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A7IPJ5 (SYE2_XANP2) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamate--tRNA ligase 2

EC=6.1.1.17
Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2
Gene names
Name:gltX2
Ordered Locus Names:Xaut_4722
OrganismXanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) [Complete proteome] [HAMAP]
Taxonomic identifier78245 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter

Protein attributes

Sequence length458 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP-Rule MF_00022

Catalytic activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP-Rule MF_00022

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00022

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00022.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamate-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 458458Glutamate--tRNA ligase 2 HAMAP-Rule MF_00022
PRO_0000367796

Regions

Motif20 – 3011"HIGH" region HAMAP-Rule MF_00022
Motif251 – 2555"KMSKS" region HAMAP-Rule MF_00022

Sites

Binding site2541ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A7IPJ5 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: 3919D25CB63F2CF0

FASTA45848,931
        10         20         30         40         50         60 
MSRSTPSRST PSRPVLRFAP SPTGLIHVGN ARTALINALL ARRAGGTFIL RFDDTDAARS 

        70         80         90        100        110        120 
RAEYADAIAT DLAWLGIPPD LTFRQSDRIG EYEAATQQLK ASGRLYPAYE SEDELELKRR 

       130        140        150        160        170        180 
LQRARSLPPV YDRAALALTA ADRARLEAEG RRPHWRFRLD HRVVAWDDGV RGPQQVDTAS 

       190        200        210        220        230        240 
LSDPVLVRAD GSFLYTLPSV VDDLAMGVTD VVRGEDHVTN TAVQIEIFEA LGGVAPRFAH 

       250        260        270        280        290        300 
HNLLTLPSGE GLSKRLGHLS LSALREAGQE ALAVAAAAVL VGTSHAVEAV ESLEALAGMV 

       310        320        330        340        350        360 
DLAHISRAPA RFDPDDLAQL TARTLHMMPF EAAASRLAAA GIGGGVAFWL AVRGNLARFS 

       370        380        390        400        410        420 
EAADWWALVS QPAAGVVAES DRTFLGEAAA LLPPEPWDGQ TYANWIKAVK AASGQSGKAL 

       430        440        450 
FHPLRLALTG LEKGPELAAL LPLMGRERVA GRLGGGVA 

« Hide

References

[1]"Complete sequence of chromosome of Xanthobacter autotrophicus Py2."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Brainard J., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Ensigns S.A., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1158 / Py2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000781 Genomic DNA. Translation: ABS69941.1.
RefSeqYP_001419598.1. NC_009720.1.

3D structure databases

ProteinModelPortalA7IPJ5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING78245.Xaut_4722.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS69941; ABS69941; Xaut_4722.
GeneID5421482.
KEGGxau:Xaut_4722.
PATRIC24051662. VBIXanAut29526_5084.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000252721.
KOK01885.
OMAFFFANAV.
OrthoDBEOG6DRPF7.
ProtClustDBCLSK2793726.

Enzyme and pathway databases

BioCycXAUT78245:GHS6-4781-MONOMER.

Family and domain databases

Gene3D1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPMF_00022_B. Glu_tRNA_synth_B.
InterProIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF48163. SSF48163. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYE2_XANP2
AccessionPrimary (citable) accession number: A7IPJ5
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: September 11, 2007
Last modified: February 19, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries