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A7MCT6 (EKI2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ethanolamine kinase 2
Short name=EKI 2
EC=2.7.1.82
Alternative name(s):
Ethanolamine kinase-like protein
Gene names
Name:Etnk2
Synonyms:Tuc1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Highly specific for ethanolamine phosphorylation. Does not have choline kinase activity. Ref.4

Catalytic activity

ATP + ethanolamine = ADP + O-phosphoethanolamine. Ref.4

Pathway

Phospholipid metabolism; phosphatidylethanolamine biosynthesis; phosphatidylethanolamine from ethanolamine: step 1/3.

Tissue specificity

Expressed in testis and liver. Low expression in ovary and kidney. Ref.3 Ref.4 Ref.5

Developmental stage

The expression is restricted to the gonads during the sex determination period and throughout embryogenesis. In developing testis, the expression is found only in the Sertoli cells. The expression is strongest at day 11.5, more intense in the testis than the ovary. From 12.5 dpc the expression in the ovary is reduced and disappears. The expression at day 13.5 dpc is restricted to the testis cords. Ref.3

Disruption phenotype

No visible phenotype in adults. No significant effect is seen on liver phospholipid metabolism, neural development, or testicular function. No abnormalities are detected in embryonic and adult testis morphology, differentiation, function, or fertility. Ref.4 shows maternal-specific failure to support late embryonic development, resulting in reduced perinatal size and survival and suggesting compromised placental function. Ref.4 Ref.5

Sequence similarities

Belongs to the choline/ethanolamine kinase family.

Caution

According to Ref.4, disruption of the gene causes increased prevalence of placental thrombosis, reduced litter size and increased pup mortality. No such effect was observed by Ref.5. One possible explanation lies in the fact that Ref.4 observed a remarkable 100% survival of control pups and 16% mortality for mutant pups, while Ref.5 observed 16% mortality for both wild-type and mutant pups. According to Ref.5, the construct used in Ref.4 may lead to expression of a truncated transcript that might have deleterious effects.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: A7MCT6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A7MCT6-2)

The sequence of this isoform differs from the canonical sequence as follows:
     290-385: VNVVDYSRYP...PQVSALEMPK → RGGLL

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 385385Ethanolamine kinase 2
PRO_0000393962

Natural variations

Alternative sequence290 – 38596VNVVD…LEMPK → RGGLL in isoform 2.
VSP_039099

Experimental info

Sequence conflict1661R → Q in AAP47267. Ref.3
Sequence conflict2921V → E in AAP47267. Ref.3
Sequence conflict3361A → S in AAP47267. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 46C062858F7FFD29

FASTA38544,567
        10         20         30         40         50         60 
MAVPPSAPVP CSPFYLRRQE PCPQCSWSME EKAVASAGCW EPPGPPRAAV PCFSVTVEQD 

        70         80         90        100        110        120 
DILPGALRLI RELRPHWKPE QVRTKRFKDG ITNKLLACYV EEDMRDCVLV RVYGERTELL 

       130        140        150        160        170        180 
VDRENEVRNF QLLRAHGCAP KLYCTFQNGL CYEYVQGVAL GPEHIREPQL FRLIALEMAK 

       190        200        210        220        230        240 
IHTIHANGSL PKPTLWHKMH RYFTLVKDEI SPSLSADVPK VEVLEQELAW LKEHLSQLDS 

       250        260        270        280        290        300 
PVVFCHNDLL CKNIIYDSDK GRVCFIDYEY AGYNYQAFDI GNHFNEFAGV NVVDYSRYPA 

       310        320        330        340        350        360 
RETQVQWLRY YLEAQKGTAA SPREVERLYA QVNKFALASH FFWALWALIQ NQYSTISFDF 

       370        380 
LRYAVIRFNQ YFKVKPQVSA LEMPK

« Hide

Isoform 2 [UniParc].

Checksum: 3076342DA02B10CF
Show »

FASTA29433,620

References

« Hide 'large scale' references
[1]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-385 (ISOFORM 2).
Tissue: Brain.
[3]"Eki2 is upregulated specifically in the testis during mouse sex determination."
Hurley T.M., McClive P.J., Sarraj M.A., Sinclair A.H.
Gene Expr. Patterns 4:135-140(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 74-385 (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: C3H X 101.
[4]"Placental thrombosis and spontaneous fetal death in mice deficient in ethanolamine kinase 2."
Tian Y., Jackson P., Gunter C., Wang J., Rock C.O., Jackowski S.
J. Biol. Chem. 281:28438-28449(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
[5]"Testis development, fertility, and survival in ethanolamine kinase 2-deficient mice."
Gustin S.E., Western P.S., McClive P.J., Harley V.R., Koopman P.A., Sinclair A.H.
Endocrinology 149:6176-6186(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC068906 Genomic DNA. No translation available.
BC145351 mRNA. Translation: AAI45352.1.
BC152310 mRNA. Translation: AAI52311.1.
AY212244 mRNA. Translation: AAP47267.1.
IPIIPI00453708.
IPI00968343.
RefSeqNP_780652.2. NM_175443.5.
UniGeneMm.52111.

3D structure databases

ProteinModelPortalA7MCT6.
SMRA7MCT6. Positions 78-380.
ModBaseSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000114272.

Proteomic databases

PaxDbA7MCT6.
PRIDEA7MCT6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000135222; ENSMUSP00000114272; ENSMUSG00000070644.
GeneID214253.
KEGGmmu:214253.
UCSCuc007cqg.2. mouse.
uc011wsa.2. mouse.

Organism-specific databases

CTD55224.
MGIMGI:2443760. Etnk2.

Phylogenomic databases

eggNOGCOG0510.
GeneTreeENSGT00530000062991.
HOGENOMHOG000004856.
HOVERGENHBG018981.
InParanoidQ6XRG2.
KOK00894.
OMAGIDEIDY.
OrthoDBEOG4HT8S7.

Enzyme and pathway databases

UniPathwayUPA00558; UER00741.

Gene expression databases

ArrayExpressA7MCT6.
BgeeA7MCT6.
GenevestigatorA7MCT6.

Family and domain databases

InterProIPR026712. Cho/Etha_kinase.
IPR011009. Kinase-like_dom.
[Graphical view]
PANTHERPTHR22603. PTHR22603. 1 hit.
SUPFAMSSF56112. Kinase_like. 1 hit.
ProtoNetSearch...

Other

NextBio374240.
SOURCESearch...

Entry information

Entry nameEKI2_MOUSE
AccessionPrimary (citable) accession number: A7MCT6
Secondary accession number(s): B7ZNN6, D3Z771, Q6XRG2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: October 2, 2007
Last modified: May 29, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents