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A7MBI0 (PACN1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein kinase C and casein kinase substrate in neurons protein 1
Gene names
Name:PACSIN1
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length444 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Binds to membranes via its F-BAR domain and mediates membrane tubulation. Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission By similarity.

Subunit structure

Homodimer. May form heterooligomers with other PACSINs. Interacts with MAPT By similarity. Interacts (via SH3 domain) with SYNJ1 and WASL. Interacts (via SH3 domain) with DNM1; the interaction is reduced by DNM1 phosphorylation. Interacts with DNM2 and DNM3. Interacts with both COBL and DBNL. Identified in a complex composed of COBL, PACSIN1 and WASL. Interacts with EHD1 and EHD3. Interacts with TRPV4 By similarity.

Subcellular location

Cytoplasm By similarity. Cell projection By similarity. Cell junctionsynapsesynaptosome By similarity. Cell projectionruffle membrane By similarity. Membrane; Peripheral membrane protein By similarity. Cytoplasmic vesicle membrane; Peripheral membrane protein By similarity. Cell junctionsynapse By similarity. Cytoplasmcytosol By similarity. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Colocalizes with MAPT in axons. In primary neuronal cultures, present at a high level in presynaptic nerve terminals and in the cell body. Colocalizes with DNM1 at vesicular structures in the cell body and neurites. Associates with membranes via its F-BAR domain By similarity.

Domain

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain. DNM1 binding abolishes autoinhibition By similarity.

Post-translational modification

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC) Probable.

Sequence similarities

Belongs to the PACSIN family.

Contains 1 FCH domain.

Contains 1 SH3 domain.

Ontologies

Keywords
   Biological processEndocytosis
   Cellular componentCell junction
Cell membrane
Cell projection
Cytoplasm
Cytoplasmic vesicle
Membrane
Synapse
Synaptosome
   DomainCoiled coil
SH3 domain
   LigandLipid-binding
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmembrane tubulation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of endocytosis

Inferred from electronic annotation. Source: Ensembl

neuron projection morphogenesis

Inferred from sequence or structural similarity. Source: UniProtKB

protein localization to membrane

Inferred from sequence or structural similarity. Source: UniProtKB

synaptic vesicle endocytosis

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentCOPI-coated vesicle

Inferred from electronic annotation. Source: Ensembl

axon terminus

Inferred from sequence or structural similarity. Source: UniProtKB

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytosol

Inferred from electronic annotation. Source: UniProtKB-SubCell

ruffle membrane

Inferred from sequence or structural similarity. Source: UniProtKB

synapse

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionphospholipid binding

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 444444Protein kinase C and casein kinase substrate in neurons protein 1
PRO_0000351649

Regions

Domain13 – 7664FCH
Domain385 – 44460SH3
Region1 – 307307F-BAR domain By similarity
Coiled coil26 – 275250 By similarity

Amino acid modifications

Modified residue791Phosphoserine By similarity
Modified residue1841Phosphothreonine By similarity
Modified residue3941Phosphotyrosine By similarity

Experimental info

Sequence conflict1081E → Q in AAI53242. Ref.1

Sequences

Sequence LengthMass (Da)Tools
A7MBI0 [UniParc].

Last modified October 2, 2007. Version 1.
Checksum: 96B816669F12EE76

FASTA44450,658
        10         20         30         40         50         60 
MSGSYDEASL APEETTDSFW EVGNYKRTVK RIDDGHRLCN DLMSCVQERA KIEKAYAQQL 

        70         80         90        100        110        120 
TDWAKRWRQL LEKGPQYGSL ERAWGAIMTE ADKVSELHQE MKNSLLNEDL EKVKNWQKDA 

       130        140        150        160        170        180 
YHKQIMGGFK ETKEAEDGFR KAQKPWAKKM KELEAAKKAY HLACKEEKLA VTREMNSKTE 

       190        200        210        220        230        240 
QSVTPEQQKK LQDKVDKCKQ DVQKTQEKYE KVLDDVGKTT PQYMEGMEQV FEQCQQFEEK 

       250        260        270        280        290        300 
RLVFLKEVLL DIKRHLNLAE SSSYVQVYRE LEQAIRGADA QDDLRWFRST SGPGMPMNWP 

       310        320        330        340        350        360 
QFEEWNPDLP HTAAKKEKQP KKAEGAALTN AAGVVESTSQ AGDRGSVSSY DRGQTYATEW 

       370        380        390        400        410        420 
SDDESGNPFG GSEANGGSNP FDEDAKGVRV RALYDYDGQE QDELSFKAGD ELTKLGEEDE 

       430        440 
QGWCRGRLDS GQLGLYPANY VEVV 

« Hide

References

[1]NIH - Mammalian Gene Collection (MGC) project
Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Fetal brain and Fetal skin.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC151572 mRNA. Translation: AAI51573.1.
BC153241 mRNA. Translation: AAI53242.1.
RefSeqNP_001094571.1. NM_001101101.1.
UniGeneBt.35936.

3D structure databases

ProteinModelPortalA7MBI0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000021459.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000021459; ENSBTAP00000021459; ENSBTAG00000016124.
GeneID520488.
KEGGbta:520488.

Organism-specific databases

CTD29993.

Phylogenomic databases

eggNOGNOG283356.
GeneTreeENSGT00510000046376.
HOGENOMHOG000007245.
HOVERGENHBG053486.
InParanoidA7MBI0.
OMAAMTREAN.
OrthoDBEOG75TMBJ.
TreeFamTF313677.

Family and domain databases

InterProIPR001060. FCH_dom.
IPR028518. PACSIN1.
IPR001452. SH3_domain.
[Graphical view]
PANTHERPTHR10959:SF7. PTHR10959:SF7. 1 hit.
PfamPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSPR00452. SH3DOMAIN.
SMARTSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS50133. FCH. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20873115.

Entry information

Entry namePACN1_BOVIN
AccessionPrimary (citable) accession number: A7MBI0
Secondary accession number(s): A7Z043
Entry history
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: October 2, 2007
Last modified: June 11, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families