Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A7MBD1

- MPIP1_BOVIN

UniProt

A7MBD1 - MPIP1_BOVIN

Protein

M-phase inducer phosphatase 1

Gene

CDC25A

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (02 Oct 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro By similarity.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Enzyme regulationi

    Stimulated by B-type cyclins. Stimulated by PIM1-mediated phosphorylation.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei432 – 4321By similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular response to UV Source: Ensembl
    2. mitotic nuclear division Source: UniProtKB-KW
    3. response to radiation Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_203965. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_206864. Cyclin A:Cdk2-associated events at S phase entry.
    REACT_215433. Cyclin B2 mediated events.
    REACT_222889. E2F-enabled inhibition of pre-replication complex formation.
    REACT_224007. Cyclin A/B1 associated events during G2/M transition.
    REACT_226948. G0 and Early G1.
    REACT_227227. p53-Independent DNA Damage Response.
    REACT_227740. Activation of ATR in response to replication stress.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    M-phase inducer phosphatase 1 (EC:3.1.3.48)
    Alternative name(s):
    Dual specificity phosphatase Cdc25A
    Gene namesi
    Name:CDC25A
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 22

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleus Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 525525M-phase inducer phosphatase 1PRO_0000365161Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei75 – 751Phosphoserine; by CHEK1By similarity
    Modified residuei78 – 781Phosphoserine; by NEK11By similarity
    Modified residuei81 – 811Phosphoserine; by NEK11By similarity
    Modified residuei87 – 871Phosphoserine; by NEK11By similarity
    Modified residuei123 – 1231Phosphoserine; by CHEK1 and CHEK2By similarity
    Modified residuei177 – 1771Phosphoserine; by CHEK1By similarity
    Modified residuei279 – 2791Phosphoserine; by CHEK1 and CHEK2By similarity
    Modified residuei293 – 2931Phosphoserine; by CHEK1 and CHEK2By similarity
    Modified residuei508 – 5081Phosphothreonine; by CHEK1By similarity
    Modified residuei514 – 5141Phosphoserine; by PLK3By similarity
    Modified residuei520 – 5201Phosphoserine; by PLK3By similarity

    Post-translational modificationi

    Phosphorylated by CHEK1 on Ser-75, Ser-123, Ser-177, Ser-279, Ser-293 and Thr-508 during checkpoint mediated cell cycle arrest. Also phosphorylated by CHEK2 on Ser-123, Ser-279, and Ser-293 during checkpoint mediated cell cycle arrest. Phosphorylation on Ser-177 and Thr-508 creates binding sites for YWHAE/14-3-3 epsilon which inhibits CDC25A. Phosphorylation on Ser-75, Ser-123, Ser-177, Ser-279 and Ser-293 may also promote ubiquitin-dependent proteolysis of CDC25A by the SCF complex. Phosphorylation of CDC25A at Ser-75 by CHEK1 primes it for subsequent phosphorylation at Ser-78, Ser-81 and Ser-87 by NEK11. Phosphorylation by NEK11 is required for BTRC-mediated polyubiquitination and degradation. Phosphorylation by PIM1 leads to an increase in phosphatase activity. Phosphorylated by PLK3 following DNA damage, leading to promote its ubiquitination and degradation By similarity.By similarity
    Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) ubiquitin ligase complex that contains FZR1/CDH1 during G1 phase leading to its degradation by the proteasome. Ubiquitinated by a SCF complex containing BTRC and FBXW11 during S phase leading to its degradation by the proteasome. Deubiquitination by USP17L2/DUB3 leads to its stabilization By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PRIDEiA7MBD1.

    Interactioni

    Subunit structurei

    Interacts with CCNB1/cyclin B1. Interacts with YWHAE/14-3-3 epsilon when phosphorylated. Interacts with CUL1 specifically when CUL1 is neddylated and active. Interacts with BTRC/BTRCP1 and FBXW11/BTRCP2. Interactions with CUL1, BTRC and FBXW11 are enhanced upon DNA damage By similarity. Interacts with PIM1 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000012611.

    Structurei

    3D structure databases

    ProteinModelPortaliA7MBD1.
    SMRiA7MBD1. Positions 338-497.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini377 – 483107RhodanesePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi73 – 8311PhosphodegronAdd
    BLAST
    Motifi140 – 1423KEN box

    Domaini

    The phosphodegron motif mediates interaction with specific F-box proteins when phosphorylated. Putative phosphorylation sites at Ser-78 and Ser-81 appear to be essential for this interaction By similarity.By similarity

    Sequence similaritiesi

    Belongs to the MPI phosphatase family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5105.
    GeneTreeiENSGT00390000018747.
    HOGENOMiHOG000082672.
    HOVERGENiHBG052501.
    InParanoidiA7MBD1.
    KOiK06645.
    OMAiNLTVTMD.
    OrthoDBiEOG7288R1.
    TreeFamiTF101056.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF06617. M-inducer_phosp. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view]
    PRINTSiPR00716. MPIPHPHTASE.
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7MBD1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELGPEPPHR RRLLFACSPP PAPQPVVKAL FGTPAAGGLS PVTNLTVTMD    50
    QLQGLGSEYE QPIEVKNSSL QRMGSSESTD SGFCLDSPGP LDSKENLENP 100
    MRRINSLPQK LLGCSPALKR SHSDSLDHDV FQLIDQDENK ENQVFEFKKP 150
    IRPASRGCLH VHGLEEGKDV FTQRQNSAPA RMLSSNERDG NEPGNSIPFM 200
    PQSPVTPTLS DEDDGFMDLL DGENLKNDEE TPSCMASLWT APLVMRRTNN 250
    LGNRCKLFDS PSASCSSTIR SMLKRPDRSL EESPGGSRKR RKSVAGASPE 300
    EAASPEKPQE ILHHQSLSLA SSPKGTIENI LDNDPRDLIG DFSKGYLFHT 350
    VAGKHQDLKY ISPEIITSVL NGKFANLIKE FVIIDCRYPY EYEGGHIKGA 400
    VNLHMEEEVE EFLLKKPIVP TDGKRVIVVF HCEFSSERGP RMCRYVRERD 450
    RLGNEYPKLH YPELYVLKGG YKEFFLKCQS HCEPPSYRPM HHEDFKEDLK 500
    KFRTKSRTWA GEKSKREMYS RLKKL 525
    Length:525
    Mass (Da):59,142
    Last modified:October 2, 2007 - v1
    Checksum:i3608ED70EE8293BB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC151493 mRNA. Translation: AAI51494.1.
    RefSeqiNP_001094570.1. NM_001101100.2.
    UniGeneiBt.62587.

    Genome annotation databases

    EnsembliENSBTAT00000012611; ENSBTAP00000012611; ENSBTAG00000009586.
    GeneIDi520188.
    KEGGibta:520188.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC151493 mRNA. Translation: AAI51494.1 .
    RefSeqi NP_001094570.1. NM_001101100.2.
    UniGenei Bt.62587.

    3D structure databases

    ProteinModelPortali A7MBD1.
    SMRi A7MBD1. Positions 338-497.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000012611.

    Proteomic databases

    PRIDEi A7MBD1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000012611 ; ENSBTAP00000012611 ; ENSBTAG00000009586 .
    GeneIDi 520188.
    KEGGi bta:520188.

    Organism-specific databases

    CTDi 993.

    Phylogenomic databases

    eggNOGi COG5105.
    GeneTreei ENSGT00390000018747.
    HOGENOMi HOG000082672.
    HOVERGENi HBG052501.
    InParanoidi A7MBD1.
    KOi K06645.
    OMAi NLTVTMD.
    OrthoDBi EOG7288R1.
    TreeFami TF101056.

    Enzyme and pathway databases

    Reactomei REACT_203965. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
    REACT_206864. Cyclin A:Cdk2-associated events at S phase entry.
    REACT_215433. Cyclin B2 mediated events.
    REACT_222889. E2F-enabled inhibition of pre-replication complex formation.
    REACT_224007. Cyclin A/B1 associated events during G2/M transition.
    REACT_226948. G0 and Early G1.
    REACT_227227. p53-Independent DNA Damage Response.
    REACT_227740. Activation of ATR in response to replication stress.

    Miscellaneous databases

    NextBioi 20873047.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view ]
    Pfami PF06617. M-inducer_phosp. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PRINTSi PR00716. MPIPHPHTASE.
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal liver.

    Entry informationi

    Entry nameiMPIP1_BOVIN
    AccessioniPrimary (citable) accession number: A7MBD1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 3, 2009
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3