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A7MB62

- ARP2_BOVIN

UniProt

A7MB62 - ARP2_BOVIN

Protein

Actin-related protein 2

Gene

ACTR2

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi160 – 1623ATP
    Nucleotide bindingi214 – 2185ATP
    Nucleotide bindingi305 – 3106ATP

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct

    Keywords - Ligandi

    Actin-binding, ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_196755. Regulation of actin dynamics for phagocytic cup formation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Actin-related protein 2
    Alternative name(s):
    Actin-like protein 2
    Gene namesi
    Name:ACTR2
    Synonyms:ARP2
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    Cytoplasmcytoskeleton By similarity. Cell projection By similarity

    GO - Cellular componenti

    1. cell projection Source: UniProtKB-SubCell
    2. cytoskeleton Source: UniProtKB-SubCell
    3. cytosol Source: Reactome

    Keywords - Cellular componenti

    Cell projection, Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 394394Actin-related protein 2PRO_0000327246Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei299 – 2991N6-acetyllysineBy similarity
    Modified residuei322 – 3221N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiA7MB62.
    PRIDEiA7MB62.

    Interactioni

    Subunit structurei

    Component of the Arp2/3 complex composed of ARP2, ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    WASLQ951072EBI-6162748,EBI-6162776

    Protein-protein interaction databases

    DIPiDIP-29789N.
    IntActiA7MB62. 2 interactions.
    STRINGi9913.ENSBTAP00000012872.

    Structurei

    Secondary structure

    1
    394
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi10 – 134
    Beta strandi15 – 217
    Beta strandi29 – 335
    Beta strandi36 – 405
    Helixi59 – 635
    Helixi65 – 673
    Beta strandi68 – 714
    Beta strandi73 – 753
    Helixi84 – 9310
    Turni95 – 984
    Helixi102 – 1043
    Beta strandi109 – 1113
    Turni117 – 1193
    Beta strandi134 – 1407
    Helixi141 – 1477
    Turni148 – 1503
    Beta strandi156 – 1594
    Beta strandi164 – 1674
    Beta strandi171 – 1744
    Turni176 – 1783
    Beta strandi180 – 1834
    Helixi186 – 19914
    Turni206 – 2094
    Helixi210 – 22011
    Helixi227 – 23610
    Beta strandi238 – 2403
    Beta strandi242 – 2454
    Beta strandi251 – 2544
    Helixi257 – 2604
    Helixi262 – 2654
    Helixi268 – 2714
    Helixi278 – 28811
    Turni291 – 2933
    Helixi294 – 2985
    Beta strandi302 – 3054
    Helixi306 – 3083
    Helixi313 – 32816
    Turni329 – 3324
    Helixi336 – 3394
    Helixi350 – 3523
    Helixi353 – 3619
    Turni365 – 3673
    Beta strandi369 – 3746
    Helixi375 – 3795

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1K8KX-ray2.00B1-394[»]
    1TYQX-ray2.55B1-394[»]
    1U2VX-ray2.55B1-394[»]
    2P9IX-ray2.46B1-394[»]
    2P9KX-ray2.59B1-394[»]
    2P9LX-ray2.65B1-394[»]
    2P9NX-ray2.85B1-394[»]
    2P9PX-ray2.90B1-394[»]
    2P9SX-ray2.68B1-394[»]
    2P9UX-ray2.75B1-394[»]
    3DXKX-ray2.70B1-394[»]
    3DXMX-ray2.85B1-394[»]
    3RSEX-ray2.65B1-394[»]
    3UKRX-ray2.48B1-394[»]
    3UKUX-ray2.75B1-394[»]
    3ULEX-ray2.50B1-394[»]
    4JD2X-ray3.08B1-394[»]
    ProteinModelPortaliA7MB62.
    SMRiA7MB62. Positions 4-388.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiA7MB62.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the actin family. ARP2 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG5277.
    HOGENOMiHOG000233340.
    HOVERGENiHBG003771.
    InParanoidiA7MB62.
    KOiK17260.

    Family and domain databases

    InterProiIPR004000. Actin-related.
    IPR020902. Actin/actin-like_CS.
    [Graphical view]
    PANTHERiPTHR11937. PTHR11937. 1 hit.
    PfamiPF00022. Actin. 1 hit.
    [Graphical view]
    PRINTSiPR00190. ACTIN.
    SMARTiSM00268. ACTIN. 1 hit.
    [Graphical view]
    PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7MB62-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDSQGRKVVV CDNGTGFVKC GYAGSNFPEH IFPALVGRPI IRSTTKVGNI    50
    EIKDLMVGDE ASELRSMLEV NYPMENGIVR NWDDMKHLWD YTFGPEKLNI 100
    DTRNCKILLT EPPMNPTKNR EKIVEVMFET YQFSGVYVAI QAVLTLYAQG 150
    LLTGVVVDSG DGVTHICPVY EGFSLPHLTR RLDIAGRDIT RYLIKLLLLR 200
    GYAFNHSADF ETVRMIKEKL CYVGYNIEQE QKLALETTVL VESYTLPDGR 250
    IIKVGGERFE APEALFQPHL INVEGVGVAE LLFNTIQAAD IDTRSEFYKH 300
    IVLSGGSTMY PGLPSRLERE LKQLYLERVL KGDVEKLSKF KIRIEDPPRR 350
    KHMVFLGGAV LADIMKDKDN FWMTRQEYQE KGVRVLEKLG VTVR 394
    Length:394
    Mass (Da):44,761
    Last modified:October 2, 2007 - v1
    Checksum:i1BFA6B442ED1A797
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC151356 mRNA. Translation: AAI51357.1.
    RefSeqiNP_001095683.1. NM_001102213.1.
    UniGeneiBt.3684.

    Genome annotation databases

    GeneIDi538486.
    KEGGibta:538486.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC151356 mRNA. Translation: AAI51357.1 .
    RefSeqi NP_001095683.1. NM_001102213.1.
    UniGenei Bt.3684.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1K8K X-ray 2.00 B 1-394 [» ]
    1TYQ X-ray 2.55 B 1-394 [» ]
    1U2V X-ray 2.55 B 1-394 [» ]
    2P9I X-ray 2.46 B 1-394 [» ]
    2P9K X-ray 2.59 B 1-394 [» ]
    2P9L X-ray 2.65 B 1-394 [» ]
    2P9N X-ray 2.85 B 1-394 [» ]
    2P9P X-ray 2.90 B 1-394 [» ]
    2P9S X-ray 2.68 B 1-394 [» ]
    2P9U X-ray 2.75 B 1-394 [» ]
    3DXK X-ray 2.70 B 1-394 [» ]
    3DXM X-ray 2.85 B 1-394 [» ]
    3RSE X-ray 2.65 B 1-394 [» ]
    3UKR X-ray 2.48 B 1-394 [» ]
    3UKU X-ray 2.75 B 1-394 [» ]
    3ULE X-ray 2.50 B 1-394 [» ]
    4JD2 X-ray 3.08 B 1-394 [» ]
    ProteinModelPortali A7MB62.
    SMRi A7MB62. Positions 4-388.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-29789N.
    IntActi A7MB62. 2 interactions.
    STRINGi 9913.ENSBTAP00000012872.

    Proteomic databases

    PaxDbi A7MB62.
    PRIDEi A7MB62.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 538486.
    KEGGi bta:538486.

    Organism-specific databases

    CTDi 10097.

    Phylogenomic databases

    eggNOGi COG5277.
    HOGENOMi HOG000233340.
    HOVERGENi HBG003771.
    InParanoidi A7MB62.
    KOi K17260.

    Enzyme and pathway databases

    Reactomei REACT_196755. Regulation of actin dynamics for phagocytic cup formation.

    Miscellaneous databases

    EvolutionaryTracei A7MB62.
    NextBioi 20877389.

    Family and domain databases

    InterProi IPR004000. Actin-related.
    IPR020902. Actin/actin-like_CS.
    [Graphical view ]
    PANTHERi PTHR11937. PTHR11937. 1 hit.
    Pfami PF00022. Actin. 1 hit.
    [Graphical view ]
    PRINTSi PR00190. ACTIN.
    SMARTi SM00268. ACTIN. 1 hit.
    [Graphical view ]
    PROSITEi PS01132. ACTINS_ACT_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Fetal skin.
    2. Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF ARP2/3 COMPLEX.
    3. "Crystal structures of actin-related protein 2/3 complex with bound ATP or ADP."
      Nolen B.J., Littlefield R.S., Pollard T.D.
      Proc. Natl. Acad. Sci. U.S.A. 101:15627-15632(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.55 ANGSTROMS) OF ARP2/3 COMPLEX WITH BOUND ATP.
    4. "Insights into the influence of nucleotides on actin family proteins from seven structures of Arp2/3 complex."
      Nolen B.J., Pollard T.D.
      Mol. Cell 26:449-457(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.46 ANGSTROMS) OF ARP2/3 COMPLEX WITH BOUND ATP.

    Entry informationi

    Entry nameiARP2_BOVIN
    AccessioniPrimary (citable) accession number: A7MB62
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3