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A7MBI7 (COMT_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Catechol O-methyltransferase

EC=2.1.1.6
Gene names
Name:COMT
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length272 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol By similarity.

Catalytic activity

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Subcellular location

Isoform 2: Cytoplasm By similarity.

Isoform 1: Cell membrane; Single-pass type II membrane protein; Extracellular side By similarity.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform 1 (identifier: A7MBI7-1)

Also known as: Membrane-bound; MB-COMT;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: A7MBI7-2)

Also known as: Soluble; S-COMT;

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 272272Catechol O-methyltransferase
PRO_0000318146

Regions

Transmembrane7 – 2721Helical; Signal-anchor for type II membrane protein; Potential
Region168 – 1714S-adenosyl-L-methionine binding By similarity

Sites

Metal binding1921Magnesium By similarity
Metal binding2201Magnesium By similarity
Metal binding2211Magnesium By similarity
Binding site931S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site1151S-adenosyl-L-methionine By similarity
Binding site1231S-adenosyl-L-methionine By similarity
Binding site1411S-adenosyl-L-methionine By similarity
Binding site1701S-adenosyl-L-methionine; via amide nitrogen By similarity
Binding site1921S-adenosyl-L-methionine By similarity
Binding site1951Substrate By similarity
Binding site2211Substrate By similarity
Binding site2501Substrate By similarity

Natural variations

Alternative sequence1 – 5151Missing in isoform 2.
VSP_031175

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Membrane-bound) (MB-COMT) [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: EBBA0B76A5D4C4ED

FASTA27230,485
        10         20         30         40         50         60 
MLEAPPLLLV AGGVGLALLA LRWLATTDLQ FFGRAFIVWN EFIMKPIRNL LMGSSKEQRI 

        70         80         90        100        110        120 
LQHVLQHAVA GDPQSVVAAI DSYSLEKEWA MHVGEKKGQI VDRVLREQQP SVLLELGAYC 

       130        140        150        160        170        180 
GYSAVRMARL LLPGARLLTI EFNPDYAAIT QRMVEFAGLQ DKVTVVLGAS QDIIPQLKKK 

       190        200        210        220        230        240 
YDVDTLDMVF LDHWKDRYLP DMLLLEECGL LREGTVLLAD NVIYPGAPDF LEYVRGNSRF 

       250        260        270 
ECSHFSSYLE YSKVVDGLEK VVYKGLSGPA RP 

« Hide

Isoform 2 (Soluble) (S-COMT) [UniParc].

Checksum: 6B51B6A3F2805880
Show »

FASTA22124,792

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Thymus.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC151585 mRNA. Translation: AAI51586.1.
RefSeqNP_001095787.1. NM_001102317.1. [A7MBI7-1]
UniGeneBt.33191.

3D structure databases

ProteinModelPortalA7MBI7.
SMRA7MBI7. Positions 53-265.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000025997.

Proteomic databases

PRIDEA7MBI7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID618278.
KEGGbta:618278.

Organism-specific databases

CTD1312.

Phylogenomic databases

eggNOGCOG4122.
HOGENOMHOG000046392.
HOVERGENHBG005376.
InParanoidA7MBI7.
KOK00545.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR025782. Catechol_O-MeTrfase.
IPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10509. PTHR10509. 1 hit.
PfamPF01596. Methyltransf_3. 1 hit.
[Graphical view]
PIRSFPIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
SUPFAMSSF53335. SSF53335. 1 hit.
PROSITEPS51682. SAM_OMT_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20901099.

Entry information

Entry nameCOMT_BOVIN
AccessionPrimary (citable) accession number: A7MBI7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 2, 2007
Last modified: June 11, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families