Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Trehalose-6-phosphate synthase

Gene

otsA

Organism
Cronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Probably involved in the osmoprotection via the biosynthesis of trehalose. Catalyzes the transfer of glucose from UDP-alpha-D-glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor.By similarity

Catalytic activityi

UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.By similarity

Pathwayi: trehalose biosynthesis

This protein is involved in the pathway trehalose biosynthesis, which is part of Glycan biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway trehalose biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10D-glucose 6-phosphateBy similarity1
Binding sitei77D-glucose 6-phosphateBy similarity1
Sitei86Involved in alpha anomer selectivityBy similarity1
Binding sitei131D-glucose 6-phosphateBy similarity1
Sitei156Involved in alpha anomer selectivityBy similarity1
Binding sitei263UDP-glucoseBy similarity1
Binding sitei268UDP-glucoseBy similarity1
Binding sitei301D-glucose 6-phosphateBy similarity1
Binding sitei340UDP-glucose; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciCSAK290339:G1G9O-1261-MONOMER
UniPathwayiUPA00299

Protein family/group databases

CAZyiGT20 Glycosyltransferase Family 20

Names & Taxonomyi

Protein namesi
Recommended name:
Trehalose-6-phosphate synthaseBy similarity (EC:2.4.1.15By similarity)
Short name:
TPSBy similarity
Alternative name(s):
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]By similarity
Osmoregulatory trehalose synthesis protein ABy similarity
Short name:
OtsABy similarity
UDP-glucose-glucosephosphate glucosyltransferaseBy similarity
Gene namesi
Name:otsABy similarity
Ordered Locus Names:ESA_01335
OrganismiCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii)
Taxonomic identifieri290339 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeCronobacter
Proteomesi
  • UP000000260 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003488911 – 474Trehalose-6-phosphate synthaseAdd BLAST474

Proteomic databases

PRIDEiA7MEE9

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi290339.ESA_01335

Structurei

3D structure databases

ProteinModelPortaliA7MEE9
SMRiA7MEE9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni22 – 23UDP-glucose bindingBy similarity2
Regioni366 – 370UDP-glucose bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyltransferase 20 family.By similarity

Phylogenomic databases

eggNOGiENOG4105C1K Bacteria
COG0380 LUCA
HOGENOMiHOG000191478
KOiK00697
OMAiIEFMPIH

Family and domain databases

CDDicd03788 GT1_TPS, 1 hit
InterProiView protein in InterPro
IPR001830 Glyco_trans_20
IPR012766 Trehalose_OtsA
PfamiView protein in Pfam
PF00982 Glyco_transf_20, 1 hit
TIGRFAMsiTIGR02400 trehalose_OtsA, 1 hit

Sequencei

Sequence statusi: Complete.

A7MEE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRLVVVSNR IAPPDDKKSS AGGLAVGILG ALKAAGGLWF GWSGEIGNED
60 70 80 90 100
APLKKVTRDN ITWASFNLSE QDHDQYYNQF SNGVLWPAFH YRLDLVNFQR
110 120 130 140 150
EAWEGYLRVN SLLADKLKPL IEPDDNLWIH DYHLLPFASE LRKRGVNNRI
160 170 180 190 200
GFFLHIPFPT PEIFNALPTN TELLEQLCDY DLLGFQTEND RTAFLDCLAM
210 220 230 240 250
QTHLSTSSDG EYTAYGKTFR TEVYPIGIEP EEIAQASAGP LPPKLAQLKA
260 270 280 290 300
ELASVKNIFS VERLDYSKGL PERFQAFETL LEKYPEHHGK IRYTQIAPTS
310 320 330 340 350
RGDVQAYQDI RHQLETEAGR INGKYGQLGW TPLYYLNQHF DRKLLMKVFR
360 370 380 390 400
YSDVGLVTPL RDGMNLVAKE YVAAQDPKNP GVLVLSQFAG AANELTAALL
410 420 430 440 450
VNPYDRDDVA AALDRALKMP LAERIARHSE MLEIVRKNDI NHWQEAFIKD
460 470
LKQVTPRSPE RELQNKVATF PKLA
Length:474
Mass (Da):53,673
Last modified:October 2, 2007 - v1
Checksum:iF209D0DD7320D9C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000783 Genomic DNA Translation: ABU76595.1
RefSeqiWP_012124438.1, NC_009778.1

Genome annotation databases

EnsemblBacteriaiABU76595; ABU76595; ESA_01335
KEGGiesa:ESA_01335
PATRICifig|290339.8.peg.1180

Similar proteinsi

Entry informationi

Entry nameiOTSA_CROS8
AccessioniPrimary (citable) accession number: A7MEE9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 2, 2007
Last modified: May 23, 2018
This is version 60 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health