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A7INE1 (TRMFO_XANP2) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene names
Name:trmFO
Ordered Locus Names:Xaut_4314
OrganismXanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) [Complete proteome] [HAMAP]
Taxonomic identifier78245 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP-Rule MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + uracil54 in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil54 in tRNA + FAD. HAMAP-Rule MF_01037

Cofactor

FAD By similarity. HAMAP-Rule MF_01037

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01037.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 471471Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP-Rule MF_01037
PRO_0000346421

Regions

Nucleotide binding12 – 176FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
A7INE1 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: A24CF40C4C5BD638

FASTA47150,555
        10         20         30         40         50         60 
MPQSADPVHV VGGGLAGSEA AWQIATRGVP VVLHEMRPQR TTDAHLTDGL AELVCSNSFR 

        70         80         90        100        110        120 
SDDPEGNAVG VLHAEMRQAG SLILTAADQN QVPAGGALAV DRDGFSRAVT KALEDHPLIE 

       130        140        150        160        170        180 
IRREELTTLP PAEWDKVILA TGPLTSPALA EAVLSLTGET ALAFFDAIAP IVHFDSIDMG 

       190        200        210        220        230        240 
KAWFQSRYDK PGPGGTGADY INCPLDEAQY HAFVDALIAG EKAPMRDFEA KTPYFDGCLP 

       250        260        270        280        290        300 
IEVMAERGRE TLRFGPMKPV GLTNPHNPTV KAYAIVQLRQ DNKLGTLYNM VGFQTKLRHG 

       310        320        330        340        350        360 
EQARVFRTIP GLENAEFARL GGLHRNTYLN SPRLLDGTLR LKADPRLRFA GQITGCEGYV 

       370        380        390        400        410        420 
ESAAVGLMAG RFAAAERRGE ALVPPPLTTA HGALLAHITG GHVEAEEAGA PRSFQPMNIN 

       430        440        450        460        470 
FGLFPPLEEA PRGEPGKRLR GAEKTLAKKK AMAARALAHM AAWLDTPARA A 

« Hide

References

[1]"Complete sequence of chromosome of Xanthobacter autotrophicus Py2."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Brainard J., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Ensigns S.A., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1158 / Py2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000781 Genomic DNA. Translation: ABS69535.1.
RefSeqYP_001419192.1. NC_009720.1.

3D structure databases

ProteinModelPortalA7INE1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING78245.Xaut_4314.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS69535; ABS69535; Xaut_4314.
GeneID5422457.
KEGGxau:Xaut_4314.
PATRIC24050814. VBIXanAut29526_4665.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1206.
HOGENOMHOG000252054.
KOK04094.
OMAEIYFEGC.
OrthoDBEOG6J74VT.

Enzyme and pathway databases

BioCycXAUT78245:GHS6-4368-MONOMER.

Family and domain databases

HAMAPMF_01037. TrmFO.
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00137. gid_trmFO. 1 hit.
PROSITEPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMFO_XANP2
AccessionPrimary (citable) accession number: A7INE1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 11, 2007
Last modified: May 14, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families