Reviewed, UniProtKB/Swiss-Prot A7M993 (CYB6_CUSRE)

Names and origin

Protein names

Recommended name:
    Cytochrome b6

Gene names

Name:

petB

Encoded on

Plastid

Organism

Cuscuta reflexa (Southern Asian dodder)

Taxonomic identifier

4129 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Convolvulaceae › Cuscuteae › Cuscuta

Protein attributes

Sequence length	215 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity.
Cofactor	Binds 2 heme groups. One heme group is bound covalently by a single cysteine link, the other one non-covalently By similarity.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity.
Subcellular location	Plastid thylakoid membrane; Multi-pass membrane protein Potential.
Miscellaneous	Heme 1 (or BH or b566) is high-potential and absorbs at about 566 nm, and heme 2 (or BL or b562) is low-potential and absorbs at about 562 nm By similarity.
Sequence similarities	Belongs to the cytochrome b family. PetB subfamily.
Caution	Young tissue from this organism is photosynthetic and contains some thylakoids, although the photosynthetic activity does not exceed the light compensation point.
RNA editing	Edited at positions 140 and 204. Ref.1

Ontologies

Keywords
Biological process	Electron transport Photosynthesis Transport
Cellular component	Membrane Plastid Thylakoid
Coding sequence diversity	RNA editing
Domain	Transmembrane
Ligand	Heme Iron Metal-binding
Gene Ontology (GO)
Biological process	photosynthesis Inferred from electronic annotation. Source: HAMAP respiratory electron transport chain Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plastid thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 215	215	Cytochrome b6 HAMAP MF_00633		PRO_0000308481
Regions
	Transmembrane	32 – 52	21	Potential
	Transmembrane	90 – 110	21	Potential
	Transmembrane	116 – 136	21	Potential
	Transmembrane	186 – 206	21	Potential
Sites
	Metal binding	86	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	100	1	Iron (heme 1 axial ligand) By similarity
	Metal binding	187	1	Iron (heme 2 axial ligand) By similarity
	Metal binding	202	1	Iron (heme 1 axial ligand) By similarity
	Binding site	35	1	Heme 1 (covalent; via 1 link) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7M993-1 [UniParc]. Last modified October 23, 2007. Version 2. Checksum: A1F25C669E5408B2	FASTA	215	24,161
	10 20 30 40 50 60 MSKVYDWLEE RLEIQAIADD ITSKYVPPHV NIFYCLGGIT LTCFLVQVAT GFAMTFYYRP 70 80 90 100 110 120 TVTEAFASVK YIMTEANFGW LIRSVHRWSA SMMVLMMILH VFRVYLTGGF KKPRELTWVT 130 140 150 160 170 180 GVVLAVLTAS FGVTGYSLPW DQIGYWAVKI VTGVPEAIPL IGSPLVELLR GSASVGQSTL 190 200 210 TRFYSLHTFV LPLITAVFML MHFLMIRKQG ISGPL

References

[1]

"Complete DNA sequences of the plastid genomes of two parasitic flowering plant species, Cuscuta reflexa and Cuscuta gronovii." Funk H.T., Berg S., Krupinska K., Maier U.-G., Krause K. BMC Plant Biol. 7:45-45(2007) [PubMed: 17714582] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], RNA EDITING.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM711640 Genomic DNA. Translation: CAM98421.1. Sequence problems.
RefSeq	YP_001430134.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5536694.
Family and domain databases
HAMAP	MF_00633. [Tree]
InterPro	IPR016175. Cyt_b/b6. IPR005797. Cyt_b/b6_N. [Graphical view]
Gene3D	G3DSA:1.20.810.10. Cytochrome_b/b6. 1 hit.
PANTHER	PTHR19271. Cytochrome_b/b6. 1 hit.
Pfam	PF00033. Cytochrom_B_N. 1 hit. [Graphical view]
PROSITE	PS51002. CYTB_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYB6_CUSRE

Accession

Primary (citable) accession number: A7M993

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	October 23, 2007
Last modified:	March 3, 2009
This is version 13 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)