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A7MBI7

- COMT_BOVIN

UniProt

A7MBI7 - COMT_BOVIN

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Protein

Catechol O-methyltransferase

Gene

COMT

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol.

Cofactori

Mg2+By similarityNote: Binds 1 Mg(2+) ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Binding sitei115 – 1151S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei123 – 1231S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei141 – 1411S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei170 – 1701S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation
Metal bindingi192 – 1921MagnesiumBy similarity
Binding sitei192 – 1921S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei195 – 1951SubstrateBy similarity
Metal bindingi220 – 2201MagnesiumBy similarity
Metal bindingi221 – 2211MagnesiumBy similarity
Binding sitei221 – 2211SubstrateBy similarity
Binding sitei250 – 2501SubstrateBy similarity

GO - Molecular functioni

  1. catechol O-methyltransferase activity Source: UniProtKB-EC
  2. magnesium ion binding Source: InterPro

GO - Biological processi

  1. cellular response to phosphate starvation Source: Ensembl
  2. dopamine catabolic process Source: Ensembl
  3. neurotransmitter catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Catecholamine metabolism, Neurotransmitter degradation

Keywords - Ligandi

Magnesium, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiREACT_202958. Methylation.
REACT_209234. Enzymatic degradation of dopamine by COMT.
REACT_215107. Enzymatic degradation of Dopamine by monoamine oxidase.

Names & Taxonomyi

Protein namesi
Recommended name:
Catechol O-methyltransferase (EC:2.1.1.6)
Gene namesi
Name:COMT
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Isoform 2 : Cytoplasm By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
  2. integral component of membrane Source: UniProtKB-KW
  3. mitochondrion Source: Ensembl
  4. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 272272Catechol O-methyltransferasePRO_0000318146Add
BLAST

Proteomic databases

PRIDEiA7MBI7.

Expressioni

Gene expression databases

ExpressionAtlasiA7MBI7. baseline.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000025997.

Structurei

3D structure databases

ProteinModelPortaliA7MBI7.
SMRiA7MBI7. Positions 53-265.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni168 – 1714S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Cation-dependent O-methyltransferase family.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG4122.
HOGENOMiHOG000046392.
HOVERGENiHBG005376.
InParanoidiA7MBI7.
KOiK00545.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025782. Catechol_O-MeTrfase.
IPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERiPTHR10509. PTHR10509. 1 hit.
PfamiPF01596. Methyltransf_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51682. SAM_OMT_I. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. Align

Isoform 1 (identifier: A7MBI7-1) [UniParc]FASTAAdd to Basket

Also known as: Membrane-bound, MB-COMT

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLEAPPLLLV AGGVGLALLA LRWLATTDLQ FFGRAFIVWN EFIMKPIRNL
60 70 80 90 100
LMGSSKEQRI LQHVLQHAVA GDPQSVVAAI DSYSLEKEWA MHVGEKKGQI
110 120 130 140 150
VDRVLREQQP SVLLELGAYC GYSAVRMARL LLPGARLLTI EFNPDYAAIT
160 170 180 190 200
QRMVEFAGLQ DKVTVVLGAS QDIIPQLKKK YDVDTLDMVF LDHWKDRYLP
210 220 230 240 250
DMLLLEECGL LREGTVLLAD NVIYPGAPDF LEYVRGNSRF ECSHFSSYLE
260 270
YSKVVDGLEK VVYKGLSGPA RP
Length:272
Mass (Da):30,485
Last modified:October 2, 2007 - v1
Checksum:iEBBA0B76A5D4C4ED
GO
Isoform 2 (identifier: A7MBI7-2) [UniParc]FASTAAdd to Basket

Also known as: Soluble, S-COMT

The sequence of this isoform differs from the canonical sequence as follows:
     1-51: Missing.

Show »
Length:221
Mass (Da):24,792
Checksum:i6B51B6A3F2805880
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 5151Missing in isoform 2. CuratedVSP_031175Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC151585 mRNA. Translation: AAI51586.1.
RefSeqiNP_001095787.1. NM_001102317.1. [A7MBI7-1]
UniGeneiBt.33191.

Genome annotation databases

GeneIDi618278.
KEGGibta:618278.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC151585 mRNA. Translation: AAI51586.1 .
RefSeqi NP_001095787.1. NM_001102317.1. [A7MBI7-1 ]
UniGenei Bt.33191.

3D structure databases

ProteinModelPortali A7MBI7.
SMRi A7MBI7. Positions 53-265.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000025997.

Proteomic databases

PRIDEi A7MBI7.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 618278.
KEGGi bta:618278.

Organism-specific databases

CTDi 1312.

Phylogenomic databases

eggNOGi COG4122.
HOGENOMi HOG000046392.
HOVERGENi HBG005376.
InParanoidi A7MBI7.
KOi K00545.

Enzyme and pathway databases

Reactomei REACT_202958. Methylation.
REACT_209234. Enzymatic degradation of dopamine by COMT.
REACT_215107. Enzymatic degradation of Dopamine by monoamine oxidase.

Miscellaneous databases

NextBioi 20901099.

Gene expression databases

ExpressionAtlasi A7MBI7. baseline.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR025782. Catechol_O-MeTrfase.
IPR017128. Catechol_O-MeTrfase_euk.
IPR002935. O-MeTrfase_3.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
PANTHERi PTHR10509. PTHR10509. 1 hit.
Pfami PF01596. Methyltransf_3. 1 hit.
[Graphical view ]
PIRSFi PIRSF037177. Catechol_O-mtfrase_euk. 1 hit.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51682. SAM_OMT_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Thymus.

Entry informationi

Entry nameiCOMT_BOVIN
AccessioniPrimary (citable) accession number: A7MBI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: October 2, 2007
Last modified: November 26, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3