Reviewed, UniProtKB/Swiss-Prot A7MBU6 (T23OA_DANRE)

Names and origin

Protein names

Recommended name:
    Tryptophan 2,3-dioxygenase A
      Short name=TDO-A
    EC=1.13.11.11
Alternative name(s):
    Tryptophan pyrrolase A
      Short name=Tryptophanase A
    Tryptophan oxygenase A
      Short name=TRPO-A
      Short name=TO-A
    Tryptamin 2,3-dioxygenase A

Gene names

Name:	tdo2a
Synonyms:	tdo2

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tryptophan catabolic process to kynurenine Inferred from electronic annotation. Source: InterPro
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW tryptophan 2,3-dioxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Tryptophan 2,3-dioxygenase A		PRO_0000360075
Regions
	Region	41 – 45	5	Substrate binding By similarity
	Region	71 – 75	5	Substrate binding By similarity
Sites
	Metal binding	327	1	Iron (heme axial ligand) By similarity
	Binding site	143	1	Substrate By similarity
	Binding site	150	1	Heme By similarity
	Binding site	341	1	Substrate By similarity
Experimental info
	Sequence conflict	144	1	E → G in AAI50377. Ref.1
	Sequence conflict	337	1	S → R in AAI50377. Ref.1
	Sequence conflict	401	1	S → T in AAI51921. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	A7MBU6-1 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 49DEA8A593CCBF32	FASTA	406	48,045
	10 20 30 40 50 60 MSGCPYFQRK FLSTSKQHLK EEENDEAQTG INKASKGGLI YGDYLQLDKI VTSQVLQSEL 70 80 90 100 110 120 KGNKIHDEHL FIVTHQAYEL WFKQVLWELD SVREIFISGH VRDERNMLKV NTRIHRIVMI 130 140 150 160 170 180 FRLLLDQFAV LETMTALDFY DFREYLSPAS GFQSLQFRLL ENKIGVPHNQ RVPYNRRHYR 190 200 210 220 230 240 DNFRDQESEL LLHSEQEPTL LQLVEQWLER TPGLEEDGFN FWGKLEKNIF EGLRREKEHI 250 260 270 280 290 300 EQKPASERKE EMLAELIKQR DIFLSLFDEK RHDHLVSTGQ RRLSYKALQG ALMIYFYREE 310 320 330 340 350 360 PRFQVPFQLL TSLMDIDTLM TKWRYNHVCM VHRMIGSKDG TGGSSGYQYL RSTVSDRYKV 370 380 390 400 FVDLFNLATF LIPRDWVPKL DPSEHTFLYM AECCDSSYCS SSDDSD

References

[1]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Intestine.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC150376 mRNA. Translation: AAI50377.1. BC151920 mRNA. Translation: AAI51921.1.
IPI	IPI00866157.
RefSeq	NP_001096086.1.
UniGene	Dr.105118
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7MBU6.
Genome annotation databases
Ensembl	ENSDART00000105590; ENSDARP00000096367; ENSDARG00000071429; Danio rerio. [Genome view]
GeneID	100008541.
KEGG	dre:100008541.
Organism-specific databases
CTD	100008541.
ZFIN	ZDB-GENE-030131-1187. tdo2a.
Phylogenomic databases
HOGENOM	HBG647485.
OMA	HYRDNFR.
OrthoDB	EOG99KJ9W.
Gene expression databases
Bgee	A7MBU6.
Family and domain databases
InterPro	IPR004981. Trp_2_3_dOase. [Graphical view]
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

T23OA_DANRE

Accession

Primary (citable) accession number: A7MBU6
Secondary accession number(s): A7E2J5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	February 9, 2010
This is version 21 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Zebrafish annotation project