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Protein

Protein kinase C and casein kinase substrate in neurons protein 1

Gene

PACSIN1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Binds to membranes via its F-BAR domain and mediates membrane tubulation. Plays a role in the reorganization of the microtubule cytoskeleton via its interaction with MAPT; this decreases microtubule stability and inhibits MAPT-induced microtubule polymerization. Plays a role in cellular transport processes by recruiting DNM1, DNM2 and DNM3 to membranes. Plays a role in the reorganization of the actin cytoskeleton and in neuron morphogenesis via its interaction with COBL and WASL, and by recruiting COBL to the cell cortex. Plays a role in the regulation of neurite formation, neurite branching and the regulation of neurite length. Required for normal synaptic vesicle endocytosis; this process retrieves previously released neurotransmitters to accommodate multiple cycles of neurotransmission. Required for normal excitatory and inhibitory synaptic transmission (By similarity).By similarity

GO - Molecular functioni

  1. cytoskeletal protein binding Source: GO_Central
  2. phospholipid binding Source: UniProtKB

GO - Biological processi

  1. actin filament organization Source: InterPro
  2. cytoskeleton organization Source: GO_Central
  3. membrane tubulation Source: UniProtKB
  4. negative regulation of endocytosis Source: Ensembl
  5. neuron projection morphogenesis Source: UniProtKB
  6. protein localization to membrane Source: UniProtKB
  7. regulation of endocytosis Source: GO_Central
  8. synaptic vesicle endocytosis Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Endocytosis

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein kinase C and casein kinase substrate in neurons protein 1
Gene namesi
Name:PACSIN1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 23

Subcellular locationi

Cytoplasm By similarity. Cell projection By similarity. Cell junctionsynapsesynaptosome By similarity. Cell projectionruffle membrane By similarity. Membrane By similarity; Peripheral membrane protein By similarity. Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity. Cell junctionsynapse By similarity. Cytoplasmcytosol By similarity. Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: Colocalizes with MAPT in axons. In primary neuronal cultures, present at a high level in presynaptic nerve terminals and in the cell body. Colocalizes with DNM1 at vesicular structures in the cell body and neurites. Associates with membranes via its F-BAR domain (By similarity).By similarity

GO - Cellular componenti

  1. axon terminus Source: UniProtKB
  2. cell junction Source: UniProtKB-KW
  3. COPI-coated vesicle Source: Ensembl
  4. cytoplasm Source: UniProtKB
  5. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  6. cytosol Source: UniProtKB-SubCell
  7. endosome Source: GO_Central
  8. membrane Source: GO_Central
  9. membrane-bounded vesicle Source: GO_Central
  10. neuron projection Source: GO_Central
  11. ruffle membrane Source: UniProtKB
  12. synapse Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Membrane, Synapse, Synaptosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 444444Protein kinase C and casein kinase substrate in neurons protein 1PRO_0000351649Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei79 – 791PhosphoserineBy similarity
Modified residuei184 – 1841PhosphothreonineBy similarity
Modified residuei394 – 3941PhosphotyrosineBy similarity

Post-translational modificationi

Phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC).Curated

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer. May form heterooligomers with other PACSINs. Interacts with MAPT (By similarity). Interacts (via SH3 domain) with SYNJ1 and WASL. Interacts (via SH3 domain) with DNM1; the interaction is reduced by DNM1 phosphorylation. Interacts with DNM2 and DNM3. Interacts with both COBL and DBNL. Identified in a complex composed of COBL, PACSIN1 and WASL. Interacts with EHD1 and EHD3. Interacts with TRPV4 (By similarity).By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021459.

Structurei

3D structure databases

ProteinModelPortaliA7MBI0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini13 – 283271F-BARPROSITE-ProRule annotationAdd
BLAST
Domaini385 – 44460SH3PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili26 – 275250By similarityAdd
BLAST

Domaini

The F-BAR domain forms a coiled coil and mediates membrane-binding and membrane tubulation. In the autoinhibited conformation, interaction with the SH3 domain inhibits membrane tubulation mediated by the F-BAR domain. DNM1 binding abolishes autoinhibition (By similarity).By similarity

Sequence similaritiesi

Belongs to the PACSIN family.Curated
Contains 1 F-BAR domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, SH3 domain

Phylogenomic databases

eggNOGiNOG283356.
GeneTreeiENSGT00510000046376.
HOGENOMiHOG000007245.
HOVERGENiHBG053486.
InParanoidiA7MBI0.
OMAiAMTREAN.
OrthoDBiEOG75TMBJ.
TreeFamiTF313677.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR028518. PACSIN1.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10959:SF7. PTHR10959:SF7. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MBI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSYDEASL APEETTDSFW EVGNYKRTVK RIDDGHRLCN DLMSCVQERA
60 70 80 90 100
KIEKAYAQQL TDWAKRWRQL LEKGPQYGSL ERAWGAIMTE ADKVSELHQE
110 120 130 140 150
MKNSLLNEDL EKVKNWQKDA YHKQIMGGFK ETKEAEDGFR KAQKPWAKKM
160 170 180 190 200
KELEAAKKAY HLACKEEKLA VTREMNSKTE QSVTPEQQKK LQDKVDKCKQ
210 220 230 240 250
DVQKTQEKYE KVLDDVGKTT PQYMEGMEQV FEQCQQFEEK RLVFLKEVLL
260 270 280 290 300
DIKRHLNLAE SSSYVQVYRE LEQAIRGADA QDDLRWFRST SGPGMPMNWP
310 320 330 340 350
QFEEWNPDLP HTAAKKEKQP KKAEGAALTN AAGVVESTSQ AGDRGSVSSY
360 370 380 390 400
DRGQTYATEW SDDESGNPFG GSEANGGSNP FDEDAKGVRV RALYDYDGQE
410 420 430 440
QDELSFKAGD ELTKLGEEDE QGWCRGRLDS GQLGLYPANY VEVV
Length:444
Mass (Da):50,658
Last modified:October 2, 2007 - v1
Checksum:i96B816669F12EE76
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti108 – 1081E → Q in AAI53242 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC151572 mRNA. Translation: AAI51573.1.
BC153241 mRNA. Translation: AAI53242.1.
RefSeqiNP_001094571.1. NM_001101101.1.
UniGeneiBt.35936.

Genome annotation databases

EnsembliENSBTAT00000021459; ENSBTAP00000021459; ENSBTAG00000016124.
GeneIDi520488.
KEGGibta:520488.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC151572 mRNA. Translation: AAI51573.1.
BC153241 mRNA. Translation: AAI53242.1.
RefSeqiNP_001094571.1. NM_001101101.1.
UniGeneiBt.35936.

3D structure databases

ProteinModelPortaliA7MBI0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000021459.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000021459; ENSBTAP00000021459; ENSBTAG00000016124.
GeneIDi520488.
KEGGibta:520488.

Organism-specific databases

CTDi29993.

Phylogenomic databases

eggNOGiNOG283356.
GeneTreeiENSGT00510000046376.
HOGENOMiHOG000007245.
HOVERGENiHBG053486.
InParanoidiA7MBI0.
OMAiAMTREAN.
OrthoDBiEOG75TMBJ.
TreeFamiTF313677.

Miscellaneous databases

NextBioi20873115.

Family and domain databases

InterProiIPR001060. FCH_dom.
IPR028518. PACSIN1.
IPR001452. SH3_domain.
[Graphical view]
PANTHERiPTHR10959:SF7. PTHR10959:SF7. 1 hit.
PfamiPF00611. FCH. 1 hit.
PF14604. SH3_9. 1 hit.
[Graphical view]
PRINTSiPR00452. SH3DOMAIN.
SMARTiSM00055. FCH. 1 hit.
SM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS51741. F_BAR. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal brain and Fetal skin.

Entry informationi

Entry nameiPACN1_BOVIN
AccessioniPrimary (citable) accession number: A7MBI0
Secondary accession number(s): A7Z043
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2008
Last sequence update: October 2, 2007
Last modified: March 4, 2015
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.