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Protein

Adenylosuccinate synthetase isozyme 2

Gene

ADSS

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Plays an important role in the de novo pathway and in the salvage pathway of purine nucleotide biosynthesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP (By similarity).By similarity

Catalytic activityi

GTP + IMP + L-aspartate = GDP + phosphate + N6-(1,2-dicarboxyethyl)-AMP.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: AMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from IMP.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Adenylosuccinate synthetase isozyme 2 (ADSS), Adenylosuccinate synthetase isozyme 1 (ADSSL1)
  2. Adenylosuccinate lyase (ADSL), Adenylosuccinate lyase (ADSL)
This subpathway is part of the pathway AMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from IMP, the pathway AMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei40Proton acceptorUniRule annotation1
Metal bindingi40MagnesiumUniRule annotation1
Binding sitei40SubstrateUniRule annotation1
Metal bindingi67Magnesium; via carbonyl oxygenUniRule annotation1
Active sitei68Proton donorUniRule annotation1
Binding sitei162IMPUniRule annotation1
Binding sitei176IMP; shared with dimeric partnerUniRule annotation1
Binding sitei255IMPUniRule annotation1
Binding sitei270IMPUniRule annotation1
Binding sitei334IMPUniRule annotation1
Binding sitei336GTPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi39 – 45GTPUniRule annotation7
Nucleotide bindingi67 – 69GTPUniRule annotation3
Nucleotide bindingi362 – 364GTPUniRule annotation3
Nucleotide bindingi444 – 447GTPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandGTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00075; UER00335

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylosuccinate synthetase isozyme 2UniRule annotation (EC:6.3.4.4UniRule annotation)
Short name:
AMPSase 2UniRule annotation
Short name:
AdSS 2UniRule annotation
Alternative name(s):
Adenylosuccinate synthetase, acidic isozymeUniRule annotation
Adenylosuccinate synthetase, liver isozymeUniRule annotation
Short name:
L-type adenylosuccinate synthetaseUniRule annotation
IMP--aspartate ligase 2UniRule annotation
Gene namesi
Name:ADSSUniRule annotation
Synonyms:ADSS2UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 16

Organism-specific databases

VGNCiVGNC:25700 ADSS

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003555651 – 456Adenylosuccinate synthetase isozyme 2Add BLAST456

Proteomic databases

PaxDbiA7MBG0
PRIDEiA7MBG0

Expressioni

Gene expression databases

BgeeiENSBTAG00000013885

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000044296

Structurei

3D structure databases

SMRiA7MBG0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni40 – 43IMP bindingUniRule annotation4
Regioni65 – 68IMP bindingUniRule annotation4
Regioni330 – 336Substrate bindingUniRule annotation7

Sequence similaritiesi

Belongs to the adenylosuccinate synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG1355 Eukaryota
COG0104 LUCA
GeneTreeiENSGT00390000015553
HOGENOMiHOG000260959
HOVERGENiHBG053768
InParanoidiA7MBG0
KOiK01939
OMAiGIAPFYS
OrthoDBiEOG091G1BIK
TreeFamiTF300486

Family and domain databases

CDDicd03108 AdSS, 1 hit
HAMAPiMF_00011 Adenylosucc_synth, 1 hit
MF_03127 Adenylosucc_synth_vert_acid, 1 hit
InterProiView protein in InterPro
IPR018220 Adenylosuccin_syn_GTP-bd
IPR033128 Adenylosuccin_syn_Lys_AS
IPR001114 Adenylosuccinate_synthetase
IPR027529 AdSS_2_vert
IPR027417 P-loop_NTPase
PANTHERiPTHR11846 PTHR11846, 1 hit
PfamiView protein in Pfam
PF00709 Adenylsucc_synt, 1 hit
SMARTiView protein in SMART
SM00788 Adenylsucc_synt, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00184 purA, 1 hit
PROSITEiView protein in PROSITE
PS01266 ADENYLOSUCCIN_SYN_1, 1 hit
PS00513 ADENYLOSUCCIN_SYN_2, 1 hit

Sequencei

Sequence statusi: Complete.

A7MBG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFAETNPAT SSLPNGDCGR PRTRPGGNRV TVVLGAQWGD EGKGKVVDLL
60 70 80 90 100
AQDADIVCRC QGGNNAGHTV VVDSVEYDFH LLPSGIINPN VTAFIGNGVV
110 120 130 140 150
IHLPGLFEEA EKNVQKGKGL EGWEKRLIIS DRAHIVFDFH QAADGIQEQQ
160 170 180 190 200
RQEQAGKNLG TTKKGIGPVY SSKAARSGLR MCDLVSDFGG FSERFKVLAN
210 220 230 240 250
QYKSIYPTLE IDIEGELQKL KGYMERIKPM VRDGVYFLYE ALHGPPKKIL
260 270 280 290 300
VEGANAALLD IDFGTYPFVT SSNCTVGGVC TGLGMPPQNV GEVYGVVKAY
310 320 330 340 350
TTRVGIGAFP TEQDNEIGEL LQTRGREFGV TTGRKRRCGW LDLVLLKYAH
360 370 380 390 400
MINGFTALAL TKLDILDMFT EIKVGVAYKL DGEIIPHFPA NQEVLNKVEV
410 420 430 440 450
QYKTLPGWNT DISNARTFKE LPINAQNYVR FIEDELQIPV KWIGVGKSRE

SMIQLF
Length:456
Mass (Da):50,156
Last modified:December 16, 2008 - v2
Checksum:iFC588A4457986CB9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC151541 mRNA Translation: AAI51542.1
RefSeqiNP_001161281.1, NM_001167809.1
UniGeneiBt.24907

Genome annotation databases

EnsembliENSBTAT00000047061; ENSBTAP00000044296; ENSBTAG00000013885
GeneIDi522529
KEGGibta:522529

Similar proteinsi

Entry informationi

Entry nameiPURA2_BOVIN
AccessioniPrimary (citable) accession number: A7MBG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 16, 2008
Last sequence update: December 16, 2008
Last modified: March 28, 2018
This is version 72 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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