76595; ABU76595; ESA_01335.
GeneIDi5551440.
KEGGiesa:ESA_01335.
PATRICi20394777. VBICroSak107175_1180.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA. Translation: ABU76595.1.
RefSeqiWP_012124438.1. NC_009778.1.

3D structure databases

ProteinModelPortaliA7MEE9.
SMRiA7MEE9. Positions 2-457.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290339.ESA_01335.

Protein family/group databases

CAZyiGT20. Glycosyltransferase Family 20.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABU76595; ABU76595; ESA_01335.
GeneIDi5551440.
KEGGiesa:ESA_01335.
PATRICi20394777. VBICroSak107175_1180.

Phylogenomic databases

eggNOGiCOG0380.
HOGENOMiHOG000191478.
KOiK00697.
OMAiIEFMPIH.
OrthoDBiEOG6CCH1M.

Enzyme and pathway databases

UniPathwayiUPA00299.
BioCyciCSAK290339:GJ80-1330-MONOMER.

Family and domain databases

InterProiIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamiPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
    Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
    PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-894.

Entry informationi

Entry nameiOTSA_CROS8
AccessioniPrimary (citable) accession number: A7MEE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 2, 2007
Last modified: July 22, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.