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Protein

Adenylosuccinate synthetase isozyme 2

Gene

ADSS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).By similarity

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: AMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase isozyme 2 (ADSS), Adenylosuccinate synthetase isozyme 1 (ADSSL1)
  2. Adenylosuccinate lyase (ADSL), Adenylosuccinate lyase (ADSL)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401Proton acceptorUniRule annotation
Metal bindingi40 – 401MagnesiumUniRule annotation
Binding sitei40 – 401SubstrateUniRule annotation
Metal bindingi67 – 671Magnesium; via carbonyl oxygenUniRule annotation
Active sitei68 – 681Proton donorUniRule annotation
Binding sitei162 – 1621IMPUniRule annotation
Binding sitei176 – 1761IMP; shared with dimeric partnerUniRule annotation
Binding sitei255 – 2551IMPUniRule annotation
Binding sitei270 – 2701IMPUniRule annotation
Binding sitei334 – 3341IMPUniRule annotation
Binding sitei336 – 3361GTPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 457GTPUniRule annotation
Nucleotide bindingi67 – 693GTPUniRule annotation
Nucleotide bindingi362 – 3643GTPUniRule annotation
Nucleotide bindingi444 – 4474GTPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00075; UER00335.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate synthetase isozyme 2UniRule annotation (EC:6.3.4.4UniRule annotation)
Short name:
AMPSase 2UniRule annotation
Short name:
AdSS 2UniRule annotation
Alternative name(s):
Adenylosuccinate synthetase, acidic isozymeUniRule annotation
Adenylosuccinate synthetase, liver isozymeUniRule annotation
Short name:
L-type adenylosuccinate synthetaseUniRule annotation
IMP--aspartate ligase 2UniRule annotation
Gene namesi
Name:ADSSUniRule annotation
Synonyms:ADSS2UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 16

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 456456Adenylosuccinate synthetase isozyme 2PRO_0000355565Add
BLAST

Proteomic databases

PaxDbiA7MBG0.
PRIDEiA7MBG0.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000044296.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni40 – 434IMP bindingUniRule annotation
Regioni65 – 684IMP bindingUniRule annotation
Regioni330 – 3367Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the adenylosuccinate synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1355. Eukaryota.
COG0104. LUCA.
GeneTreeiENSGT00390000015553.
HOGENOMiHOG000260959.
HOVERGENiHBG053768.
InParanoidiA7MBG0.
KOiK01939.
OMAiVQVKYEV.
OrthoDBiEOG7M6D76.
TreeFamiTF300486.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth.
MF_03127. Adenylosucc_synth_vert_acid.
InterProiIPR018220. Adenylosuccin_syn_GTP-bd.
IPR033128. Adenylosuccin_syn_Lys_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027529. AdSS_2_vert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MBG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFAETNPAT SSLPNGDCGR PRTRPGGNRV TVVLGAQWGD EGKGKVVDLL
60 70 80 90 100
AQDADIVCRC QGGNNAGHTV VVDSVEYDFH LLPSGIINPN VTAFIGNGVV
110 120 130 140 150
IHLPGLFEEA EKNVQKGKGL EGWEKRLIIS DRAHIVFDFH QAADGIQEQQ
160 170 180 190 200
RQEQAGKNLG TTKKGIGPVY SSKAARSGLR MCDLVSDFGG FSERFKVLAN
210 220 230 240 250
QYKSIYPTLE IDIEGELQKL KGYMERIKPM VRDGVYFLYE ALHGPPKKIL
260 270 280 290 300
VEGANAALLD IDFGTYPFVT SSNCTVGGVC TGLGMPPQNV GEVYGVVKAY
310 320 330 340 350
TTRVGIGAFP TEQDNEIGEL LQTRGREFGV TTGRKRRCGW LDLVLLKYAH
360 370 380 390 400
MINGFTALAL TKLDILDMFT EIKVGVAYKL DGEIIPHFPA NQEVLNKVEV
410 420 430 440 450
QYKTLPGWNT DISNARTFKE LPINAQNYVR FIEDELQIPV KWIGVGKSRE

SMIQLF
Length:456
Mass (Da):50,156
Last modified:December 16, 2008 - v2
Checksum:iFC588A4457986CB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC151541 mRNA. Translation: AAI51542.1.
RefSeqiNP_001161281.1. NM_001167809.1.
UniGeneiBt.24907.

Genome annotation databases

EnsembliENSBTAT00000047061; ENSBTAP00000044296; ENSBTAG00000013885.
GeneIDi522529.
KEGGibta:522529.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC151541 mRNA. Translation: AAI51542.1.
RefSeqiNP_001161281.1. NM_001167809.1.
UniGeneiBt.24907.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000044296.

Proteomic databases

PaxDbiA7MBG0.
PRIDEiA7MBG0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000047061; ENSBTAP00000044296; ENSBTAG00000013885.
GeneIDi522529.
KEGGibta:522529.

Organism-specific databases

CTDi159.

Phylogenomic databases

eggNOGiKOG1355. Eukaryota.
COG0104. LUCA.
GeneTreeiENSGT00390000015553.
HOGENOMiHOG000260959.
HOVERGENiHBG053768.
InParanoidiA7MBG0.
KOiK01939.
OMAiVQVKYEV.
OrthoDBiEOG7M6D76.
TreeFamiTF300486.

Enzyme and pathway databases

UniPathwayiUPA00075; UER00335.
ReactomeiR-BTA-73817. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

NextBioi20873548.

Family and domain databases

HAMAPiMF_00011. Adenylosucc_synth.
MF_03127. Adenylosucc_synth_vert_acid.
InterProiIPR018220. Adenylosuccin_syn_GTP-bd.
IPR033128. Adenylosuccin_syn_Lys_AS.
IPR001114. Adenylosuccinate_synthetase.
IPR027529. AdSS_2_vert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR11846. PTHR11846. 1 hit.
PfamiPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTiSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00184. purA. 1 hit.
PROSITEiPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Kidney.

Entry informationi

Entry nameiPURA2_BOVIN
AccessioniPrimary (citable) accession number: A7MBG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: December 16, 2008
Last modified: April 13, 2016
This is version 64 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.