Reviewed, UniProtKB/Swiss-Prot A7KAK6 (ATG26_PICAN)

Names and origin

Protein names

Recommended name:
    Sterol 3-beta-glucosyltransferase
    EC=2.4.1.173
Alternative name(s):
    Autophagy-related protein 26

Gene names

Name:

ATG26

Organism

Pichia angusta (Yeast) (Hansenula polymorpha)

Taxonomic identifier

4905 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	1241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May be involved in decane metabolism and autophagy. Involved in the biosynthesis of sterol glucoside By similarity.
Catalytic activity	UDP-glucose + a sterol = UDP + a sterol 3-beta-D-glucoside.
Subcellular location	Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the glycosyltransferase 28 family. Contains 2 GRAM domains. Contains 1 PH domain.

Ontologies

Keywords
Biological process	Autophagy Lipid synthesis Protein transport Steroid biosynthesis Sterol biosynthesis Transport
Cellular component	Cytoplasm Membrane
Domain	Repeat
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW sterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sterol 3-beta-glucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1241	1241	Sterol 3-beta-glucosyltransferase		PRO_0000318047
Regions
	Domain	220 – 267	48	GRAM 1
	Domain	271 – 373	103	PH
	Domain	609 – 675	67	GRAM 2

Sequences

	Sequence		Length	Mass (Da)
	A7KAK6-1 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: DD2EF8120266BE7B	FASTA	1,241	139,706
	10 20 30 40 50 60 MSGIESTEEP CDSMTESQSL SPLEPEERQT RKDSGLQSQS LTKRHFAFSP HRLITSISRH 70 80 90 100 110 120 TSPHKPRPKS ESRVPVPKLH SLPPQRLASP ERDKPSIEGI SKSFVSFLTA ASVYAGFQDL 130 140 150 160 170 180 EGEDQQVSPD DVSAGESEDQ QADESSDEQE DDDQDDRTFY ATDPSPTELT INENTPLPEP 190 200 210 220 230 240 EPPRRTLRKA RSRFEFSVVK RLSENENDQL AQKRAIALSN KLKRTFDISD TDVFISDYPC 250 260 270 280 290 300 WLMGDVLLQG HLYITKHHIL FFAFLPKKQG SISKSGALTT KSYPSLREHR KWAVLRNNTF 310 320 330 340 350 360 SVYSNSTDLY FPLLVIDLRT ALRAEILQSS STKQNPSKPV WIRIITESRT HWFLADNLAS 370 380 390 400 410 420 ARSWVSSLKK HIFASRNKGD QVAIKIPLQN VVDLELTSVI GVTKNLRIKV IESADTFAID 430 440 450 460 470 480 DYFLMFFSRG EKAVEDIKKV IHDAGMEISE GTSTESEDEQ GVDNNLLKSK IELLKKSPSM 490 500 510 520 530 540 VQTSSKSNVP VIRMDEPADD FSQEQESAES SKPVSDDEIV SADDNQELEE KQPQDNLANA 550 560 570 580 590 600 EKENHDKVSR ANSRRTWSTR SLVQGLTAIT QGWMSPSPMA HFDEKYAVLR GEEDSYFVKD 610 620 630 640 650 660 AEQRKAATER FRKHFSLTDG EKLIATYHAY LVKGIPAYGK IYLGSNEMCF RSTLPGTGTI 670 680 690 700 710 720 MILPFSDIEN VNKEKGFRFG YSGLVVVIHG HEELFFEFAS DQARDDCEFM LLKQMDMFKK 730 740 750 760 770 780 GSTDSSPPNA SEGSSDESCN LAESNTSLAS ARLRMFENRI HDAIGLDVPI IIEEHPLTKT 790 800 810 820 830 840 KVRPLKSYRF TLLTIGSRGD VQPYIALGKA LMKEGHQVRI VTHAEFEPWI KKHGIRFASI 850 860 870 880 890 900 AGDPSELMAL MVTHPTINYN FIKEAKSKFR SWIDDLLVTS WKACQDTDIL IESPSSICGI 910 920 930 940 950 960 HIAEKLQIPY FRAFTMPWTR TRAYPHAFMV PDQKLGGAYN YMTHVAFENG YWRGTAHQVN 970 980 990 1000 1010 1020 KWRVETLGLP KTSLAEMKQN NVPFLYNVSP TVFPPSVDFA EWVKVTGYWF LDESETYQPP 1030 1040 1050 1060 1070 1080 EVLTKFIEQA RKDGKKVVYI GFGSIVVSKP SELTQAVVDA VLEADVRCIL NKGWSDRLGT 1090 1100 1110 1120 1130 1140 KTEIEVVLPP EIYNAGSVPH DWLFPQIDAA VHHGGSGTTG ASLRFGVPTI IKPFFGDQKF 1150 1160 1170 1180 1190 1200 YAGRVEDLGC GVSLKDLNYK SLARALKEVT TNTRIIEKAK LVGARIRSET GVQTAIETIY 1210 1220 1230 1240 NEMEYARSLS ISKVKQVSVV KSDEEFDDDK DEEVEGSWLL V

References

[1]

"ATG genes involved in non-selective autophagy are conserved from yeast to man, but the selective Cvt and pexophagy pathways also require organism-specific genes." Meijer W.H., van der Klei I.J., Veenhuis M., Kiel J.A.K.W. Autophagy 3:106-116(2007) [PubMed: 17204848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 34438 / CBS 4732 / DSM 70277 / IFO 1476 / NRRL Y-5445.

Cross-references

Sequence databases
EMBLGenBankDDBJ	EF107728 Genomic DNA. Translation: ABO31066.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.4.1.173. 276392.
Family and domain databases
InterPro	IPR004276. Glyco_trans_28. IPR004182. GRAM. IPR011993. PH_type. IPR001849. Pleckstrin_homology. [Graphical view]
Gene3D	G3DSA:2.30.29.30. PH_type. 1 hit.
Pfam	PF03033. Glyco_transf_28. 1 hit. PF02893. GRAM. 2 hits. PF00169. PH. 1 hit. [Graphical view]
SMART	SM00568. GRAM. 2 hits. SM00233. PH. 1 hit. [Graphical view]
PROSITE	PS50003. PH_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG26_PICAN

Accession

Primary (citable) accession number: A7KAK6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 11, 2007
Last modified:	October 13, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)