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Protein

Ethanolamine kinase 2

Gene

Etnk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Highly specific for ethanolamine phosphorylation. Does not have choline kinase activity.1 Publication

Catalytic activityi

ATP + ethanolamine = ADP + O-phosphoethanolamine.1 Publication

Pathway: phosphatidylethanolamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes phosphatidylethanolamine from ethanolamine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Choline/ethanolamine kinase (Chkb), Ethanolamine kinase 2 (Etnk2), Ethanolamine kinase 1 (Etnk1), Choline kinase alpha (Chka)
  2. Ethanolamine-phosphate cytidylyltransferase (Pcyt2)
  3. Choline/ethanolaminephosphotransferase 1 (Cept1), Ethanolaminephosphotransferase 1 (Ept1)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from ethanolamine, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ethanolamine kinase activity Source: UniProtKB

GO - Biological processi

  • in utero embryonic development Source: MGI
  • multicellular organism growth Source: MGI
  • phosphatidylethanolamine biosynthetic process Source: MGI
  • phosphorylation Source: UniProtKB
  • placenta development Source: MGI
  • post-embryonic development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.82. 3474.
ReactomeiREACT_354254. Synthesis of PE.
UniPathwayiUPA00558; UER00741.

Names & Taxonomyi

Protein namesi
Recommended name:
Ethanolamine kinase 2 (EC:2.7.1.82)
Short name:
EKI 2
Alternative name(s):
Ethanolamine kinase-like protein
Gene namesi
Name:Etnk2
Synonyms:Tuc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2443760. Etnk2.

Pathology & Biotechi

Disruption phenotypei

No visible phenotype in adults. No significant effect is seen on liver phospholipid metabolism, neural development, or testicular function. No abnormalities are detected in embryonic and adult testis morphology, differentiation, function, or fertility. PubMed:16861741 shows maternal-specific failure to support late embryonic development, resulting in reduced perinatal size and survival and suggesting compromised placental function.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385Ethanolamine kinase 2PRO_0000393962Add
BLAST

Proteomic databases

MaxQBiA7MCT6.
PaxDbiA7MCT6.
PRIDEiA7MCT6.

Expressioni

Tissue specificityi

Expressed in testis and liver. Low expression in ovary and kidney.3 Publications

Developmental stagei

The expression is restricted to the gonads during the sex determination period and throughout embryogenesis. In developing testis, the expression is found only in the Sertoli cells. The expression is strongest at day 11.5, more intense in the testis than the ovary. From 12.5 dpc the expression in the ovary is reduced and disappears. The expression at day 13.5 dpc is restricted to the testis cords.1 Publication

Gene expression databases

BgeeiA7MCT6.
ExpressionAtlasiA7MCT6. baseline and differential.
GenevisibleiA7MCT6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000114272.

Structurei

3D structure databases

ProteinModelPortaliA7MCT6.
SMRiA7MCT6. Positions 78-380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the choline/ethanolamine kinase family.Curated

Phylogenomic databases

eggNOGiCOG0510.
GeneTreeiENSGT00530000062991.
HOGENOMiHOG000004856.
HOVERGENiHBG018981.
InParanoidiA7MCT6.
KOiK00894.
OMAiKPMPMIW.
OrthoDBiEOG72NRQH.
PhylomeDBiA7MCT6.
TreeFamiTF313549.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A7MCT6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAVPPSAPVP CSPFYLRRQE PCPQCSWSME EKAVASAGCW EPPGPPRAAV
60 70 80 90 100
PCFSVTVEQD DILPGALRLI RELRPHWKPE QVRTKRFKDG ITNKLLACYV
110 120 130 140 150
EEDMRDCVLV RVYGERTELL VDRENEVRNF QLLRAHGCAP KLYCTFQNGL
160 170 180 190 200
CYEYVQGVAL GPEHIREPQL FRLIALEMAK IHTIHANGSL PKPTLWHKMH
210 220 230 240 250
RYFTLVKDEI SPSLSADVPK VEVLEQELAW LKEHLSQLDS PVVFCHNDLL
260 270 280 290 300
CKNIIYDSDK GRVCFIDYEY AGYNYQAFDI GNHFNEFAGV NVVDYSRYPA
310 320 330 340 350
RETQVQWLRY YLEAQKGTAA SPREVERLYA QVNKFALASH FFWALWALIQ
360 370 380
NQYSTISFDF LRYAVIRFNQ YFKVKPQVSA LEMPK
Length:385
Mass (Da):44,567
Last modified:October 2, 2007 - v1
Checksum:i46C062858F7FFD29
GO
Isoform 2 (identifier: A7MCT6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     290-385: VNVVDYSRYP...PQVSALEMPK → RGGLL

Show »
Length:294
Mass (Da):33,620
Checksum:i3076342DA02B10CF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti166 – 1661R → Q in AAP47267 (PubMed:15161093).Curated
Sequence conflicti292 – 2921V → E in AAP47267 (PubMed:15161093).Curated
Sequence conflicti336 – 3361A → S in AAP47267 (PubMed:15161093).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei290 – 38596VNVVD…LEMPK → RGGLL in isoform 2. 1 PublicationVSP_039099Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068906 Genomic DNA. No translation available.
BC145351 mRNA. Translation: AAI45352.1.
BC152310 mRNA. Translation: AAI52311.1.
AY212244 mRNA. Translation: AAP47267.1.
CCDSiCCDS15296.2. [A7MCT6-1]
RefSeqiNP_780652.2. NM_175443.5. [A7MCT6-1]
UniGeneiMm.52111.

Genome annotation databases

EnsembliENSMUST00000135222; ENSMUSP00000114272; ENSMUSG00000070644. [A7MCT6-1]
GeneIDi214253.
KEGGimmu:214253.
UCSCiuc007cqg.2. mouse. [A7MCT6-1]
uc011wsa.2. mouse. [A7MCT6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC068906 Genomic DNA. No translation available.
BC145351 mRNA. Translation: AAI45352.1.
BC152310 mRNA. Translation: AAI52311.1.
AY212244 mRNA. Translation: AAP47267.1.
CCDSiCCDS15296.2. [A7MCT6-1]
RefSeqiNP_780652.2. NM_175443.5. [A7MCT6-1]
UniGeneiMm.52111.

3D structure databases

ProteinModelPortaliA7MCT6.
SMRiA7MCT6. Positions 78-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000114272.

Proteomic databases

MaxQBiA7MCT6.
PaxDbiA7MCT6.
PRIDEiA7MCT6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000135222; ENSMUSP00000114272; ENSMUSG00000070644. [A7MCT6-1]
GeneIDi214253.
KEGGimmu:214253.
UCSCiuc007cqg.2. mouse. [A7MCT6-1]
uc011wsa.2. mouse. [A7MCT6-2]

Organism-specific databases

CTDi55224.
MGIiMGI:2443760. Etnk2.

Phylogenomic databases

eggNOGiCOG0510.
GeneTreeiENSGT00530000062991.
HOGENOMiHOG000004856.
HOVERGENiHBG018981.
InParanoidiA7MCT6.
KOiK00894.
OMAiKPMPMIW.
OrthoDBiEOG72NRQH.
PhylomeDBiA7MCT6.
TreeFamiTF313549.

Enzyme and pathway databases

UniPathwayiUPA00558; UER00741.
BRENDAi2.7.1.82. 3474.
ReactomeiREACT_354254. Synthesis of PE.

Miscellaneous databases

NextBioi374240.
PROiA7MCT6.
SOURCEiSearch...

Gene expression databases

BgeeiA7MCT6.
ExpressionAtlasiA7MCT6. baseline and differential.
GenevisibleiA7MCT6. MM.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-385 (ISOFORM 2).
    Tissue: Brain.
  3. "Eki2 is upregulated specifically in the testis during mouse sex determination."
    Hurley T.M., McClive P.J., Sarraj M.A., Sinclair A.H.
    Gene Expr. Patterns 4:135-140(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 74-385 (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: C3H X 101.
  4. "Placental thrombosis and spontaneous fetal death in mice deficient in ethanolamine kinase 2."
    Tian Y., Jackson P., Gunter C., Wang J., Rock C.O., Jackowski S.
    J. Biol. Chem. 281:28438-28449(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
  5. "Testis development, fertility, and survival in ethanolamine kinase 2-deficient mice."
    Gustin S.E., Western P.S., McClive P.J., Harley V.R., Koopman P.A., Sinclair A.H.
    Endocrinology 149:6176-6186(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiEKI2_MOUSE
AccessioniPrimary (citable) accession number: A7MCT6
Secondary accession number(s): B7ZNN6, D3Z771, Q6XRG2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 18, 2010
Last sequence update: October 2, 2007
Last modified: June 24, 2015
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

According to PubMed:16861741, disruption of the gene causes increased prevalence of placental thrombosis, reduced litter size and increased pup mortality. No such effect was observed by PubMed:18755794. One possible explanation lies in the fact that PubMed:16861741 observed a remarkable 100% survival of control pups and 16% mortality for mutant pups, while PubMed:18755794 observed 16% mortality for both wild-type and mutant pups. According to PubMed:18755794, the construct used in PubMed:16861741 may lead to expression of a truncated transcript that might have deleterious effects.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.