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Protein

TANK-binding kinase 1-binding protein 1

Gene

TBKBP1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein which constitutively binds TBK1 and IKBKE playing a role in antiviral innate immunity.1 Publication

GO - Biological processi

  1. innate immune response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
TANK-binding kinase 1-binding protein 1
Short name:
TBK1-binding protein 1
Gene namesi
Name:TBKBP1Imported
Synonyms:KIAA0775Imported, SINTBAD
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 17

Organism-specific databases

HGNCiHGNC:30140. TBKBP1.

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142670830.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 615615TANK-binding kinase 1-binding protein 1PRO_0000324654Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei184 – 1841Phosphoserine1 Publication
Modified residuei365 – 3651Phosphoserine1 Publication
Modified residuei372 – 3721Phosphoserine1 Publication
Modified residuei379 – 3791Phosphoserine1 Publication
Modified residuei385 – 3851Phosphoserine1 Publication
Modified residuei400 – 4001Phosphoserine2 Publications
Modified residuei415 – 4151Phosphoserine1 Publication
Modified residuei504 – 5041Phosphoserine1 Publication
Modified residuei534 – 5341Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiA7MCY6.
PaxDbiA7MCY6.
PRIDEiA7MCY6.

PTM databases

PhosphoSiteiA7MCY6.

Expressioni

Tissue specificityi

Detected in leukocytes, lung, placenta, small intestine, liver, kidney, spleen, muscle, heart, brain and at low levels in thymus.1 Publication

Gene expression databases

BgeeiA7MCY6.
CleanExiHS_TBKBP1.
ExpressionAtlasiA7MCY6. baseline.
GenevestigatoriA7MCY6.

Organism-specific databases

HPAiHPA062347.
HPA064856.

Interactioni

Subunit structurei

Homodimer. May form a heterodimer with NAP1. Interacts with TKB1 and IKBKE.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AGO2Q9UKV82EBI-359969,EBI-528269
APBA3O960182EBI-359969,EBI-6115839
IKBKEQ141643EBI-359969,EBI-307369
RB1CC1Q8TDY22EBI-359969,EBI-1047793
TBK1Q9UHD27EBI-359969,EBI-356402

Protein-protein interaction databases

BioGridi115103. 17 interactions.
DIPiDIP-27604N.
IntActiA7MCY6. 12 interactions.
MINTiMINT-1141660.
STRINGi9606.ENSP00000354777.

Structurei

3D structure databases

ProteinModelPortaliA7MCY6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 279279HomodimerizationBy similarityAdd
BLAST
Regioni280 – 32950Interaction with TBK1 and IKBKEBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili48 – 162115Sequence AnalysisAdd
BLAST
Coiled coili221 – 27656Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi343 – 43492Pro-richSequence AnalysisAdd
BLAST

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiNOG85276.
GeneTreeiENSGT00390000008865.
HOGENOMiHOG000154514.
HOVERGENiHBG108550.
InParanoidiA7MCY6.
KOiK12652.
OMAiYGGELYG.
OrthoDBiEOG7KQ21K.
PhylomeDBiA7MCY6.
TreeFamiTF331289.

Family and domain databases

InterProiIPR024581. TBD.
[Graphical view]
PfamiPF12845. TBD. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 12 Publications (identifier: A7MCY6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MESMFEDDIS ILTQEALGPS EVWLDSPGDP SLGGDMCSAS HFALITAYGD
60 70 80 90 100
IKERLGGLER ENATLRRRLK VYEIKYPLIS DFGEEHGFSL YEIKDGSLLE
110 120 130 140 150
VEKVSLQQRL NQFQHELQKN KEQEEQLGEM IQAYEKLCVE KSDLETELRE
160 170 180 190 200
MRALVETHLR QICGLEQQLR QQQGLQDAAF SNLSPPPAPA PPCTDLDLHY
210 220 230 240 250
LALRGGSGLS HAGWPGSTPS VSDLERRRLE EALEAAQGEA RGAQLREEQL
260 270 280 290 300
QAECERLQGE LKQLQETRAQ DLASNQSERD MAWVKRVGDD QVNLALAYTE
310 320 330 340 350
LTEELGRLRE LSSLQGRILR TLLQEQARSG GQRHSPLSQR HSPAPQCPSP
360 370 380 390 400
SPPARAAPPC PPCQSPVPQR RSPVPPCPSP QQRRSPASPS CPSPVPQRRS
410 420 430 440 450
PVPPSCQSPS PQRRSPVPPS CPAPQPRPPP PPPPGERTLA ERAYAKPPSH
460 470 480 490 500
HVKAGFQGRR SYSELAEGAA YAGASPPWLQ AEAATLPKPR AYGSELYGPG
510 520 530 540 550
RPLSPRRAFE GIRLRFEKQP SEEDEWAVPT SPPSPEVGTI RCASFCAGFP
560 570 580 590 600
IPESPAATAY AHAEHAQSWP SINLLMETVG SDIRSCPLCQ LGFPVGYPDD
610
ALIKHIDSHL ENSKI
Length:615
Mass (Da):67,702
Last modified:October 2, 2007 - v1
Checksum:iC8F42919164A554E
GO
Isoform 21 Publication (identifier: A7MCY6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     186-222: PPAPAPPCTDLDLHYLALRGGSGLSHAGWPGSTPSVS → RRARAVLAVHQLADGDGGLRHPQLPPLPAGFPCRVPG
     223-615: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:222
Mass (Da):25,043
Checksum:iE52578DA01C2B322
GO

Sequence cautioni

The sequence BAA34495.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei186 – 22237PPAPA…TPSVS → RRARAVLAVHQLADGDGGLR HPQLPPLPAGFPCRVPG in isoform 2. 1 PublicationVSP_052714Add
BLAST
Alternative sequencei223 – 615393Missing in isoform 2. 1 PublicationVSP_052715Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018318 mRNA. Translation: BAA34495.2. Different initiation.
BC111418 mRNA. Translation: AAI11419.1.
BC152407 mRNA. Translation: AAI52408.1.
BC167150 mRNA. Translation: AAI67150.1.
CCDSiCCDS45722.1. [A7MCY6-1]
RefSeqiNP_055541.1. NM_014726.2. [A7MCY6-1]
XP_005257916.1. XM_005257859.2. [A7MCY6-1]
XP_005257917.1. XM_005257860.2. [A7MCY6-1]
XP_005257918.1. XM_005257861.2. [A7MCY6-1]
XP_005257919.1. XM_005257862.2. [A7MCY6-1]
UniGeneiHs.94790.

Genome annotation databases

EnsembliENST00000361722; ENSP00000354777; ENSG00000198933. [A7MCY6-1]
ENST00000622396; ENSP00000484395; ENSG00000198933. [A7MCY6-2]
GeneIDi9755.
KEGGihsa:9755.
UCSCiuc002ilu.3. human. [A7MCY6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB018318 mRNA. Translation: BAA34495.2. Different initiation.
BC111418 mRNA. Translation: AAI11419.1.
BC152407 mRNA. Translation: AAI52408.1.
BC167150 mRNA. Translation: AAI67150.1.
CCDSiCCDS45722.1. [A7MCY6-1]
RefSeqiNP_055541.1. NM_014726.2. [A7MCY6-1]
XP_005257916.1. XM_005257859.2. [A7MCY6-1]
XP_005257917.1. XM_005257860.2. [A7MCY6-1]
XP_005257918.1. XM_005257861.2. [A7MCY6-1]
XP_005257919.1. XM_005257862.2. [A7MCY6-1]
UniGeneiHs.94790.

3D structure databases

ProteinModelPortaliA7MCY6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115103. 17 interactions.
DIPiDIP-27604N.
IntActiA7MCY6. 12 interactions.
MINTiMINT-1141660.
STRINGi9606.ENSP00000354777.

PTM databases

PhosphoSiteiA7MCY6.

Proteomic databases

MaxQBiA7MCY6.
PaxDbiA7MCY6.
PRIDEiA7MCY6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000361722; ENSP00000354777; ENSG00000198933. [A7MCY6-1]
ENST00000622396; ENSP00000484395; ENSG00000198933. [A7MCY6-2]
GeneIDi9755.
KEGGihsa:9755.
UCSCiuc002ilu.3. human. [A7MCY6-1]

Organism-specific databases

CTDi9755.
GeneCardsiGC17P045772.
HGNCiHGNC:30140. TBKBP1.
HPAiHPA062347.
HPA064856.
MIMi608476. gene.
neXtProtiNX_A7MCY6.
PharmGKBiPA142670830.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG85276.
GeneTreeiENSGT00390000008865.
HOGENOMiHOG000154514.
HOVERGENiHBG108550.
InParanoidiA7MCY6.
KOiK12652.
OMAiYGGELYG.
OrthoDBiEOG7KQ21K.
PhylomeDBiA7MCY6.
TreeFamiTF331289.

Miscellaneous databases

GenomeRNAii9755.
NextBioi36720.
PROiA7MCY6.
SOURCEiSearch...

Gene expression databases

BgeeiA7MCY6.
CleanExiHS_TBKBP1.
ExpressionAtlasiA7MCY6. baseline.
GenevestigatoriA7MCY6.

Family and domain databases

InterProiIPR024581. TBD.
[Graphical view]
PfamiPF12845. TBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro."
    Nagase T., Ishikawa K., Suyama M., Kikuno R., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.
    DNA Res. 5:277-286(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: BrainImported.
  2. Ohara O., Suyama M., Nagase T., Ishikawa K., Kikuno R.
    Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
  4. Cited for: IDENTIFICATION.
  5. "SINTBAD, a novel component of innate antiviral immunity, shares a TBK1-binding domain with NAP1 and TANK."
    Ryzhakov G., Randow F.
    EMBO J. 26:3180-3190(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IKBKE, TISSUE SPECIFICITY.
  6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-184; SER-365; SER-372; SER-379; SER-385; SER-400; SER-415; SER-504 AND SER-534, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: FUNCTION, INTERACTION WITH TBK1.
  9. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-400, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiTBKB1_HUMAN
AccessioniPrimary (citable) accession number: A7MCY6
Secondary accession number(s): B2RZG6, O94873, Q14DW3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: October 2, 2007
Last modified: March 4, 2015
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.