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Protein

Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO

Gene

trmFO

Organism
Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + uracil(54) in tRNA + FADH2 = tetrahydrofolate + 5-methyluracil(54) in tRNA + FAD.UniRule annotation

Cofactori

FADUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176FADUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BioCyciXAUT78245:GHS6-4368-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOUniRule annotation (EC:2.1.1.74UniRule annotation)
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferaseUniRule annotation
Folate-dependent tRNA(M-5-U54)-methyltransferaseUniRule annotation
Gene namesi
Name:trmFOUniRule annotation
Ordered Locus Names:Xaut_4314
OrganismiXanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
Taxonomic identifieri78245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter
ProteomesiUP000002417 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 471471Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFOPRO_0000346421Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi78245.Xaut_4314.

Structurei

3D structure databases

ProteinModelPortaliA7INE1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MnmG family. TrmFO subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG1206.
HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.
OrthoDBiEOG6J74VT.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7INE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQSADPVHV VGGGLAGSEA AWQIATRGVP VVLHEMRPQR TTDAHLTDGL
60 70 80 90 100
AELVCSNSFR SDDPEGNAVG VLHAEMRQAG SLILTAADQN QVPAGGALAV
110 120 130 140 150
DRDGFSRAVT KALEDHPLIE IRREELTTLP PAEWDKVILA TGPLTSPALA
160 170 180 190 200
EAVLSLTGET ALAFFDAIAP IVHFDSIDMG KAWFQSRYDK PGPGGTGADY
210 220 230 240 250
INCPLDEAQY HAFVDALIAG EKAPMRDFEA KTPYFDGCLP IEVMAERGRE
260 270 280 290 300
TLRFGPMKPV GLTNPHNPTV KAYAIVQLRQ DNKLGTLYNM VGFQTKLRHG
310 320 330 340 350
EQARVFRTIP GLENAEFARL GGLHRNTYLN SPRLLDGTLR LKADPRLRFA
360 370 380 390 400
GQITGCEGYV ESAAVGLMAG RFAAAERRGE ALVPPPLTTA HGALLAHITG
410 420 430 440 450
GHVEAEEAGA PRSFQPMNIN FGLFPPLEEA PRGEPGKRLR GAEKTLAKKK
460 470
AMAARALAHM AAWLDTPARA A
Length:471
Mass (Da):50,555
Last modified:September 11, 2007 - v1
Checksum:iA24CF40C4C5BD638
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000781 Genomic DNA. Translation: ABS69535.1.
RefSeqiWP_012116283.1. NC_009720.1.
YP_001419192.1. NC_009720.1.

Genome annotation databases

EnsemblBacteriaiABS69535; ABS69535; Xaut_4314.
KEGGixau:Xaut_4314.
PATRICi24050814. VBIXanAut29526_4665.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000781 Genomic DNA. Translation: ABS69535.1.
RefSeqiWP_012116283.1. NC_009720.1.
YP_001419192.1. NC_009720.1.

3D structure databases

ProteinModelPortaliA7INE1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi78245.Xaut_4314.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS69535; ABS69535; Xaut_4314.
KEGGixau:Xaut_4314.
PATRICi24050814. VBIXanAut29526_4665.

Phylogenomic databases

eggNOGiCOG1206.
HOGENOMiHOG000252054.
KOiK04094.
OMAiRFGPMKP.
OrthoDBiEOG6J74VT.

Enzyme and pathway databases

BioCyciXAUT78245:GHS6-4368-MONOMER.

Family and domain databases

HAMAPiMF_01037. TrmFO.
InterProiIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
PfamiPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00137. gid_trmFO. 1 hit.
PROSITEiPS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1158 / Py2.

Entry informationi

Entry nameiTRMFO_XANP2
AccessioniPrimary (citable) accession number: A7INE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: September 11, 2007
Last modified: May 27, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.