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A7MCT6

- EKI2_MOUSE

UniProt

A7MCT6 - EKI2_MOUSE

Protein

Ethanolamine kinase 2

Gene

Etnk2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Highly specific for ethanolamine phosphorylation. Does not have choline kinase activity.1 Publication

    Catalytic activityi

    ATP + ethanolamine = ADP + O-phosphoethanolamine.1 Publication

    Pathwayi

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ethanolamine kinase activity Source: UniProtKB

    GO - Biological processi

    1. in utero embryonic development Source: MGI
    2. multicellular organism growth Source: MGI
    3. phosphatidylethanolamine biosynthetic process Source: MGI
    4. phosphorylation Source: UniProtKB
    5. placenta development Source: MGI
    6. post-embryonic development Source: MGI

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_199009. Synthesis of PE.
    UniPathwayiUPA00558; UER00741.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ethanolamine kinase 2 (EC:2.7.1.82)
    Short name:
    EKI 2
    Alternative name(s):
    Ethanolamine kinase-like protein
    Gene namesi
    Name:Etnk2
    Synonyms:Tuc1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2443760. Etnk2.

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype in adults. No significant effect is seen on liver phospholipid metabolism, neural development, or testicular function. No abnormalities are detected in embryonic and adult testis morphology, differentiation, function, or fertility. PubMed:16861741 shows maternal-specific failure to support late embryonic development, resulting in reduced perinatal size and survival and suggesting compromised placental function.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 385385Ethanolamine kinase 2PRO_0000393962Add
    BLAST

    Proteomic databases

    MaxQBiA7MCT6.
    PaxDbiA7MCT6.
    PRIDEiA7MCT6.

    Expressioni

    Tissue specificityi

    Expressed in testis and liver. Low expression in ovary and kidney.3 Publications

    Developmental stagei

    The expression is restricted to the gonads during the sex determination period and throughout embryogenesis. In developing testis, the expression is found only in the Sertoli cells. The expression is strongest at day 11.5, more intense in the testis than the ovary. From 12.5 dpc the expression in the ovary is reduced and disappears. The expression at day 13.5 dpc is restricted to the testis cords.1 Publication

    Gene expression databases

    BgeeiA7MCT6.
    GenevestigatoriA7MCT6.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000114272.

    Structurei

    3D structure databases

    ProteinModelPortaliA7MCT6.
    SMRiA7MCT6. Positions 78-380.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the choline/ethanolamine kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0510.
    GeneTreeiENSGT00530000062991.
    HOGENOMiHOG000004856.
    HOVERGENiHBG018981.
    InParanoidiQ6XRG2.
    KOiK00894.
    OMAiQNQFSTI.
    OrthoDBiEOG72NRQH.
    PhylomeDBiA7MCT6.
    TreeFamiTF313549.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: A7MCT6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAVPPSAPVP CSPFYLRRQE PCPQCSWSME EKAVASAGCW EPPGPPRAAV    50
    PCFSVTVEQD DILPGALRLI RELRPHWKPE QVRTKRFKDG ITNKLLACYV 100
    EEDMRDCVLV RVYGERTELL VDRENEVRNF QLLRAHGCAP KLYCTFQNGL 150
    CYEYVQGVAL GPEHIREPQL FRLIALEMAK IHTIHANGSL PKPTLWHKMH 200
    RYFTLVKDEI SPSLSADVPK VEVLEQELAW LKEHLSQLDS PVVFCHNDLL 250
    CKNIIYDSDK GRVCFIDYEY AGYNYQAFDI GNHFNEFAGV NVVDYSRYPA 300
    RETQVQWLRY YLEAQKGTAA SPREVERLYA QVNKFALASH FFWALWALIQ 350
    NQYSTISFDF LRYAVIRFNQ YFKVKPQVSA LEMPK 385
    Length:385
    Mass (Da):44,567
    Last modified:October 2, 2007 - v1
    Checksum:i46C062858F7FFD29
    GO
    Isoform 2 (identifier: A7MCT6-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         290-385: VNVVDYSRYP...PQVSALEMPK → RGGLL

    Show »
    Length:294
    Mass (Da):33,620
    Checksum:i3076342DA02B10CF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti166 – 1661R → Q in AAP47267. (PubMed:15161093)Curated
    Sequence conflicti292 – 2921V → E in AAP47267. (PubMed:15161093)Curated
    Sequence conflicti336 – 3361A → S in AAP47267. (PubMed:15161093)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei290 – 38596VNVVD…LEMPK → RGGLL in isoform 2. 1 PublicationVSP_039099Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC068906 Genomic DNA. No translation available.
    BC145351 mRNA. Translation: AAI45352.1.
    BC152310 mRNA. Translation: AAI52311.1.
    AY212244 mRNA. Translation: AAP47267.1.
    CCDSiCCDS15296.2. [A7MCT6-1]
    RefSeqiNP_780652.2. NM_175443.5. [A7MCT6-1]
    UniGeneiMm.52111.

    Genome annotation databases

    EnsembliENSMUST00000135222; ENSMUSP00000114272; ENSMUSG00000070644. [A7MCT6-1]
    GeneIDi214253.
    KEGGimmu:214253.
    UCSCiuc007cqg.2. mouse. [A7MCT6-1]
    uc011wsa.2. mouse. [A7MCT6-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC068906 Genomic DNA. No translation available.
    BC145351 mRNA. Translation: AAI45352.1 .
    BC152310 mRNA. Translation: AAI52311.1 .
    AY212244 mRNA. Translation: AAP47267.1 .
    CCDSi CCDS15296.2. [A7MCT6-1 ]
    RefSeqi NP_780652.2. NM_175443.5. [A7MCT6-1 ]
    UniGenei Mm.52111.

    3D structure databases

    ProteinModelPortali A7MCT6.
    SMRi A7MCT6. Positions 78-380.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000114272.

    Proteomic databases

    MaxQBi A7MCT6.
    PaxDbi A7MCT6.
    PRIDEi A7MCT6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000135222 ; ENSMUSP00000114272 ; ENSMUSG00000070644 . [A7MCT6-1 ]
    GeneIDi 214253.
    KEGGi mmu:214253.
    UCSCi uc007cqg.2. mouse. [A7MCT6-1 ]
    uc011wsa.2. mouse. [A7MCT6-2 ]

    Organism-specific databases

    CTDi 55224.
    MGIi MGI:2443760. Etnk2.

    Phylogenomic databases

    eggNOGi COG0510.
    GeneTreei ENSGT00530000062991.
    HOGENOMi HOG000004856.
    HOVERGENi HBG018981.
    InParanoidi Q6XRG2.
    KOi K00894.
    OMAi QNQFSTI.
    OrthoDBi EOG72NRQH.
    PhylomeDBi A7MCT6.
    TreeFami TF313549.

    Enzyme and pathway databases

    UniPathwayi UPA00558 ; UER00741 .
    Reactomei REACT_199009. Synthesis of PE.

    Miscellaneous databases

    NextBioi 374240.
    PROi A7MCT6.
    SOURCEi Search...

    Gene expression databases

    Bgeei A7MCT6.
    Genevestigatori A7MCT6.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 11-385 (ISOFORM 2).
      Tissue: Brain.
    3. "Eki2 is upregulated specifically in the testis during mouse sex determination."
      Hurley T.M., McClive P.J., Sarraj M.A., Sinclair A.H.
      Gene Expr. Patterns 4:135-140(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 74-385 (ISOFORM 1), TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: C3H X 101.
    4. "Placental thrombosis and spontaneous fetal death in mice deficient in ethanolamine kinase 2."
      Tian Y., Jackson P., Gunter C., Wang J., Rock C.O., Jackowski S.
      J. Biol. Chem. 281:28438-28449(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.
    5. "Testis development, fertility, and survival in ethanolamine kinase 2-deficient mice."
      Gustin S.E., Western P.S., McClive P.J., Harley V.R., Koopman P.A., Sinclair A.H.
      Endocrinology 149:6176-6186(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiEKI2_MOUSE
    AccessioniPrimary (citable) accession number: A7MCT6
    Secondary accession number(s): B7ZNN6, D3Z771, Q6XRG2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 18, 2010
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    According to PubMed:16861741, disruption of the gene causes increased prevalence of placental thrombosis, reduced litter size and increased pup mortality. No such effect was observed by PubMed:18755794. One possible explanation lies in the fact that PubMed:16861741 observed a remarkable 100% survival of control pups and 16% mortality for mutant pups, while PubMed:18755794 observed 16% mortality for both wild-type and mutant pups. According to PubMed:18755794, the construct used in PubMed:16861741 may lead to expression of a truncated transcript that might have deleterious effects.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3