Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A7J3A4 (VP3_ROTYO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein VP3

Including the following 2 domains:

  1. mRNA guanylyltransferase
    EC=2.7.7.50
  2. mRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.56
OrganismRotavirus A (strain Human/Japan/YO/1977 G3-P1A[8]-I1-R1-C1-M1-Ax-Nx-Tx-E1-Hx) (RV-A)
Taxonomic identifier578832 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length835 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Multifunctional enzyme involved in mRNA capping. Catalyzes the formation of the 5' cap structure on the viral plus-strand transcripts. Specifically binds to GTP and displays guanylyltransferase and methyltransferase activities. Has affinity for ssRNA but not for dsRNA. Capping activity is non-specific and caps RNAs that initiate with either a G or an A residue. Together with VP1 polymerase, forms an enzyme complex positioned near the channels situated at each of the five-fold vertices of the core. Following infection, the outermost layer of the virus is lost, leaving a double-layered particle (DLP) made up of the core and VP6 shell. VP1 then catalyzes the transcription of fully conservative plus-strand genomic RNAs that are capped by VP3 and extruded through the DLP's channels into the cytoplasm where they function as mRNAs for translation of viral proteins. DLPs probably have an RNA triphosphatase activity as well, whereas open cores don't By similarity.

Catalytic activity

GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.

S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.

Subunit structure

Interacts with VP1 Potential. Interacts with VP2 By similarity.

Subcellular location

Virion Potential. Note: Attached inside the inner capsid as a minor component. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging Potential.

Sequence similarities

Belongs to the rotavirus VP3 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 835835Protein VP3
PRO_0000368081

Regions

Compositional bias270 – 2734Poly-Tyr

Sequences

Sequence LengthMass (Da)Tools
A7J3A4 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 71764A948584E364

FASTA83598,021
        10         20         30         40         50         60 
MKVLALRHSV AQVYADTQTY IHDDSKDEYE NAFLISNLTT HNILYLNYSF KTLKILNKSG 

        70         80         90        100        110        120 
IAAIEVQSPD ELFALIRCNF TYDYENNIVY LHDYSYYTNN EIRTDQYWIT KTDIINYLLP 

       130        140        150        160        170        180 
GWKLTHVGYN GKNTRGHYNF SFICQNAATD DDIIIEYIYS NELDFQNFLL RKIKERMTTS 

       190        200        210        220        230        240 
LPIARLSNRV FRDKLFPSIV NIHKKVINVG PRNESMFTFL NFPTIKQFSN GAYIVKHTIK 

       250        260        270        280        290        300 
LKQEKWLGKR ISQFDIGQYK NMLNVVTTIY YYYNLYYSKP VIYMLGSAPS YWIYDIKQYS 

       310        320        330        340        350        360 
DFTFETWDPL DTPYSTIHHK ELFFDKDINK LKDNSVLYID IRTDRGNMDW KEWRKIVEQQ 

       370        380        390        400        410        420 
TVNNLNIAYK YLSTGKAKVC CVKLTAMDLE LPITAKLLHH PTTEIRSEFY AILDVWDIIT 

       430        440        450        460        470        480 
IKRFIPKGVF YAFINNVTTE NVFIQPPFKL KMSPTDYIVA LYALSNDFNS RQDIINLINK 

       490        500        510        520        530        540 
QKQSLITVRI NNTFKDEPKV SFKNIYDWTF LPTDFELKDS VITSYDGCLG MFGLSISLSP 

       550        560        570        580        590        600 
KPTGNNHLFI INGTDKYYKL DQYANHTGIS RRSHQIRFSE SATSYSGYIF RDLSNNNFNL 

       610        620        630        640        650        660 
IGTNVENSVS GHVYNALIYY RYNYAFDLKR WIYLHSIGKV AVEGGRYYEH APIELIYACR 

       670        680        690        700        710        720 
SAKEFAILQD DLTVLRYANE IERYINKVYS ITYADDPNYF IGIKFNSIPY EYDVKVPHLT 

       730        740        750        760        770        780 
LGVLFISDNM VHNVITVLKK MKTELFKMEI STSYTYMLSD NTYVANVSGV LSTYFKLYNM 

       790        800        810        820        830 
FYRNHVTFGQ SRMFIPHITL SFSNKQTVRI ESTKLRINSI YLRKIKGETV FDMSE 

« Hide

References

[1]"Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains."
Matthijnssens J., Ciarlet M., Heiman E.M., Arijs I., Delbeke T., McDonald S.M., Palombo E.A., Iturriza-Gomara M., Maes P., Patton J.T., Rahman M., Van Ranst M.
J. Virol. 82:3204-3219(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ870499 Genomic RNA. Translation: ABI60854.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR011181. VP3_Rotav.
[Graphical view]
PfamPF06929. Rotavirus_VP3. 1 hit.
[Graphical view]
PIRSFPIRSF004015. LigT_rotavirus. 1 hit.
PROSITEPS51589. SAM_MT56_VP3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameVP3_ROTYO
AccessionPrimary (citable) accession number: A7J3A4
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 11, 2007
Last modified: April 16, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families