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A7MEE9 (OTSA_CROS8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha,alpha-trehalose-phosphate synthase [UDP-forming]

EC=2.4.1.15
Alternative name(s):
Osmoregulatory trehalose synthesis protein A
Trehalose-6-phosphate synthase
UDP-glucose-glucosephosphate glucosyltransferase
Gene names
Name:otsA
Ordered Locus Names:ESA_01335
OrganismCronobacter sakazakii (strain ATCC BAA-894) (Enterobacter sakazakii) [Complete proteome] [HAMAP]
Taxonomic identifier290339 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeCronobacter

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor By similarity.

Catalytic activity

UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate.

Pathway

Glycan biosynthesis; trehalose biosynthesis.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the glycosyltransferase 20 family.

Ontologies

Keywords
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtrehalose biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionalpha,alpha-trehalose-phosphate synthase (UDP-forming) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474Alpha,alpha-trehalose-phosphate synthase [UDP-forming]
PRO_0000348891

Regions

Region22 – 232UDP-glucose binding By similarity
Region366 – 3705UDP-glucose binding By similarity

Sites

Binding site101Glucose-6-phosphate By similarity
Binding site771Glucose-6-phosphate By similarity
Binding site1311Glucose-6-phosphate By similarity
Binding site2631UDP-glucose By similarity
Binding site2681UDP-glucose By similarity
Binding site3011Glucose-6-phosphate By similarity
Binding site3401UDP-glucose; via amide nitrogen and carbonyl oxygen By similarity
Site861Involved in alpha anomer selectivity By similarity
Site1561Involved in alpha anomer selectivity By similarity

Sequences

Sequence LengthMass (Da)Tools
A7MEE9 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: F209D0DD7320D9C7

FASTA47453,673
        10         20         30         40         50         60 
MSRLVVVSNR IAPPDDKKSS AGGLAVGILG ALKAAGGLWF GWSGEIGNED APLKKVTRDN 

        70         80         90        100        110        120 
ITWASFNLSE QDHDQYYNQF SNGVLWPAFH YRLDLVNFQR EAWEGYLRVN SLLADKLKPL 

       130        140        150        160        170        180 
IEPDDNLWIH DYHLLPFASE LRKRGVNNRI GFFLHIPFPT PEIFNALPTN TELLEQLCDY 

       190        200        210        220        230        240 
DLLGFQTEND RTAFLDCLAM QTHLSTSSDG EYTAYGKTFR TEVYPIGIEP EEIAQASAGP 

       250        260        270        280        290        300 
LPPKLAQLKA ELASVKNIFS VERLDYSKGL PERFQAFETL LEKYPEHHGK IRYTQIAPTS 

       310        320        330        340        350        360 
RGDVQAYQDI RHQLETEAGR INGKYGQLGW TPLYYLNQHF DRKLLMKVFR YSDVGLVTPL 

       370        380        390        400        410        420 
RDGMNLVAKE YVAAQDPKNP GVLVLSQFAG AANELTAALL VNPYDRDDVA AALDRALKMP 

       430        440        450        460        470 
LAERIARHSE MLEIVRKNDI NHWQEAFIKD LKQVTPRSPE RELQNKVATF PKLA 

« Hide

References

[1]"Genome sequence of Cronobacter sakazakii BAA-894 and comparative genomic hybridization analysis with other Cronobacter species."
Kucerova E., Clifton S.W., Xia X.Q., Long F., Porwollik S., Fulton L., Fronick C., Minx P., Kyung K., Warren W., Fulton R., Feng D., Wollam A., Shah N., Bhonagiri V., Nash W.E., Hallsworth-Pepin K., Wilson R.K., McClelland M., Forsythe S.J.
PLoS ONE 5:E9556-E9556(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-894.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000783 Genomic DNA. Translation: ABU76595.1.
RefSeqYP_001437431.1. NC_009778.1.

3D structure databases

ProteinModelPortalA7MEE9.
SMRA7MEE9. Positions 2-457.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290339.ESA_01335.

Protein family/group databases

CAZyGT20. Glycosyltransferase Family 20.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABU76595; ABU76595; ESA_01335.
GeneID5551440.
KEGGesa:ESA_01335.
PATRIC20394777. VBICroSak107175_1180.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0380.
HOGENOMHOG000191478.
KOK00697.
OMAWGESFVK.
OrthoDBEOG6CCH1M.

Enzyme and pathway databases

BioCycCSAK290339:GJ80-1330-MONOMER.
UniPathwayUPA00299.

Family and domain databases

InterProIPR001830. Glyco_trans_20.
IPR012766. Trehalose_OtsA.
[Graphical view]
PfamPF00982. Glyco_transf_20. 1 hit.
[Graphical view]
TIGRFAMsTIGR02400. trehalose_OtsA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameOTSA_CROS8
AccessionPrimary (citable) accession number: A7MEE9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: October 2, 2007
Last modified: May 14, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways