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Protein

Receptor-type tyrosine-protein phosphatase F

Gene

PTPRF

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Possible cell adhesion receptor. It possesses an intrinsic protein tyrosine phosphatase activity (PTPase) and dephosphorylates EPHA2 regulating its activity (By similarity).By similarity
The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1507SubstrateBy similarity1
Active sitei1539Phosphocysteine intermediateBy similarity1
Binding sitei1583SubstrateBy similarity1
Active sitei1830Phosphocysteine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase, Receptor

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiR-BTA-8849932. SALM protein interactions at the synapses.

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase F (EC:3.1.3.48)
Gene namesi
Name:PTPRF
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 3

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 1254ExtracellularSequence analysisAdd BLAST1225
Transmembranei1255 – 1275HelicalSequence analysisAdd BLAST21
Topological domaini1276 – 1898CytoplasmicSequence analysisAdd BLAST623

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000037019230 – 1898Receptor-type tyrosine-protein phosphatase FAdd BLAST1869

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 107PROSITE-ProRule annotation
Glycosylationi117N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi156 ↔ 207PROSITE-ProRule annotation
Glycosylationi250N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi253 ↔ 298PROSITE-ProRule annotation
Glycosylationi295N-linked (GlcNAc...)Sequence analysis1
Glycosylationi721N-linked (GlcNAc...)Sequence analysis1
Glycosylationi941N-linked (GlcNAc...)Sequence analysis1
Glycosylationi957N-linked (GlcNAc...)Sequence analysis1
Modified residuei1296PhosphoserineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiA7MBJ4.
PRIDEiA7MBJ4.

Expressioni

Gene expression databases

BgeeiENSBTAG00000000253.

Interactioni

Subunit structurei

Interacts with GRIP1. Interacts with PPFIA1, PPFIA2 and PPFIA3. Interacts with INSR.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024046.

Structurei

3D structure databases

ProteinModelPortaliA7MBJ4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 123Ig-like C2-type 1Add BLAST91
Domaini135 – 224Ig-like C2-type 2Add BLAST90
Domaini232 – 314Ig-like C2-type 3Add BLAST83
Domaini321 – 411Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST91
Domaini416 – 510Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST95
Domaini514 – 604Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST91
Domaini609 – 706Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST98
Domaini711 – 810Fibronectin type-III 5PROSITE-ProRule annotationAdd BLAST100
Domaini811 – 905Fibronectin type-III 6PROSITE-ProRule annotationAdd BLAST95
Domaini909 – 1001Fibronectin type-III 7PROSITE-ProRule annotationAdd BLAST93
Domaini1005 – 1089Fibronectin type-III 8PROSITE-ProRule annotationAdd BLAST85
Domaini1343 – 1598Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd BLAST256
Domaini1630 – 1889Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd BLAST260

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni68 – 77Heparin-bindingBy similarity10
Regioni1539 – 1545Substrate bindingBy similarity7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1164 – 1169Poly-Arg6

Sequence similaritiesi

Contains 8 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 2 tyrosine-protein phosphatase domains.PROSITE-ProRule annotation

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00760000118900.
HOGENOMiHOG000010250.
InParanoidiA7MBJ4.
KOiK05695.
OMAiMLTPRWS.
OrthoDBiEOG091G11WG.
TreeFamiTF312900.

Family and domain databases

CDDicd00063. FN3. 8 hits.
Gene3Di2.60.40.10. 11 hits.
3.90.190.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00041. fn3. 7 hits.
PF07679. I-set. 3 hits.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 8 hits.
SM00409. IG. 3 hits.
SM00408. IGc2. 3 hits.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 3 hits.
SSF49265. SSF49265. 5 hits.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 8 hits.
PS50835. IG_LIKE. 3 hits.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A7MBJ4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPEPAPGRT MVPLVPALVM LGLVAGAHGD SKPVFVKVPE DQTGLSGGVA
60 70 80 90 100
SFVCQATGEP KPRITWMKKG KKVSSQRFEV IEFDDGAGSV LRIQPLRVQR
110 120 130 140 150
DEAIYECTAT NSLGEINTSA KLSVLEEEQL PLGFPSIDMG PQLKVVEKAR
160 170 180 190 200
TATMLCAAGG NPDPEISWFK DFLPVDPAAS NGRIKQLRSG ALQIESSEES
210 220 230 240 250
DQGKYECVAT NSAGTRYSAP ANLYVRVRRV APRFSIPPSS QEVMPGGSVN
260 270 280 290 300
LTCVAVGAPM PYVKWMMGAE ELTKEDEMPV GRNVLELSNV VRSANYTCVA
310 320 330 340 350
ISSLGMIEAT AQVTVKALPK PPIDLVVTET TATSVTLTWD SGNSEPVSYY
360 370 380 390 400
GIQYRPAGAE GPFQEVDGVA TTRYSIGGLS PFSEYAFRVL AVNSIGRGPP
410 420 430 440 450
SEAVRARTGE QAPSSPPRRV QARMLSASTM LVQWEPPEEP NGLVRGYRVY
460 470 480 490 500
YTPDSRRPLS AWHKHNTDAG LLTTVGSLLP GITYSLRVLA FTAVGDGPPS
510 520 530 540 550
PTIQVKTQQG VPAQPADFQA EVDSDTRIQL SWLLPPQERI VKYELVYWAA
560 570 580 590 600
EDEGQQHKVT FDPTSSYTVE DLKPDTLYRF QLAARSELGV GVFTPTIEAR
610 620 630 640 650
TAQSTPSAPP QKVTCVSVGS TTVRVSWVPP PADSRNGVIT QYSVAYEAVD
660 670 680 690 700
GEDRGRHVVD GIGREHSSWD LVGLEKWTEY RVWVRAHTDV GPGPESSPVL
710 720 730 740 750
VRTDEDVPSG PPRKVEVEPL NSTAVRVSWK LPVPSKQHGQ IRGYQVTYVR
760 770 780 790 800
LENGEPRGAP IIQDVMLAEA QETTISGLTP ETTYSITVAA YTTKGDGARS
810 820 830 840 850
KPKIVTTTGA VPGRPTMMVS TTAMNTALLQ WHPPKELPGE LLGYRLQYRR
860 870 880 890 900
ADEARPSTID FGKDDQHFTV TGLHKGATYI FRLTAKNRAG LGEEFEKEIT
910 920 930 940 950
TPEDVPSGFP QNLRVIGLTT STTELIWDPP VLAERNGRIT NYTVVYRDIN
960 970 980 990 1000
SQQELQNVTA DTHLTLSGLK PDTTYDIKVR ARTSKGAGPL SPSIQSRTMP
1010 1020 1030 1040 1050
VEQVFAKNFR VEAAMKTSVL LSWEVPDSYK SAVPFRILYN GQSVEVDGHS
1060 1070 1080 1090 1100
MRKLIADLQP NTEYSFVLMN RGSSAGGLQH LVSIRTAPDL LPHKPLPASA
1110 1120 1130 1140 1150
YIEDGRFTLT MPRVQEPALV RWFYIMVVPI DRMGGSMLAP QWSTPEELEL
1160 1170 1180 1190 1200
DELLEAIEQG GGERLRRRRQ TERLKPYVAA QVDVLPETFT LGDKKNYQGF
1210 1220 1230 1240 1250
YNRPLSPDLS YQCFVLASLK EPVDQKRYAC SPYSDEIVVQ VTPAQQQEEP
1260 1270 1280 1290 1300
ELLWVTGPVL AVILIVLIVI AILLFKRKRT HSPSSKDEQS IGLKDSLLAH
1310 1320 1330 1340 1350
SSDPVEMRRL NYQTPGMRDH PPIPITDLAD NIERLKANDG LKFSQEYESI
1360 1370 1380 1390 1400
DPGQQFTWEN SNLEVNKPKN RYANVIAYDH SRVILTSIDG VPGSDYINAN
1410 1420 1430 1440 1450
YIDGYRKQNA YIATQGPLPE TMGDFWRMVW EQRTATVVMM TRLEEKSRVK
1460 1470 1480 1490 1500
CDQYWPARGT ETYGLIQVTL LDTVELATYT VRTFALYKSG SSEKRELRQF
1510 1520 1530 1540 1550
QFMAWPDHGV PEYPTPILAF LRRVKACNPL DAGPMVVHCS AGVGRTGCFI
1560 1570 1580 1590 1600
VIDAMLERMK HEKTVDIYGH VTCMRAQRNY MVQTEDQYVF IHEALLEAAM
1610 1620 1630 1640 1650
CGHTEVPARN LYAHIQKLGQ VPPGESVTAM ELEFKLLANS KAHTSRFISA
1660 1670 1680 1690 1700
NLPCNKFKNR LVNIMPYELT RVCLQPIRGV EGSDYINASF LDGYRQQKAY
1710 1720 1730 1740 1750
IATQGPLAES TEDFWRMLWE HNSTIIVMLT RLREMGREKC HQYWPAERSA
1760 1770 1780 1790 1800
RYQYFVVDPM AEYNMPQYIL REFKVTDARD GQSRTIRQFQ FTDWPEQGVP
1810 1820 1830 1840 1850
KTGEGFIDFI GQVHKTKEQF GQDGPITVHC SAGVGRTGVF ITLSIVLERM
1860 1870 1880 1890
RYEGVVDMFQ TVKTLRTQRP AMVQTEDQYQ LCYRAALEYL GSFDHYAT
Length:1,898
Mass (Da):211,382
Last modified:October 2, 2007 - v1
Checksum:i863570011A8BF6F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC151592 mRNA. Translation: AAI51593.1.
RefSeqiNP_001094549.1. NM_001101079.1.
UniGeneiBt.5379.

Genome annotation databases

EnsembliENSBTAT00000024046; ENSBTAP00000024046; ENSBTAG00000000253.
GeneIDi512072.
KEGGibta:512072.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC151592 mRNA. Translation: AAI51593.1.
RefSeqiNP_001094549.1. NM_001101079.1.
UniGeneiBt.5379.

3D structure databases

ProteinModelPortaliA7MBJ4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024046.

Proteomic databases

PaxDbiA7MBJ4.
PRIDEiA7MBJ4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000024046; ENSBTAP00000024046; ENSBTAG00000000253.
GeneIDi512072.
KEGGibta:512072.

Organism-specific databases

CTDi5792.

Phylogenomic databases

eggNOGiKOG4228. Eukaryota.
COG5599. LUCA.
GeneTreeiENSGT00760000118900.
HOGENOMiHOG000010250.
InParanoidiA7MBJ4.
KOiK05695.
OMAiMLTPRWS.
OrthoDBiEOG091G11WG.
TreeFamiTF312900.

Enzyme and pathway databases

ReactomeiR-BTA-8849932. SALM protein interactions at the synapses.

Gene expression databases

BgeeiENSBTAG00000000253.

Family and domain databases

CDDicd00063. FN3. 8 hits.
Gene3Di2.60.40.10. 11 hits.
3.90.190.10. 2 hits.
InterProiIPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
[Graphical view]
PfamiPF00041. fn3. 7 hits.
PF07679. I-set. 3 hits.
PF00102. Y_phosphatase. 2 hits.
[Graphical view]
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiSM00060. FN3. 8 hits.
SM00409. IG. 3 hits.
SM00408. IGc2. 3 hits.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 3 hits.
SSF49265. SSF49265. 5 hits.
SSF52799. SSF52799. 2 hits.
PROSITEiPS50853. FN3. 8 hits.
PS50835. IG_LIKE. 3 hits.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTPRF_BOVIN
AccessioniPrimary (citable) accession number: A7MBJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: October 2, 2007
Last modified: November 30, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.