Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A7J391 (VP2_ROTHS) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inner capsid protein VP2
OrganismRotavirus A (strain Human/Japan/S2/1980 G2-P1B[4]-I2-R2-C2-M2-Ax-Nx-T2-E2-Hx) (RV-A)
Taxonomic identifier10959 [NCBI]
Taxonomic lineageVirusesdsRNA virusesReoviridaeSedoreovirinaeRotavirusRotavirus A
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length879 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Inner capsid protein that self assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nacent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels at the five-fold axes. VP2 is required for the replicase activity of VP1 polymerase. It probably plays a role in the coordination of packaging and genome replication by controlling the initiation of minus-strand synthesis. Binding to the polymerase VP1 presumably activates the autoinhibited VP1-RNA complex which will start the synthesis of the complementary minus-strand By similarity.

Subunit structure

Dimer Potential. Interacts with VP1; this interaction presumably activates VP1. Interacts with VP3. Interacts with VP6. Interacts with NSP5 By similarity.

Subcellular location

Virion Potential. Note: Inner capsid protein. Also found in spherical cytoplasmic structures, called virus factories, that appear early after infection and are the site of viral replication and packaging.

Domain

The N-terminus is involved in VP1 binding By similarity.

Sequence similarities

Belongs to the rotavirus VP2 family.

Ontologies

Keywords
   Cellular componentCapsid protein
Inner capsid protein
Virion
   DomainRepeat
   LigandRNA-binding
Gene Ontology (GO)
   Cellular_componentviral inner capsid

Inferred from electronic annotation. Source: UniProtKB-KW

viral nucleocapsid

Inferred from electronic annotation. Source: InterPro

   Molecular_functionRNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 879879Inner capsid protein VP2
PRO_0000368058

Regions

Region1 – 9191Interaction with VP1 and VP3 By similarity
Region393 – 41321Hydrophobic By similarity
Region421 – 44121Hydrophobic By similarity
Region535 – 55622Leucine-zipper 1 Potential
Region664 – 68522Leucine-zipper 2 Potential

Sequences

Sequence LengthMass (Da)Tools
A7J391 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: D2BA3E351480F151

FASTA879102,398
        10         20         30         40         50         60 
MAYRKRGARR EANLNNNDRM QEKNDEKQDS NKIQLSDKVL SKKEEVVTDS HEEVKITDEV 

        70         80         90        100        110        120 
KKSTKEESKQ LLEVLKTKEE HQKEIQYEIL QKTIPTFEPK ETILRKLEDI KPELAKKQTK 

       130        140        150        160        170        180 
LFRIFEPKQL PIYRANGERE LRNRWYWKLK KDTLPDGDYD VREYFLNLYD QVLTEMPDYL 

       190        200        210        220        230        240 
LLKDMAVENK NSRDAGKVVD SETASICDAI FQDEETEGAV RRFIAEMRQR VQADRNVVNY 

       250        260        270        280        290        300 
PSILHPIDYA FNEYFLQHQL VEPLNNDIIF NYIPERIRND VNYILNMDRN LPSTARYIRP 

       310        320        330        340        350        360 
NLLQDRLNLH DNFESLWDTI TTSNYILARS VVPDLKELVS TEAQIQKMSQ DLQLEALTIQ 

       370        380        390        400        410        420 
SETQFLTGIN SQAANDCFKT LIAAMLSQRT MSLDFVTTNY MSLISGMWLL TVVPNDMFIR 

       430        440        450        460        470        480 
ESLVACQLAI VNTIIYPAFG MQRMHYRNGD PQTPFQIAEQ QIQNFQVANW LHFVNNNQFR 

       490        500        510        520        530        540 
QAVIDGVLNQ VLNDNIRNGH VINQLMEALM QLSRQQFPTM PVDYKRSIQR GILLLSNRLG 

       550        560        570        580        590        600 
QLVDLTRLLA YNYETLMACI TMNMQHVQTL TTEKLQLTSV TSLCMLIGNA TVIPSPQTLF 

       610        620        630        640        650        660 
HYYNVNVNFH SNYNERINDA VAIITAANRL NLYQKKMKAI VEDFLKRLYI FDVSRVPDDQ 

       670        680        690        700        710        720 
MYRLRDRLRL LPVEIRRLDI FNLILMNMDQ IERASDKIAQ GVIIAYRDMH LERDEMYGYV 

       730        740        750        760        770        780 
NIARNLEGFQ QINLEELMRS GDYAQITNML LNNQPVALVG ALPFITDSSV ISLIAKLDAT 

       790        800        810        820        830        840 
VFAQIVKLRK VDTLKPILYK INSDSNDFYL VANYDWVPTS TTKVYKQVPQ QFDFRNSMHM 

       850        860        870 
LTSNLTFTVY SDLLAFVSAD TVEPINAVAF DNMRIMNEL 

« Hide

References

[1]"Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-like and bovine rotavirus strains."
Matthijnssens J., Ciarlet M., Heiman E.M., Arijs I., Delbeke T., McDonald S.M., Palombo E.A., Iturriza-Gomara M., Maes P., Patton J.T., Rahman M., Van Ranst M.
J. Virol. 82:3204-3219(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Molecular epidemiology of G9 rotaviruses in Taiwan between 2000 and 2002."
Lin Y.P., Chang S.Y., Kao C.L., Huang L.M., Chung M.Y., Yang J.Y., Chen H.Y., Taniguchi K., Tsai K.S., Lee C.N.
J. Clin. Microbiol. 44:3686-3694(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 148-316.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
DQ870486 Genomic RNA. Translation: ABI60845.1.
DQ490203 Genomic RNA. Translation: ABF57055.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR007779. Rotavirus_VP2.
[Graphical view]
PfamPF05087. Rota_VP2. 1 hit.
[Graphical view]
ProDomPD008866. Rota_VP2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Entry information

Entry nameVP2_ROTHS
AccessionPrimary (citable) accession number: A7J391
Secondary accession number(s): Q0Q7L8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: September 11, 2007
Last modified: October 16, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families