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A7MCY6 (TBKB1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
TANK-binding kinase 1-binding protein 1

Short name=TBK1-binding protein 1
Gene names
Name:TBKBP1
Synonyms:KIAA0775, SINTBAD
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length615 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Adapter protein which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity. Ref.8 UniProtKB A2A9T0

Subunit structure

Homodimer. May form a heterodimer with NAP1. Interacts with TKB1 and IKBKE. Ref.5 Ref.8 UniProtKB A2A9T0

Tissue specificity

Detected in leukocytes, lung, placenta, small intestine, liver, kidney, spleen, muscle, heart, brain and at low levels in thymus. Ref.5

Sequence caution

The sequence BAA34495.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processImmunity
Innate immunity
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processinnate immune response

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 Ref.3 (identifier: A7MCY6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.3 (identifier: A7MCY6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     186-222: PPAPAPPCTDLDLHYLALRGGSGLSHAGWPGSTPSVS → RRARAVLAVHQLADGDGGLRHPQLPPLPAGFPCRVPG
     223-615: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 615615TANK-binding kinase 1-binding protein 1
PRO_0000324654

Regions

Region1 – 279279Homodimerization By similarity
Region280 – 32950Interaction with TBK1 and IKBKE By similarity
Coiled coil48 – 162115 Potential
Coiled coil221 – 27656 Potential
Compositional bias343 – 43492Pro-rich

Amino acid modifications

Modified residue1841Phosphoserine Ref.7
Modified residue3651Phosphoserine Ref.7
Modified residue3721Phosphoserine Ref.7
Modified residue3791Phosphoserine Ref.7
Modified residue3851Phosphoserine Ref.7
Modified residue4001Phosphoserine Ref.7
Modified residue4151Phosphoserine Ref.7
Modified residue5041Phosphoserine Ref.7
Modified residue5341Phosphoserine Ref.7

Natural variations

Alternative sequence186 – 22237PPAPA…TPSVS → RRARAVLAVHQLADGDGGLR HPQLPPLPAGFPCRVPG in isoform 2. Ref.3
VSP_052714
Alternative sequence223 – 615393Missing in isoform 2. Ref.3
VSP_052715

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: C8F42919164A554E

FASTA61567,702
        10         20         30         40         50         60 
MESMFEDDIS ILTQEALGPS EVWLDSPGDP SLGGDMCSAS HFALITAYGD IKERLGGLER 

        70         80         90        100        110        120 
ENATLRRRLK VYEIKYPLIS DFGEEHGFSL YEIKDGSLLE VEKVSLQQRL NQFQHELQKN 

       130        140        150        160        170        180 
KEQEEQLGEM IQAYEKLCVE KSDLETELRE MRALVETHLR QICGLEQQLR QQQGLQDAAF 

       190        200        210        220        230        240 
SNLSPPPAPA PPCTDLDLHY LALRGGSGLS HAGWPGSTPS VSDLERRRLE EALEAAQGEA 

       250        260        270        280        290        300 
RGAQLREEQL QAECERLQGE LKQLQETRAQ DLASNQSERD MAWVKRVGDD QVNLALAYTE 

       310        320        330        340        350        360 
LTEELGRLRE LSSLQGRILR TLLQEQARSG GQRHSPLSQR HSPAPQCPSP SPPARAAPPC 

       370        380        390        400        410        420 
PPCQSPVPQR RSPVPPCPSP QQRRSPASPS CPSPVPQRRS PVPPSCQSPS PQRRSPVPPS 

       430        440        450        460        470        480 
CPAPQPRPPP PPPPGERTLA ERAYAKPPSH HVKAGFQGRR SYSELAEGAA YAGASPPWLQ 

       490        500        510        520        530        540 
AEAATLPKPR AYGSELYGPG RPLSPRRAFE GIRLRFEKQP SEEDEWAVPT SPPSPEVGTI 

       550        560        570        580        590        600 
RCASFCAGFP IPESPAATAY AHAEHAQSWP SINLLMETVG SDIRSCPLCQ LGFPVGYPDD 

       610 
ALIKHIDSHL ENSKI 

« Hide

Isoform 2 [UniParc].

Checksum: E52578DA01C2B322
Show »

FASTA22225,043

References

« Hide 'large scale' references
[1]"Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[2]Ohara O., Suyama M., Nagase T., Ishikawa K., Kikuno R.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
[4]"A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway."
Bouwmeester T., Bauch A., Ruffner H., Angrand P.-O., Bergamini G., Croughton K., Cruciat C., Eberhard D., Gagneur J., Ghidelli S., Hopf C., Huhse B., Mangano R., Michon A.-M., Schirle M., Schlegl J., Schwab M., Stein M.A. expand/collapse author list , Bauer A., Casari G., Drewes G., Gavin A.-C., Jackson D.B., Joberty G., Neubauer G., Rick J., Kuster B., Superti-Furga G.
Nat. Cell Biol. 6:97-105(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION.
[5]"SINTBAD, a novel component of innate antiviral immunity, shares a TBK1-binding domain with NAP1 and TANK."
Ryzhakov G., Randow F.
EMBO J. 26:3180-3190(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IKBKE, TISSUE SPECIFICITY.
[6]"Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184; SER-365; SER-372; SER-379; SER-385; SER-400; SER-415; SER-504 AND SER-534, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Functional dissection of the TBK1 molecular network."
Goncalves A., Burckstummer T., Dixit E., Scheicher R., Gorna M.W., Karayel E., Sugar C., Stukalov A., Berg T., Kralovics R., Planyavsky M., Bennett K.L., Colinge J., Superti-Furga G.
PLoS ONE 6:E23971-E23971(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH TBK1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB018318 mRNA. Translation: BAA34495.2. Different initiation.
BC111418 mRNA. Translation: AAI11419.1.
BC152407 mRNA. Translation: AAI52408.1.
BC167150 mRNA. Translation: AAI67150.1.
RefSeqNP_055541.1. NM_014726.2.
XP_005257916.1. XM_005257859.2.
XP_005257917.1. XM_005257860.2.
XP_005257918.1. XM_005257861.2.
XP_005257919.1. XM_005257862.2.
UniGeneHs.94790.

3D structure databases

ProteinModelPortalA7MCY6.
SMRA7MCY6. Positions 46-76.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid115103. 16 interactions.
DIPDIP-27604N.
IntActA7MCY6. 12 interactions.
MINTMINT-1141660.
STRING9606.ENSP00000354777.

PTM databases

PhosphoSiteA7MCY6.

Proteomic databases

PaxDbA7MCY6.
PRIDEA7MCY6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000361722; ENSP00000354777; ENSG00000198933. [A7MCY6-1]
GeneID9755.
KEGGhsa:9755.
UCSCuc002ilu.3. human. [A7MCY6-1]

Organism-specific databases

CTD9755.
GeneCardsGC17P045772.
HGNCHGNC:30140. TBKBP1.
MIM608476. gene.
neXtProtNX_A7MCY6.
PharmGKBPA142670830.
HUGESearch...
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG85276.
HOGENOMHOG000154514.
HOVERGENHBG108550.
InParanoidA7MCY6.
KOK12652.
OMAYGGELYG.
OrthoDBEOG7KQ21K.
PhylomeDBA7MCY6.
TreeFamTF331289.

Gene expression databases

ArrayExpressA7MCY6.
BgeeA7MCY6.
CleanExHS_TBKBP1.
GenevestigatorA7MCY6.

Family and domain databases

InterProIPR024581. TBD.
[Graphical view]
PfamPF12845. TBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi9755.
NextBio36720.
PROA7MCY6.
SOURCESearch...

Entry information

Entry nameTBKB1_HUMAN
AccessionPrimary (citable) accession number: A7MCY6
Secondary accession number(s): B2RZG6, O94873, Q14DW3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 2, 2007
Last modified: April 16, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 17

Human chromosome 17: entries, gene names and cross-references to MIM