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A7MBI0

- PACN1_BOVIN

UniProt

A7MBI0 - PACN1_BOVIN

Protein

Protein kinase C and casein kinase substrate in neurons protein 1

Gene

PACSIN1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Binds to membranes via its F-BAR domain and mediates membrane tubulation. Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission By similarity.By similarity

    GO - Molecular functioni

    1. phospholipid binding Source: UniProtKB

    GO - Biological processi

    1. actin filament organization Source: InterPro
    2. membrane tubulation Source: UniProtKB
    3. negative regulation of endocytosis Source: Ensembl
    4. neuron projection morphogenesis Source: UniProtKB
    5. protein localization to membrane Source: UniProtKB
    6. synaptic vesicle endocytosis Source: UniProtKB

    Keywords - Biological processi

    Endocytosis

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein kinase C and casein kinase substrate in neurons protein 1
    Gene namesi
    Name:PACSIN1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 23

    Subcellular locationi

    Cytoplasm By similarity. Cell projection By similarity. Cell junctionsynapsesynaptosome By similarity. Cell projectionruffle membrane By similarity. Membrane By similarity; Peripheral membrane protein By similarity. Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity. Cell junctionsynapse By similarity. Cytoplasmcytosol By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: Colocalizes with MAPT in axons. In primary neuronal cultures, present at a high level in presynaptic nerve terminals and in the cell body. Colocalizes with DNM1 at vesicular structures in the cell body and neurites. Associates with membranes via its F-BAR domain By similarity.By similarity

    GO - Cellular componenti

    1. axon terminus Source: UniProtKB
    2. cell junction Source: UniProtKB-KW
    3. COPI-coated vesicle Source: Ensembl
    4. cytoplasm Source: UniProtKB
    5. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    6. cytosol Source: UniProtKB-SubCell
    7. ruffle membrane Source: UniProtKB
    8. synapse Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 444444Protein kinase C and casein kinase substrate in neurons protein 1PRO_0000351649Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei79 – 791PhosphoserineBy similarity
    Modified residuei184 – 1841PhosphothreonineBy similarity
    Modified residuei394 – 3941PhosphotyrosineBy similarity

    Post-translational modificationi

    Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).Curated

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Homodimer. May form heterooligomers with other PACSINs. Interacts with MAPT By similarity. Interacts (via SH3 domain) with SYNJ1 and WASL. Interacts (via SH3 domain) with DNM1; the interaction is reduced by DNM1 phosphorylation. Interacts with DNM2 and DNM3. Interacts with both COBL and DBNL. Identified in a complex composed of COBL, PACSIN1 and WASL. Interacts with EHD1 and EHD3. Interacts with TRPV4 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000021459.

    Structurei

    3D structure databases

    ProteinModelPortaliA7MBI0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini13 – 7664FCHPROSITE-ProRule annotationAdd
    BLAST
    Domaini385 – 44460SH3PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 307307F-BAR domainBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili26 – 275250By similarityAdd
    BLAST

    Domaini

    The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain. DNM1 binding abolishes autoinhibition By similarity.By similarity

    Sequence similaritiesi

    Belongs to the PACSIN family.Curated
    Contains 1 FCH domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, SH3 domain

    Phylogenomic databases

    eggNOGiNOG283356.
    GeneTreeiENSGT00510000046376.
    HOGENOMiHOG000007245.
    HOVERGENiHBG053486.
    InParanoidiA7MBI0.
    OMAiAMTREAN.
    OrthoDBiEOG75TMBJ.
    TreeFamiTF313677.

    Family and domain databases

    InterProiIPR001060. FCH_dom.
    IPR028518. PACSIN1.
    IPR001452. SH3_domain.
    [Graphical view]
    PANTHERiPTHR10959:SF7. PTHR10959:SF7. 1 hit.
    PfamiPF00611. FCH. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view]
    PRINTSiPR00452. SH3DOMAIN.
    SMARTiSM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7MBI0-1 [UniParc]FASTAAdd to Basket

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    MSGSYDEASL APEETTDSFW EVGNYKRTVK RIDDGHRLCN DLMSCVQERA    50
    KIEKAYAQQL TDWAKRWRQL LEKGPQYGSL ERAWGAIMTE ADKVSELHQE 100
    MKNSLLNEDL EKVKNWQKDA YHKQIMGGFK ETKEAEDGFR KAQKPWAKKM 150
    KELEAAKKAY HLACKEEKLA VTREMNSKTE QSVTPEQQKK LQDKVDKCKQ 200
    DVQKTQEKYE KVLDDVGKTT PQYMEGMEQV FEQCQQFEEK RLVFLKEVLL 250
    DIKRHLNLAE SSSYVQVYRE LEQAIRGADA QDDLRWFRST SGPGMPMNWP 300
    QFEEWNPDLP HTAAKKEKQP KKAEGAALTN AAGVVESTSQ AGDRGSVSSY 350
    DRGQTYATEW SDDESGNPFG GSEANGGSNP FDEDAKGVRV RALYDYDGQE 400
    QDELSFKAGD ELTKLGEEDE QGWCRGRLDS GQLGLYPANY VEVV 444
    Length:444
    Mass (Da):50,658
    Last modified:October 2, 2007 - v1
    Checksum:i96B816669F12EE76
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti108 – 1081E → Q in AAI53242. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC151572 mRNA. Translation: AAI51573.1.
    BC153241 mRNA. Translation: AAI53242.1.
    RefSeqiNP_001094571.1. NM_001101101.1.
    UniGeneiBt.35936.

    Genome annotation databases

    EnsembliENSBTAT00000021459; ENSBTAP00000021459; ENSBTAG00000016124.
    GeneIDi520488.
    KEGGibta:520488.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC151572 mRNA. Translation: AAI51573.1 .
    BC153241 mRNA. Translation: AAI53242.1 .
    RefSeqi NP_001094571.1. NM_001101101.1.
    UniGenei Bt.35936.

    3D structure databases

    ProteinModelPortali A7MBI0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000021459.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000021459 ; ENSBTAP00000021459 ; ENSBTAG00000016124 .
    GeneIDi 520488.
    KEGGi bta:520488.

    Organism-specific databases

    CTDi 29993.

    Phylogenomic databases

    eggNOGi NOG283356.
    GeneTreei ENSGT00510000046376.
    HOGENOMi HOG000007245.
    HOVERGENi HBG053486.
    InParanoidi A7MBI0.
    OMAi AMTREAN.
    OrthoDBi EOG75TMBJ.
    TreeFami TF313677.

    Miscellaneous databases

    NextBioi 20873115.

    Family and domain databases

    InterProi IPR001060. FCH_dom.
    IPR028518. PACSIN1.
    IPR001452. SH3_domain.
    [Graphical view ]
    PANTHERi PTHR10959:SF7. PTHR10959:SF7. 1 hit.
    Pfami PF00611. FCH. 1 hit.
    PF14604. SH3_9. 1 hit.
    [Graphical view ]
    PRINTSi PR00452. SH3DOMAIN.
    SMARTi SM00055. FCH. 1 hit.
    SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50133. FCH. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal brain and Fetal skin.

    Entry informationi

    Entry nameiPACN1_BOVIN
    AccessioniPrimary (citable) accession number: A7MBI0
    Secondary accession number(s): A7Z043
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 14, 2008
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3