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Reviewed, UniProtKB/Swiss-Prot A7KAM5 (ATG15_PENCW)

Last modified October 13, 2009. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Putative lipase atg15
    EC=3.1.1.3
Alternative name(s):
    Autophagy-related protein 15
Gene names
Name: atg15
ORF Names: Pc22g21230
OrganismPenicillium chrysogenum (strain ATCC 28089 / DSM 1075 / Wisconsin 54-1255) (Penicillium notatum) [Complete proteome]
Taxonomic identifier500485 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium › Penicillium chrysogenum complex

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Protein attributes

Sequence length673 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.

Catalytic activity

Triacylglycerol + H2O = diacylglycerol + a carboxylate.

Subcellular location

Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.

Sequence similarities

Belongs to the AB hydrolase superfamily. Lipase family.

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Ontologies

Keywords
   Biological processAutophagy
Lipid degradation
   Cellular componentEndoplasmic reticulum
Endosome
Golgi apparatus
Membrane
   DomainSignal-anchor
Transmembrane
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processautophagy

Inferred from electronic annotation. Source: UniProtKB-KW

lipid catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentGolgi apparatus

Inferred from electronic annotation. Source: UniProtKB-KW

endoplasmic reticulum

Inferred from electronic annotation. Source: UniProtKB-KW

endosome

Inferred from electronic annotation. Source: UniProtKB-KW

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiontriglyceride lipase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 673673Putative lipase atg15
PRO_0000317967

Regions

Topological domain1 – 77Cytoplasmic By similarity
Transmembrane8 – 2821Signal-anchor for type II membrane protein
Topological domain29 – 673645Lumenal By similarity
Compositional bias462 – 672211Thr-rich

Sites

Active site3111Charge relay system By similarity

Amino acid modifications

Glycosylation1561N-linked (GlcNAc...) Potential
Glycosylation1911N-linked (GlcNAc...) Potential
Glycosylation2131N-linked (GlcNAc...) Potential
Glycosylation2711N-linked (GlcNAc...) Potential
Glycosylation2951N-linked (GlcNAc...) Potential
Glycosylation4571N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
A7KAM5-1 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 0804919E2F352AC2

FASTA67372,414
        10         20         30         40         50         60 
MPRKRSRFEL SIHSLLLSVA VLSGAAYASG YYPPSQQVPI IPPQVPLKGA EATPDIHEFS 

        70         80         90        100        110        120 
LRHIFHRGTY EQPDFHARLD VKPDTRLRTV SEDGHEEEYI ASESSLLASS SPLTIQRLAD 

       130        140        150        160        170        180 
RRLPVIQGHL AAARSSGFAA ALSPQEWALD TLPGPNITDK PTVLTFAQMT ANDYIEEPGT 

       190        200        210        220        230        240 
GQWHTINGKF NYSGSFGWQK DGLRGHIYSD KTNGTVVISL KGTSPALFDG AGTTTNDKVN 

       250        260        270        280        290        300 
DNLYFSCCCG QGGSYLWRQS CDCQSATFTA NLTCIIESMT DEDRYYRAAI DLYSNVTEIY 

       310        320        330        340        350        360 
PDANIWMTGH SLGGAMTSLV GLTFGLPVVT FEAVPEALPA ARLGLPSPPG YDPRFPQSRQ 

       370        380        390        400        410        420 
YTGAYHFGHT ADPVFMGTCN GINSICTWGG YAMESVCHTG QVCTYDTVAD KGWRVGLGTH 

       430        440        450        460        470        480 
KIENVISDVL MTYDSVPSCV AEEECFDCEL WKFFRSNGSE ITTTTTTTTT TTSPTRTSTC 

       490        500        510        520        530        540 
KTPGWWGCLD ESTTATTTTT TTTTSTATTT TCMTPGWFGC NDPTTTTATP APTVTTTLPT 

       550        560        570        580        590        600 
VTSTTTSCHD PGWFGCRDET STVATTTANP ASPTSTACHS PGIFWGCWDE PTSTTAVTSL 

       610        620        630        640        650        660 
PAFTSAPIST TCHSPGIFWG CWDEPTSTTA VTSLPAITST PISTTCHIPG IFWGCWDEPT 

       670 
NTPAVTSAPT PTS

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References

« Hide 'large scale' references
[1]"ATG genes involved in non-selective autophagy are conserved from yeast to man, but the selective Cvt and pexophagy pathways also require organism-specific genes."
Meijer W.H., van der Klei I.J., Veenhuis M., Kiel J.A.K.W.
Autophagy 3:106-116(2007) [PubMed: 17204848] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
[2]"Genome sequencing and analysis of the filamentous fungus Penicillium chrysogenum."
van den Berg M.A., Albang R., Albermann K., Badger J.H., Daran J.-M., Driessen A.J.M., Garcia-Estrada C., Fedorova N.D., Harris D.M., Heijne W.H.M., Joardar V.S., Kiel J.A.K.W., Kovalchuk A., Martin J.F., Nierman W.C., Nijland J.G., Pronk J.T., Roubos J.A. expand/collapse author list , van der Klei I.J., van Peij N.N.M.E., Veenhuis M., von Doehren H., Wagner C., Wortman J.R., Bovenberg R.A.L.
Nat. Biotechnol. 26:1161-1168(2008) [PubMed: 18820685] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

EF107747 Genomic DNA. Translation: ABO31085.1.
AM920437 Genomic DNA. Translation: CAP99411.1.
RefSeqXP_002566020.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID8305265.
GenomeReviewsGene locus atg15 in contig AM920437_GR.

Enzyme and pathway databases

BRENDA3.1.1.3. 285.

Family and domain databases

InterProIPR008262. Lipase_Ser_AS.
[Graphical view]
PROSITEPS00120. LIPASE_SER. False negative.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameATG15_PENCW
AccessionPrimary (citable) accession number: A7KAM5
Secondary accession number(s): B6HSF9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: September 11, 2007
Last modified: October 13, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents