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Protein

Protein kinase C and casein kinase substrate in neurons protein 1

Gene

PACSIN1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Binds to membranes via its F-BAR domain and mediates membrane tubulation. Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosis
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-BTA-8856828 Clathrin-mediated endocytosis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C and casein kinase substrate in neurons protein 1
Gene namesi
Name:PACSIN1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 23

Organism-specific databases

VGNCiVGNC:32542 PACSIN1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003516491 – 444Protein kinase C and casein kinase substrate in neurons protein 1Add BLAST444

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2PhosphoserineBy similarity1
Modified residuei79PhosphoserineBy similarity1
Modified residuei184PhosphothreonineBy similarity1
Modified residuei346PhosphoserineBy similarity1
Modified residuei348PhosphoserineBy similarity1
Modified residuei349PhosphoserineBy similarity1
Modified residuei361PhosphoserineBy similarity1
Modified residuei365PhosphoserineBy similarity1
Modified residuei394PhosphotyrosineBy similarity1
Modified residuei405PhosphoserineBy similarity1
Modified residuei430PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).Curated

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiA7MBI0
PRIDEiA7MBI0

Expressioni

Gene expression databases

BgeeiENSBTAG00000016124

Interactioni

Subunit structurei

Homodimer. May form heterooligomers with other PACSINs. Interacts with MAPT (By similarity). Interacts (via SH3 domain) with SYNJ1 and WASL. Interacts (via SH3 domain) with DNM1; the interaction is reduced by DNM1 phosphorylation. Interacts with DNM2 and DNM3. Interacts with both COBL and DBNL. Identified in a complex composed of COBL, PACSIN1 and WASL. Interacts with EHD1 and EHD3. Interacts with TRPV4 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021459

Structurei

3D structure databases

ProteinModelPortaliA7MBI0
SMRiA7MBI0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 283F-BARPROSITE-ProRule annotationAdd BLAST271
Domaini385 – 444SH3PROSITE-ProRule annotationAdd BLAST60

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili26 – 275By similarityAdd BLAST250

Domaini

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain. DNM1 binding abolishes autoinhibition (By similarity).By similarity

Sequence similaritiesi

Belongs to the PACSIN family.Curated

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiKOG2856 Eukaryota
ENOG410XRX2 LUCA
GeneTreeiENSGT00510000046376
HOGENOMiHOG000007245
HOVERGENiHBG053486
InParanoidiA7MBI0
KOiK20123
OMAiHQEVKNS
OrthoDBiEOG091G0AS9
TreeFamiTF313677

Family and domain databases

CDDicd07680 F-BAR_PACSIN1, 1 hit
cd11998 SH3_PACSIN1-2, 1 hit
Gene3Di1.20.1270.60, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR031160 F_BAR
IPR001060 FCH_dom
IPR028518 PACSIN1
IPR035743 PACSIN1/PACSIN2_SH3
IPR037454 PACSIN1_F-BAR
IPR036028 SH3-like_dom_sf
IPR001452 SH3_domain
PANTHERiPTHR23065:SF16 PTHR23065:SF16, 1 hit
PfamiView protein in Pfam
PF00611 FCH, 1 hit
PF14604 SH3_9, 1 hit
PRINTSiPR00452 SH3DOMAIN
SMARTiView protein in SMART
SM00055 FCH, 1 hit
SM00326 SH3, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF50044 SSF50044, 1 hit
PROSITEiView protein in PROSITE
PS51741 F_BAR, 1 hit
PS50002 SH3, 1 hit

Sequencei

Sequence statusi: Complete.

A7MBI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSYDEASL APEETTDSFW EVGNYKRTVK RIDDGHRLCN DLMSCVQERA
60 70 80 90 100
KIEKAYAQQL TDWAKRWRQL LEKGPQYGSL ERAWGAIMTE ADKVSELHQE
110 120 130 140 150
MKNSLLNEDL EKVKNWQKDA YHKQIMGGFK ETKEAEDGFR KAQKPWAKKM
160 170 180 190 200
KELEAAKKAY HLACKEEKLA VTREMNSKTE QSVTPEQQKK LQDKVDKCKQ
210 220 230 240 250
DVQKTQEKYE KVLDDVGKTT PQYMEGMEQV FEQCQQFEEK RLVFLKEVLL
260 270 280 290 300
DIKRHLNLAE SSSYVQVYRE LEQAIRGADA QDDLRWFRST SGPGMPMNWP
310 320 330 340 350
QFEEWNPDLP HTAAKKEKQP KKAEGAALTN AAGVVESTSQ AGDRGSVSSY
360 370 380 390 400
DRGQTYATEW SDDESGNPFG GSEANGGSNP FDEDAKGVRV RALYDYDGQE
410 420 430 440
QDELSFKAGD ELTKLGEEDE QGWCRGRLDS GQLGLYPANY VEVV
Length:444
Mass (Da):50,658
Last modified:October 2, 2007 - v1
Checksum:i96B816669F12EE76
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti108E → Q in AAI53242 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC151572 mRNA Translation: AAI51573.1
BC153241 mRNA Translation: AAI53242.1
RefSeqiNP_001094571.1, NM_001101101.1
UniGeneiBt.35936

Genome annotation databases

EnsembliENSBTAT00000021459; ENSBTAP00000021459; ENSBTAG00000016124
GeneIDi520488
KEGGibta:520488

Similar proteinsi

Entry informationi

Entry nameiPACN1_BOVIN
AccessioniPrimary (citable) accession number: A7MBI0
Secondary accession number(s): A7Z043
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: October 2, 2007
Last modified: March 28, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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