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A7IHF8

- A7IHF8_XANP2

UniProt

A7IHF8 - A7IHF8_XANP2

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei639 – 6391Aminoacyl-adenylateUniRule annotation
Binding sitei683 – 6831ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotation, Ligase

Keywords - Biological processi

Protein biosynthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciXAUT78245:GHS6-2230-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Xaut_2207Imported
OrganismiXanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)Imported
Taxonomic identifieri78245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter
ProteomesiUP000002417: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi78245.Xaut_2207.

Structurei

3D structure databases

ProteinModelPortaliA7IHF8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi76 – 8611"HIGH" regionUniRule annotationAdd
BLAST
Motifi680 – 6845"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7IHF8 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTAPDSSAPD TATAEASAAP DYSKTLLLPQ TAFPMRAGLP KLEPELLARW
60 70 80 90 100
ARTDLYGRLR EQGRGRARFV LHDGPPYANG NIHIGHALNK ILKDVVTRSQ
110 120 130 140 150
QMLGYDSNYV PGWDCHGLPI EWKIEEDNYR KKGKTKPDFT DAAAMVAFRQ
160 170 180 190 200
ECRAYAEHWL SEQRKEFKRL GVEGDWDHPY TTMAFAAEAQ IAREIMKFAE
210 220 230 240 250
NGLLYRGSKP VMWSVVEKTA LAEAEVEYQD FTSDTVWVKF PVASEGELKG
260 270 280 290 300
ATVVIWTTTP WTLPGNRAIS YSSKISYGLY EVTEAPADNW AKAGDKLILA
310 320 330 340 350
DKLAEDVFKQ ARVTTFARRG DVPADVLGRL ITAHPLAALG YSFQVPLLDG
360 370 380 390 400
DHVTDDAGTG FVHTAPGHGR EDFEIWTQHR RWLEERGISP AIPYTVDADS
410 420 430 440 450
FYTAQAPGFE GKRVITEKGE KGDANTAVID ALIKAGNLLA RGRVKHQYPH
460 470 480 490 500
SWRSKKPVIF RNTPQWFIAM DQDIRTADGT AAPRPATLAG NAPDTLRERA
510 520 530 540 550
LAGIRTVEWV PAAGENRITG MIANRPDWVV SRQRAWGVPI AVFVKENAYG
560 570 580 590 600
EVVILKDPQV NARIAEAFAA EGADAWFKEG AKERFLSGLV EKPDHWEMVR
610 620 630 640 650
DVLDVWFDSG STHAFTLEVR PDLKAARPPE GNDRVMYLEG SDQHRGWFHS
660 670 680 690 700
SLLESCGTRG RPPYDVVLTH GFVLDEDGRK MSKSLGNVTS PQDVIKQSGA
710 720 730 740 750
DILRLWVCAS DYADDLRIGP EILKTTADTY RKLRNTIRWL LGSLHHDRPA
760 770 780 790 800
EHVAFADMPA LERYILHRLV ELDGEIRAAY RAFDYKKVNA ALTQFMNIEL
810 820 830 840 850
SAFYFDIRKD ALYCDPLSSV TRRACLTVLD AVFARLITWL APILPFTCEE
860 870 880 890 900
AYIARTGDEE GSVHLLGFPD TPADWRDDAL AEKWRQVRVI RRVVLGALEV
910 920 930 940 950
ERAAKRMGSS LEAAPEVYVS ESALADALDG LDLAEITITS AATLIRSEGP
960 970 980 990 1000
AEAFRLPDVP GVAVVPVRAE GRKCARSWKI SAEVGTDPQF PDVTPRDADA
1010
LREFFAAQTA AQ
Length:1,012
Mass (Da):112,627
Last modified:September 11, 2007 - v1
Checksum:iF751C59A78001778
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000781 Genomic DNA. Translation: ABS67451.1.
RefSeqiWP_012114235.1. NC_009720.1.
YP_001417108.1. NC_009720.1.

Genome annotation databases

EnsemblBacteriaiABS67451; ABS67451; Xaut_2207.
GeneIDi5421678.
KEGGixau:Xaut_2207.
PATRICi24046503. VBIXanAut29526_2541.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000781 Genomic DNA. Translation: ABS67451.1 .
RefSeqi WP_012114235.1. NC_009720.1.
YP_001417108.1. NC_009720.1.

3D structure databases

ProteinModelPortali A7IHF8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 78245.Xaut_2207.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS67451 ; ABS67451 ; Xaut_2207 .
GeneIDi 5421678.
KEGGi xau:Xaut_2207.
PATRICi 24046503. VBIXanAut29526_2541.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci XAUT78245:GHS6-2230-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1158 / Py2Imported.

Entry informationi

Entry nameiA7IHF8_XANP2
AccessioniPrimary (citable) accession number: A7IHF8
Entry historyi
Integrated into UniProtKB/TrEMBL: September 11, 2007
Last sequence update: September 11, 2007
Last modified: October 29, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3