Reviewed, UniProtKB/Swiss-Prot A7KAN4 (ATG26_PENCW)

Names and origin

Protein names

Recommended name:
    Sterol 3-beta-glucosyltransferase
    EC=2.4.1.173
Alternative name(s):
    Autophagy-related protein 26

Gene names

Name:	atg26
ORF Names:	Pc12g16190

Organism

Penicillium chrysogenum (strain ATCC 28089 / DSM 1075 / Wisconsin 54-1255) (Penicillium notatum) [Complete proteome]

Taxonomic identifier

500485 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium › Penicillium chrysogenum complex

Protein attributes

Sequence length	1374 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity. Ref.1
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Cytoplasm Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1374	1374	Sterol 3-beta-glucosyltransferase		PRO_0000318045
Regions
	Domain	234 – 283	50	GRAM 1
	Domain	285 – 382	98	PH
	Domain	704 – 770	67	GRAM 2

Sequences

	Sequence		Length	Mass (Da)
	A7KAN4-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 1ED04830463732DF	FASTA	1,374	153,587
	10 20 30 40 50 60 MRPLRDDAKR RADRQLSASM KPTSSNRPFS DRVPDRFKDG DDAQFDFTAP PRGMGSRDGN 70 80 90 100 110 120 VHYMQQSLFS MIAAVGSKSD FHARFDESSD SDGEMEEQPR VEQTTGKASS RTPPVPSLDP 130 140 150 160 170 180 RQPSKRSEPS KLVLEERGRR HQRACSDNKL LQRLPKSDAE GGVNESSNQS DSLLKVPPLR 190 200 210 220 230 240 RTRSATPRAA PVLSRMVEAQ SHFDLTSATP YLPPNNPDKS MEDQPQSSAS ALSMRLMKMF 250 260 270 280 290 300 EFAKPEKVLV EYACSLLQSM LLQGYMYVTE GHICFYAYLP KKSNVAIKSG YLSKRGRKNP 310 320 330 340 350 360 KYHRYWFALK GDVLSYYADP SNLYFPSGHV DLRYGISASL TERKDGDAKD FQVTTDQRTY 370 380 390 400 410 420 LFRADSATSA KEWVKALQQV IFRTHNEGDS VKVSFPIANV IDLEESPMAD FAETFKIRVI 430 440 450 460 470 480 DSGETYAIDE YFFSFFDSGQ DAFNYIKGLV NAVSTTTATK EAQDQHDIKH QPESSRTAYK 490 500 510 520 530 540 RQSVSSVRVS DQRREDSPRK RSSSVGNENQ GSADSFAEQG TGSSPIIQSM TDTTESASQI 550 560 570 580 590 600 LHRSDVFQAP TMRTLQGRSL DVGESIRRYS DDTTPSASAR LDLDAAVGSP CGTLGHNETT 610 620 630 640 650 660 EDARYATGQS DLMQSSRPSS VTQLNELVKA GVYPLQRAAG FAEYLKSRSK QMSTLLATES 670 680 690 700 710 720 MGYIEKVSGM WIGGQKHYGE REGPLLEDQN VDPEDNEGSF NYGDRFRAHF ALPPTEKLQA 730 740 750 760 770 780 TYYAYLHRVL PLYGKIYISQ KKLCFRSLIP GTRTKMILPF KDIENVEKEK GFRFGYHGLV 790 800 810 820 830 840 VIIRGHEELF FEFNASDSRD DCAVTLHQNL ESVKFLVESG LLAEDERDEV EAAKAEHRML 850 860 870 880 890 900 EEARLDSPEG HDAPPTLTED SSEIHPFFDD PRASIINFKP PEPLRITCLT IGSRGDVQPY 910 920 930 940 950 960 IALCKGLLAE GHKPKIATHA EFEPWIRKHG IDFAPVDGDP AELMRICVEN GMFTYSFLRE 970 980 990 1000 1010 1020 ASLKFRGWID DLLSSAWIGC QGSDLLIESP SAMAGIHIAE ALRIPYFRGF TMPWTRTRAY 1030 1040 1050 1060 1070 1080 PHAFAVPENR MGGAYNYITY VMFDNIFWKA IAGQVNRWRN NELGLKATTL DKMQQNKVPF 1090 1100 1110 1120 1130 1140 LYNYSPSVVA PPLDYPDWIR ITGYWFLNEG TDWTPPTELS NFIAQARSDG KKLVYIGFGS 1150 1160 1170 1180 1190 1200 IVVSDPSALT RTVIESVLKA DVRCILSKGW SDRLGDPAST KTEIPLPPEI LQIQSAPHDW 1210 1220 1230 1240 1250 1260 LFSQIDAAAH HGGAGTTGAS LRAGVPTIVK PFFGDQFFFG SRVEDLGVGI CMKKLNVSVF 1270 1280 1290 1300 1310 1320 SRALWEATHS ERMIVKARNL GIQIRNEDGV ATAIQALYRD LEYAKTLARQ KSLASSTPFS 1330 1340 1350 1360 1370 PTPTAKASPD GGDDDLDDIE EWTFVGDETG FDIAKRMRER AASDADRIGS NMFQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ATG genes involved in non-selective autophagy are conserved from yeast to man, but the selective Cvt and pexophagy pathways also require organism-specific genes." Meijer W.H., van der Klei I.J., Veenhuis M., Kiel J.A.K.W. Autophagy 3:106-116(2007) [PubMed: 17204848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]	"Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum." van den Berg M.A., Albang R., Albermann K., Badger J.H., Daran J.-M., Driessen A.J.M., Garcia-Estrada C., Fedorova N.D., Harris D.M., Heijne W.H.M., Joardar V.S., Kiel J.A.K.W., Kovalchuk A., Martin J.F., Nierman W.C., Nijland J.G., Pronk J.T., Roubos J.A. , van der Klei I.J., van Peij N.N.M.E., Veenhuis M., von Doehren H., Wagner C., Wortman J.R., Bovenberg R.A.L. Nat. Biotechnol. 26:1161-1168(2008) [PubMed: 18820685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF110900 Genomic DNA. Translation: ABO31321.1. AM920427 Genomic DNA. Translation: CAP81246.1.
RefSeq	XP_002558417.1.
3D structure databases
SMR	A7KAN4. Positions 283-392, 884-1296.
ModBase	Search...
Genome annotation databases
GeneID	8306628.
GenomeReviews	Gene locus atg26 in contig AM920427_GR.
KEGG	pcs:Pc12g16190.
Phylogenomic databases
OrthoDB	EOG9908S8.
Enzyme and pathway databases
BRENDA	2.4.1.173. 285.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 2 hits. PF00169. PH. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_PENCW

Accession

Primary (citable) accession number: A7KAN4
Secondary accession number(s): B6GYP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 11, 2007
Last modified:	January 19, 2010
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)