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A7IHF8

- A7IHF8_XANP2

UniProt

A7IHF8 - A7IHF8_XANP2

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Xaut_2207
Organism
Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei639 – 6391Aminoacyl-adenylate By similarityUniRule annotation
Binding sitei683 – 6831ATP By similarityUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationsImported, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotations

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotations, Nucleotide-binding

Enzyme and pathway databases

BioCyciXAUT78245:GHS6-2230-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetase
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Xaut_2207Imported
OrganismiXanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)Imported
Taxonomic identifieri78245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter
ProteomesiUP000002417: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi78245.Xaut_2207.

Structurei

3D structure databases

ProteinModelPortaliA7IHF8.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi76 – 8611"HIGH" region By similarityUniRule annotation
Add
BLAST
Motifi680 – 6845"KMSKS" region By similarityUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7IHF8-1 [UniParc]FASTAAdd to Basket

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MTAPDSSAPD TATAEASAAP DYSKTLLLPQ TAFPMRAGLP KLEPELLARW     50
ARTDLYGRLR EQGRGRARFV LHDGPPYANG NIHIGHALNK ILKDVVTRSQ 100
QMLGYDSNYV PGWDCHGLPI EWKIEEDNYR KKGKTKPDFT DAAAMVAFRQ 150
ECRAYAEHWL SEQRKEFKRL GVEGDWDHPY TTMAFAAEAQ IAREIMKFAE 200
NGLLYRGSKP VMWSVVEKTA LAEAEVEYQD FTSDTVWVKF PVASEGELKG 250
ATVVIWTTTP WTLPGNRAIS YSSKISYGLY EVTEAPADNW AKAGDKLILA 300
DKLAEDVFKQ ARVTTFARRG DVPADVLGRL ITAHPLAALG YSFQVPLLDG 350
DHVTDDAGTG FVHTAPGHGR EDFEIWTQHR RWLEERGISP AIPYTVDADS 400
FYTAQAPGFE GKRVITEKGE KGDANTAVID ALIKAGNLLA RGRVKHQYPH 450
SWRSKKPVIF RNTPQWFIAM DQDIRTADGT AAPRPATLAG NAPDTLRERA 500
LAGIRTVEWV PAAGENRITG MIANRPDWVV SRQRAWGVPI AVFVKENAYG 550
EVVILKDPQV NARIAEAFAA EGADAWFKEG AKERFLSGLV EKPDHWEMVR 600
DVLDVWFDSG STHAFTLEVR PDLKAARPPE GNDRVMYLEG SDQHRGWFHS 650
SLLESCGTRG RPPYDVVLTH GFVLDEDGRK MSKSLGNVTS PQDVIKQSGA 700
DILRLWVCAS DYADDLRIGP EILKTTADTY RKLRNTIRWL LGSLHHDRPA 750
EHVAFADMPA LERYILHRLV ELDGEIRAAY RAFDYKKVNA ALTQFMNIEL 800
SAFYFDIRKD ALYCDPLSSV TRRACLTVLD AVFARLITWL APILPFTCEE 850
AYIARTGDEE GSVHLLGFPD TPADWRDDAL AEKWRQVRVI RRVVLGALEV 900
ERAAKRMGSS LEAAPEVYVS ESALADALDG LDLAEITITS AATLIRSEGP 950
AEAFRLPDVP GVAVVPVRAE GRKCARSWKI SAEVGTDPQF PDVTPRDADA 1000
LREFFAAQTA AQ 1012
Length:1,012
Mass (Da):112,627
Last modified:September 11, 2007 - v1
Checksum:iF751C59A78001778
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000781 Genomic DNA. Translation: ABS67451.1.
RefSeqiYP_001417108.1. NC_009720.1.

Genome annotation databases

EnsemblBacteriaiABS67451; ABS67451; Xaut_2207.
GeneIDi5421678.
KEGGixau:Xaut_2207.
PATRICi24046503. VBIXanAut29526_2541.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000781 Genomic DNA. Translation: ABS67451.1 .
RefSeqi YP_001417108.1. NC_009720.1.

3D structure databases

ProteinModelPortali A7IHF8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 78245.Xaut_2207.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS67451 ; ABS67451 ; Xaut_2207 .
GeneIDi 5421678.
KEGGi xau:Xaut_2207.
PATRICi 24046503. VBIXanAut29526_2541.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci XAUT78245:GHS6-2230-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1158 / Py2.

Entry informationi

Entry nameiA7IHF8_XANP2
AccessioniPrimary (citable) accession number: A7IHF8
Entry historyi
Integrated into UniProtKB/TrEMBL: September 11, 2007
Last sequence update: September 11, 2007
Last modified: June 11, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome

External Data

Dasty 3

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