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A7MBI7

- COMT_BOVIN

UniProt

A7MBI7 - COMT_BOVIN

Protein

Catechol O-methyltransferase

Gene

COMT

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol By similarity.By similarity

    Catalytic activityi

    S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei93 – 931S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
    Binding sitei115 – 1151S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei123 – 1231S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei141 – 1411S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei170 – 1701S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
    Metal bindingi192 – 1921MagnesiumBy similarity
    Binding sitei192 – 1921S-adenosyl-L-methioninePROSITE-ProRule annotation
    Binding sitei195 – 1951SubstrateBy similarity
    Metal bindingi220 – 2201MagnesiumBy similarity
    Metal bindingi221 – 2211MagnesiumBy similarity
    Binding sitei221 – 2211SubstrateBy similarity
    Binding sitei250 – 2501SubstrateBy similarity

    GO - Molecular functioni

    1. catechol O-methyltransferase activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. dopamine catabolic process Source: Ensembl
    2. neurotransmitter catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Catecholamine metabolism, Neurotransmitter degradation

    Keywords - Ligandi

    Magnesium, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    ReactomeiREACT_202958. Methylation.
    REACT_209234. Enzymatic degradation of dopamine by COMT.
    REACT_215107. Enzymatic degradation of Dopamine by monoamine oxidase.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Catechol O-methyltransferase (EC:2.1.1.6)
    Gene namesi
    Name:COMT
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Isoform 2 : Cytoplasm By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrion Source: Ensembl
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 272272Catechol O-methyltransferasePRO_0000318146Add
    BLAST

    Proteomic databases

    PRIDEiA7MBI7.

    Interactioni

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000025997.

    Structurei

    3D structure databases

    ProteinModelPortaliA7MBI7.
    SMRiA7MBI7. Positions 53-265.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni168 – 1714S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG4122.
    HOGENOMiHOG000046392.
    HOVERGENiHBG005376.
    InParanoidiA7MBI7.
    KOiK00545.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR025782. Catechol_O-MeTrfase.
    IPR017128. Catechol_O-MeTrfase_euk.
    IPR002935. O-MeTrfase_3.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PANTHERiPTHR10509. PTHR10509. 1 hit.
    PfamiPF01596. Methyltransf_3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS51682. SAM_OMT_I. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative initiation. Align

    Isoform 1 (identifier: A7MBI7-1) [UniParc]FASTAAdd to Basket

    Also known as: Membrane-bound, MB-COMT

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MLEAPPLLLV AGGVGLALLA LRWLATTDLQ FFGRAFIVWN EFIMKPIRNL    50
    LMGSSKEQRI LQHVLQHAVA GDPQSVVAAI DSYSLEKEWA MHVGEKKGQI 100
    VDRVLREQQP SVLLELGAYC GYSAVRMARL LLPGARLLTI EFNPDYAAIT 150
    QRMVEFAGLQ DKVTVVLGAS QDIIPQLKKK YDVDTLDMVF LDHWKDRYLP 200
    DMLLLEECGL LREGTVLLAD NVIYPGAPDF LEYVRGNSRF ECSHFSSYLE 250
    YSKVVDGLEK VVYKGLSGPA RP 272
    Length:272
    Mass (Da):30,485
    Last modified:October 2, 2007 - v1
    Checksum:iEBBA0B76A5D4C4ED
    GO
    Isoform 2 (identifier: A7MBI7-2) [UniParc]FASTAAdd to Basket

    Also known as: Soluble, S-COMT

    The sequence of this isoform differs from the canonical sequence as follows:
         1-51: Missing.

    Show »
    Length:221
    Mass (Da):24,792
    Checksum:i6B51B6A3F2805880
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 5151Missing in isoform 2. CuratedVSP_031175Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC151585 mRNA. Translation: AAI51586.1.
    RefSeqiNP_001095787.1. NM_001102317.1. [A7MBI7-1]
    UniGeneiBt.33191.

    Genome annotation databases

    GeneIDi618278.
    KEGGibta:618278.

    Keywords - Coding sequence diversityi

    Alternative initiation

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC151585 mRNA. Translation: AAI51586.1 .
    RefSeqi NP_001095787.1. NM_001102317.1. [A7MBI7-1 ]
    UniGenei Bt.33191.

    3D structure databases

    ProteinModelPortali A7MBI7.
    SMRi A7MBI7. Positions 53-265.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000025997.

    Proteomic databases

    PRIDEi A7MBI7.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 618278.
    KEGGi bta:618278.

    Organism-specific databases

    CTDi 1312.

    Phylogenomic databases

    eggNOGi COG4122.
    HOGENOMi HOG000046392.
    HOVERGENi HBG005376.
    InParanoidi A7MBI7.
    KOi K00545.

    Enzyme and pathway databases

    Reactomei REACT_202958. Methylation.
    REACT_209234. Enzymatic degradation of dopamine by COMT.
    REACT_215107. Enzymatic degradation of Dopamine by monoamine oxidase.

    Miscellaneous databases

    NextBioi 20901099.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR025782. Catechol_O-MeTrfase.
    IPR017128. Catechol_O-MeTrfase_euk.
    IPR002935. O-MeTrfase_3.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    PANTHERi PTHR10509. PTHR10509. 1 hit.
    Pfami PF01596. Methyltransf_3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS51682. SAM_OMT_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Thymus.

    Entry informationi

    Entry nameiCOMT_BOVIN
    AccessioniPrimary (citable) accession number: A7MBI7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 5, 2008
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3