A7MBB4 (M3K13_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase kinase kinase 13
EC=2.7.11.25

Gene names

Name:

MAP3K13

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	966 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Activates the JUN N-terminal pathway through activation of the MAP kinase kinase MAP2K7. Acts synergistically with PRDX3 to regulate the activation of NF-kappa-B in the cytosol. This activation is kinase-dependent and involves activating the IKK complex, the IKBKB-containing complex that phosphorylates inhibitors of NF-kappa-B By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by autophosphorylation and homodimerization By similarity.
Subunit structure	Homodimer; forms dimers through the leucine-zipper motif. Interacts with the C-terminus of MAPK8IP1 through the kinase catalytic domain. Binds PRDX3. Associates with the IKK complex through the kinase domain By similarity.
Subcellular location	Cytoplasm. Membrane; Peripheral membrane protein By similarity.
Post-translational modification	Autophosphorylated on serine and threonine residues By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Domain	Coiled coil Repeat
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein autophosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 966	966	Mitogen-activated protein kinase kinase kinase 13		PRO_0000366128
Regions
	Domain	168 – 409	242	Protein kinase
	Domain	433 – 454	22	Leucine-zipper 1
	Domain	486 – 507	22	Leucine-zipper 2
	Nucleotide binding	174 – 182	9	ATP By similarity
	Region	815 – 828	14	Acidic By similarity
	Coiled coil	457 – 496	40	Potential
	Compositional bias	576 – 582	7	Poly-Ser
	Compositional bias	817 – 822	6	Poly-Glu
Sites
	Active site	279	1	Proton acceptor By similarity
	Binding site	195	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7MBB4 [UniParc]. Last modified October 2, 2007. Version 1. Checksum: 4D632EAC6AF13FF5	FASTA	966	108,152
	10 20 30 40 50 60 MANPQEHLSC SSSPRLPLSE NKTFNGLQDD LAPMGSHASP KLLKDQQEKG MVQTELAEGT 70 80 90 100 110 120 NSPITTTVLT SISEDSRDQF ENSVLQLREQ DESEMAMSHG NSNTVDGEGT SGTEDIKIQF 130 140 150 160 170 180 SRSGSGSGGF LEGLFGCLRP VWNIIGKAYS TDYKLQQQDT WEVPFEEISE LQWLGSGAQG 190 200 210 220 230 240 AVFLGKFRAE EVAIKKVREQ NETDIKHLRK LKHPNIIAFK GVCTQAPCYC IIMEYCAHGQ 250 260 270 280 290 300 LYEVLRAGRK ITPRLLVDWS TGIASGMNYL HLHKIIHRDL KSPNVLVTHT DAVKISDFGT 310 320 330 340 350 360 SKELSDKSTK MSFAGTVAWM APEVIRNEPV SEKVDIWSFG VVLWELLTGE IPYKDVDSSA 370 380 390 400 410 420 IIWGVGSNSL HLPVPSTCPD GFKILMKQTW QSKTRNRPSF RQTLMHLDIA SADVLATPQE 430 440 450 460 470 480 TYFKSQAEWR EEVKKHFEKI KSEGTCIHRL DEELIRRRRE ELRHALDIRE HYERKLERAN 490 500 510 520 530 540 NLYMELSAIM LQLEMREKEL IKREQAVEKK YPGTYKRHPV RPIIHPNAME KLMKRKGMPH 550 560 570 580 590 600 RPGMQAKRPD LLRSEGIPSV EVAPTASPLS GSPKLSSSSS KSRYRSKPRH RRGNSRGSHG 610 620 630 640 650 660 DFAAILKNQP AQEDSPHPTS LHQAEPQYPS SQHHNLLQQQ YQQPPPAMSQ SHHPRLNMHG 670 680 690 700 710 720 QDIATCPNNL RYFGPAAALR SPLSNHSQRQ MPGSSPDLIS TAMAADCWRS SEPDKGQAGP 730 740 750 760 770 780 WGCCQADPYD PCLQCRPEQH GSLDVPSAKP VGRSPSLFKP PAHNPLLENA QGSEKMEENE 790 800 810 820 830 840 FSGYRSASSL GASHHITPPV LPRKTRPLQK SGDDSSEEEE GEVDSEVEFP RRQRPHRCIS 850 860 870 880 890 900 SCQSYSTFSS ENFSVSDGEE GNTSDHSNSP DELADKLEDH LAEKLDDLLS QTPEIPIEIS 910 920 930 940 950 960 SHSDGLSDKE CAVRRVKTQM SLGKLCAEER GYENPMQFEE SDCDSSDGEC SDATVRTNKH YSSATW

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC151464 mRNA. Translation: AAI51465.1.
IPI	IPI00702547.
RefSeq	NP_001095323.1. NM_001101853.1.
UniGene	Bt.78411.
3D structure databases
ProteinModelPortal	A7MBB4.
ModBase	Search...
Protein-protein interaction databases
STRING	A7MBB4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	505369.
KEGG	bta:505369.
Organism-specific databases
CTD	9175.
Phylogenomic databases
eggNOG	maNOG11674.
GeneTree	ENSGT00600000084040.
HOVERGEN	HBG052383.
InParanoid	A7MBB4.
OrthoDB	EOG45MN4T.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR017419. MAP3K12_MAP3K13. IPR000719. Prot_kinase_cat_dom. IPR001245. Ser-Thr/Tyr_kinase. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
KO	K04422.
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PIRSF	PIRSF038165. MAPKKK12_MAPKKK13. 1 hit.
PRINTS	PR00109. TYRKINASE.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

M3K13_BOVIN

Accession

Primary (citable) accession number: A7MBB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	October 2, 2007
Last modified:	November 16, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program