A7KAJ9 (ATG15_PICAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative lipase ATG15
EC=3.1.1.3

Alternative name(s):
Autophagy-related protein 15

Gene names

Name:

ATG15

Organism

Pichia angusta (Yeast) (Hansenula polymorpha)

Taxonomic identifier

870730 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetales incertae sedis › Ogataea

Protein attributes

Sequence length	536 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity. Ref.1
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW multivesicular body membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 536	536	Putative lipase ATG15		PRO_0000317969
Regions
	Topological domain	1 – 8	8	Cytoplasmic By similarity
	Transmembrane	9 – 31	23	Helical; Signal-anchor for type II membrane protein
	Topological domain	32 – 536	505	Lumenal By similarity
Sites
	Active site	309	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	152	1	N-linked (GlcNAc...) Potential
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
	Glycosylation	293	1	N-linked (GlcNAc...) Potential
	Glycosylation	426	1	N-linked (GlcNAc...) Potential
	Glycosylation	516	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	A7KAJ9 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 0CDAF454A79D4330	FASTA	536	60,260
	10 20 30 40 50 60 MGNPTLMRWP VTRILVLGLV VTVLYKAMAI YSSRRAPVQV CFTSPSSAFK IKHIFHNAAD 70 80 90 100 110 120 GRTHKRLDID DEFLSIYNSA RSQMAHIEDM DLFTVSPFEQ ELVIRHTEGQ TVRLADRSPD 130 140 150 160 170 180 FVESYLDYAL ENGPASRMIE FDWQQESLLI PNMSDKETIV SLALMSSDAY VGLPTDADWN 190 200 210 220 230 240 DVGDKYNESE RFGWENGGVR GHVFTDPEET IVIISIKGTS AAGLNTGGDG QTVEQDKTND 250 260 270 280 290 300 NLLFSCCCAR VSSLWKTVCD CYEESYTCNQ NCLEQELRRP DRYYKAVLEI YRNVTHMYPE 310 320 330 340 350 360 SEIWVTGHSL GGSLSSLLGR TFGLPAVSFE AVGELLATRR LHLPMPPGLP EEMENIWHVG 370 380 390 400 410 420 NTADPIFMGV CNGASSTCSL AGYALETQCH SGKKCVYDVV NDLGWHVNLL NHRIRTIISD 430 440 450 460 470 480 VLMVYNDTAE CVKPPPCYDC YNWRFVDHSG DRYRTTASSP SPIPSDPPGR RCLKRTWYGR 490 500 510 520 530 CYEWADDPSA VKTTFATSTI YRKTSDISTS THTGGNTTCI RRSWLGYCLE YGPELR

References

[1]

"ATG genes involved in non-selective autophagy are conserved from yeast to man, but the selective Cvt and pexophagy pathways also require organism-specific genes." Meijer W.H., van der Klei I.J., Veenhuis M., Kiel J.A.K.W. Autophagy 3:106-116(2007) [PubMed: 17204848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 34438 / CBS 4732 / DSM 70277 / JCM 3621 / NBRC 1476 / NRRL Y-5445.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EF107721 Genomic DNA. Translation: ABO31059.1.
3D structure databases
ProteinModelPortal	A7KAJ9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002921. Lipase_3. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_PICAN

Accession

Primary (citable) accession number: A7KAJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 11, 2007
Last modified:	December 14, 2011
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program