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Protein

Eukaryotic translation initiation factor 3 subunit B

Gene

EIF3B

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-BTA-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-BTA-72649. Translation initiation complex formation.
R-BTA-72689. Formation of a pool of free 40S subunits.
R-BTA-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-BTA-72702. Ribosomal scanning and start codon recognition.
R-BTA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit BUniRule annotation
Short name:
eIF3bUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 9UniRule annotation
eIF-3-etaUniRule annotation
Gene namesi
Name:EIF3BUniRule annotation
Synonyms:EIF3S9UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 25

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 786786Eukaryotic translation initiation factor 3 subunit BPRO_0000363786Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineUniRule annotationBy similarity
Modified residuei104 – 1041PhosphoserineUniRule annotationBy similarity
Modified residuei124 – 1241PhosphoserineUniRule annotationBy similarity
Modified residuei126 – 1261PhosphoserineUniRule annotationBy similarity
Modified residuei136 – 1361PhosphoserineUniRule annotationBy similarity
Modified residuei181 – 1811N6-acetyllysineBy similarity
Modified residuei211 – 2111PhosphoserineUniRule annotationBy similarity
Modified residuei260 – 2601N6-acetyllysineBy similarity
Modified residuei336 – 3361N6-acetyllysineBy similarity

Post-translational modificationi

Phosphorylated. Phosphorylation is enhanced upon serum stimulation.UniRule annotation

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiA7MB16.
PRIDEiA7MB16.

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Also interacts with UPF2 Also interacts with UPF2 and HNRPD (By similarity).UniRule annotation

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009832.

Structurei

3D structure databases

ProteinModelPortaliA7MB16.
SMRiA7MB16. Positions 142-246.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini157 – 24084RRMUniRule annotationAdd
BLAST
Repeati304 – 34239WD 1Add
BLAST
Repeati344 – 38946WD 2Add
BLAST
Repeati393 – 43139WD 3Add
BLAST
Repeati532 – 57342WD 4Add
BLAST
Repeati621 – 66646WD 5Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni96 – 385290Sufficient for interaction with EIF3EUniRule annotationAdd
BLAST
Regioni142 – 246105Sufficient for interaction with EIF3JUniRule annotationAdd
BLAST

Domaini

The RRM domain mediates interaction with EIF3J.UniRule annotation

Sequence similaritiesi

Belongs to the eIF-3 subunit B family.UniRule annotation
Contains 1 RRM (RNA recognition motif) domain.UniRule annotation
Contains 5 WD repeats.UniRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG2314. Eukaryota.
COG5354. LUCA.
GeneTreeiENSGT00550000074913.
HOGENOMiHOG000265546.
HOVERGENiHBG006127.
InParanoidiA7MB16.
KOiK03253.
OMAiDYQEEPN.
OrthoDBiEOG7KM5S6.
TreeFamiTF101521.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.130.10.10. 1 hit.
3.30.70.330. 1 hit.
HAMAPiMF_03001. eIF3b.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR011400. EIF3B.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR14068. PTHR14068. 1 hit.
PfamiPF08662. eIF2A. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036424. eIF3b. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7MB16-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDAENVAAP EAAEQRAEPG PEQAAAEPSP GAEVARPGVQ EAAGGEDAEA
60 70 80 90 100
GPGPEGPAEP AADGEGKADA TPGATPPPPE ESSAQLAGEA PAEQAQDAAA
110 120 130 140 150
EAGSEGAGGD PDGAAEDGGA DEPSFSDPED FVDDVSEEEL LADVLKDRPQ
160 170 180 190 200
EADGIDSVIV VDNVPQVGPD RLEKLKNVIH KIFSKFGKIT NDFYPEEDGR
210 220 230 240 250
TKGYIFLEYA SPAHALDAVK NADGYKLDKQ HTFRVNLFTD FDKYMTISDE
260 270 280 290 300
WDIPEKQPFK DLGNLRYWLE EAECRDQYSV IFESGDRTSI FWNDVKDPVS
310 320 330 340 350
IEERARWTET YVRWSPKGTY LATFHQRGIA LWGGEKFKQI QRFSHQGVQL
360 370 380 390 400
IDFSPCERYL VTFSPLMDTQ DDPQAIIIWD ILTGQKKRGF HCESSAHWPI
410 420 430 440 450
FKWSHDGKFF ARMTLDTLSI YETPSMGLLD KKSLKISGIK DFSWSPGGNI
460 470 480 490 500
IAFWVPEDKD IPARVTLMQL PTRQEIRVRN LFNVVDCKLH WQKNGDYLCV
510 520 530 540 550
KVDRTPKGTQ GVVTNFEIFR MREKQVPVDV VEMKETIIAF AWEPNGSKFA
560 570 580 590 600
VLHGEAPRIS VSFYHVKNNG KIELIKMFDK QQANTIFWSP QGQFVVLAGL
610 620 630 640 650
RSMNGALAFV DTSDCTVMNI AEHYMASDVE WDPTGRYVVT SVSWWSHKVD
660 670 680 690 700
NAYWLWTFQG RLLQKNSKDR FCQLLWRPRP PTLLSQDQIK QIKKDLKKYS
710 720 730 740 750
KIFEQKDRLS QSKASKELVE RRRTMMEDFR KYRKMAQELY MEQKNARLEL
760 770 780
RGGVDTDELD SNVDDWEEET IEFFVTEEII PLGNQE
Length:786
Mass (Da):88,919
Last modified:October 2, 2007 - v1
Checksum:i27B9CA7AF11DF8D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC151284 mRNA. Translation: AAI51285.1.
RefSeqiNP_001095826.1. NM_001102356.1.
UniGeneiBt.55760.

Genome annotation databases

EnsembliENSBTAT00000009832; ENSBTAP00000009832; ENSBTAG00000007474.
GeneIDi789999.
KEGGibta:789999.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC151284 mRNA. Translation: AAI51285.1.
RefSeqiNP_001095826.1. NM_001102356.1.
UniGeneiBt.55760.

3D structure databases

ProteinModelPortaliA7MB16.
SMRiA7MB16. Positions 142-246.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000009832.

Proteomic databases

PaxDbiA7MB16.
PRIDEiA7MB16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000009832; ENSBTAP00000009832; ENSBTAG00000007474.
GeneIDi789999.
KEGGibta:789999.

Organism-specific databases

CTDi8662.

Phylogenomic databases

eggNOGiKOG2314. Eukaryota.
COG5354. LUCA.
GeneTreeiENSGT00550000074913.
HOGENOMiHOG000265546.
HOVERGENiHBG006127.
InParanoidiA7MB16.
KOiK03253.
OMAiDYQEEPN.
OrthoDBiEOG7KM5S6.
TreeFamiTF101521.

Enzyme and pathway databases

ReactomeiR-BTA-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-BTA-72649. Translation initiation complex formation.
R-BTA-72689. Formation of a pool of free 40S subunits.
R-BTA-72695. Formation of the ternary complex, and subsequently, the 43S complex.
R-BTA-72702. Ribosomal scanning and start codon recognition.
R-BTA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.

Miscellaneous databases

NextBioi20930006.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
2.130.10.10. 1 hit.
3.30.70.330. 1 hit.
HAMAPiMF_03001. eIF3b.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR011400. EIF3B.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR013979. TIF_beta_prop-like.
IPR015943. WD40/YVTN_repeat-like_dom.
[Graphical view]
PANTHERiPTHR14068. PTHR14068. 1 hit.
PfamiPF08662. eIF2A. 1 hit.
PF00076. RRM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF036424. eIF3b. 1 hit.
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. NIH - Mammalian Gene Collection (MGC) project
    Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Fetal skin.

Entry informationi

Entry nameiEIF3B_BOVIN
AccessioniPrimary (citable) accession number: A7MB16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 10, 2009
Last sequence update: October 2, 2007
Last modified: January 20, 2016
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.