Reviewed, UniProtKB/Swiss-Prot A7MBG0 (PURA2_BOVIN)

Names and origin

Protein names

Recommended name:
    Adenylosuccinate synthetase isozyme 2
      Short name=AMPSase 2
      Short name=AdSS 2
    EC=6.3.4.4
Alternative name(s):
    Adenylosuccinate synthetase, acidic isozyme
    IMP--aspartate ligase 2

Gene names

Name:

ADSS

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	456 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays an important role in the de novo pathway of purine nucleotide biosynthesis By similarity.
Catalytic activity	GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenylosuccinate synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylosuccinate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 456	456	Adenylosuccinate synthetase isozyme 2		PRO_0000355565
Regions
	Nucleotide binding	39 – 45	7	GTP Potential
Sites
	Active site	173	1	By similarity
	Active site	180	1	By similarity
	Metal binding	40	1	Magnesium By similarity
	Metal binding	67	1	Magnesium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MBG0-1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: FC588A4457986CB9	FASTA	456	50,156
	10 20 30 40 50 60 MAFAETNPAT SSLPNGDCGR PRTRPGGNRV TVVLGAQWGD EGKGKVVDLL AQDADIVCRC 70 80 90 100 110 120 QGGNNAGHTV VVDSVEYDFH LLPSGIINPN VTAFIGNGVV IHLPGLFEEA EKNVQKGKGL 130 140 150 160 170 180 EGWEKRLIIS DRAHIVFDFH QAADGIQEQQ RQEQAGKNLG TTKKGIGPVY SSKAARSGLR 190 200 210 220 230 240 MCDLVSDFGG FSERFKVLAN QYKSIYPTLE IDIEGELQKL KGYMERIKPM VRDGVYFLYE 250 260 270 280 290 300 ALHGPPKKIL VEGANAALLD IDFGTYPFVT SSNCTVGGVC TGLGMPPQNV GEVYGVVKAY 310 320 330 340 350 360 TTRVGIGAFP TEQDNEIGEL LQTRGREFGV TTGRKRRCGW LDLVLLKYAH MINGFTALAL 370 380 390 400 410 420 TKLDILDMFT EIKVGVAYKL DGEIIPHFPA NQEVLNKVEV QYKTLPGWNT DISNARTFKE 430 440 450 LPINAQNYVR FIEDELQIPV KWIGVGKSRE SMIQLF

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC151541 mRNA. Translation: AAI51542.1.
IPI	IPI00839758.
UniGene	Bt.24907
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A7MBG0.
Genome annotation databases
Ensembl	ENSBTAT00000047061; ENSBTAP00000044296; ENSBTAG00000013885; Bos taurus. [Genome view]
Family and domain databases
InterPro	IPR018220. Adenylosuccinate_synthase_AS. IPR001114. Adenylosuccinate_synthetase. [Graphical view]
PANTHER	PTHR11846. Asucc_synthtase. 1 hit.
Pfam	PF00709. Adenylsucc_synt. 1 hit. [Graphical view]
ProDom	PD001188. Asucc_synthtase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00788. Adenylsucc_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00184. purA. 1 hit.
PROSITE	PS01266. ADENYLOSUCCIN_SYN_1. 1 hit. PS00513. ADENYLOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PURA2_BOVIN

Accession

Primary (citable) accession number: A7MBG0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	December 16, 2008
Last modified:	October 13, 2009
This is version 17 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)