A7MBI0 (PACN1_BOVIN) Reviewed, UniProtKB/Swiss-Prot
Last modified June 11, 2014. Version 44. History...
Names and origin
|Protein names||Recommended name:|
Protein kinase C and casein kinase substrate in neurons protein 1
|Organism||Bos taurus (Bovine) [Reference proteome]|
|Taxonomic identifier||9913 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos|
|Sequence length||444 AA.|
|Protein existence||Evidence at transcript level|
General annotation (Comments)
Binds to membranes via its F-BAR domain and mediates membrane tubulation. Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission By similarity.
Homodimer. May form heterooligomers with other PACSINs. Interacts with MAPT By similarity. Interacts (via SH3 domain) with SYNJ1 and WASL. Interacts (via SH3 domain) with DNM1; the interaction is reduced by DNM1 phosphorylation. Interacts with DNM2 and DNM3. Interacts with both COBL and DBNL. Identified in a complex composed of COBL, PACSIN1 and WASL. Interacts with EHD1 and EHD3. Interacts with TRPV4 By similarity.
Cytoplasm By similarity. Cell projection By similarity. Cell junction › synapse › synaptosome By similarity. Cell projection › ruffle membrane By similarity. Membrane; Peripheral membrane protein By similarity. Cytoplasmic vesicle membrane; Peripheral membrane protein By similarity. Cell junction › synapse By similarity. Cytoplasm › cytosol By similarity. Cell membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Colocalizes with MAPT in axons. In primary neuronal cultures, present at a high level in presynaptic nerve terminals and in the cell body. Colocalizes with DNM1 at vesicular structures in the cell body and neurites. Associates with membranes via its F-BAR domain By similarity.
The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain. DNM1 binding abolishes autoinhibition By similarity.
Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC) Probable.
Belongs to the PACSIN family.
Contains 1 FCH domain.
Contains 1 SH3 domain.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Chain||1 – 444||444||Protein kinase C and casein kinase substrate in neurons protein 1||PRO_0000351649|
|Domain||13 – 76||64||FCH|
|Domain||385 – 444||60||SH3|
|Region||1 – 307||307||F-BAR domain By similarity|
|Coiled coil||26 – 275||250||By similarity|
Amino acid modifications
|Modified residue||79||1||Phosphoserine By similarity|
|Modified residue||184||1||Phosphothreonine By similarity|
|Modified residue||394||1||Phosphotyrosine By similarity|
|Sequence conflict||108||1||E → Q in AAI53242. Ref.1|
|BC151572 mRNA. Translation: AAI51573.1.|
BC153241 mRNA. Translation: AAI53242.1.
|RefSeq||NP_001094571.1. NM_001101101.1. |
3D structure databases
Protein-protein interaction databases
Protocols and materials databases
Genome annotation databases
|Ensembl||ENSBTAT00000021459; ENSBTAP00000021459; ENSBTAG00000016124. |
Family and domain databases
|InterPro||IPR001060. FCH_dom. |
|PANTHER||PTHR10959:SF7. PTHR10959:SF7. 1 hit. |
|Pfam||PF00611. FCH. 1 hit. |
PF14604. SH3_9. 1 hit.
|PRINTS||PR00452. SH3DOMAIN. |
|SMART||SM00055. FCH. 1 hit. |
SM00326. SH3. 1 hit.
|SUPFAM||SSF50044. SSF50044. 1 hit. |
|PROSITE||PS50133. FCH. 1 hit. |
PS50002. SH3. 1 hit.
|Accession||Primary (citable) accession number: A7MBI0|
Secondary accession number(s): A7Z043
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|
Index of protein domains and families