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A7IHF8 (A7IHF8_XANP2) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase HAMAP-Rule MF_02002

EC=6.1.1.5 HAMAP-Rule MF_02002
Alternative name(s):
Isoleucyl-tRNA synthetase HAMAP-Rule MF_02002
Gene names
Name:ileS HAMAP-Rule MF_02002
Ordered Locus Names:Xaut_2207 EMBL ABS67451.1
OrganismXanthobacter autotrophicus (strain ATCC BAA-1158 / Py2) [Complete proteome] [HAMAP] EMBL ABS67451.1
Taxonomic identifier78245 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter

Protein attributes

Sequence length1012 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002 SAAS SAAS023585

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily. HAMAP-Rule MF_02002

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Motif76 – 8611"HIGH" region By similarity HAMAP-Rule MF_02002
Motif680 – 6845"KMSKS" region By similarity HAMAP-Rule MF_02002

Sites

Binding site6391Aminoacyl-adenylate By similarity HAMAP-Rule MF_02002
Binding site6831ATP By similarity HAMAP-Rule MF_02002

Sequences

Sequence LengthMass (Da)Tools
A7IHF8 [UniParc].

Last modified September 11, 2007. Version 1.
Checksum: F751C59A78001778

FASTA1,012112,627
        10         20         30         40         50         60 
MTAPDSSAPD TATAEASAAP DYSKTLLLPQ TAFPMRAGLP KLEPELLARW ARTDLYGRLR 

        70         80         90        100        110        120 
EQGRGRARFV LHDGPPYANG NIHIGHALNK ILKDVVTRSQ QMLGYDSNYV PGWDCHGLPI 

       130        140        150        160        170        180 
EWKIEEDNYR KKGKTKPDFT DAAAMVAFRQ ECRAYAEHWL SEQRKEFKRL GVEGDWDHPY 

       190        200        210        220        230        240 
TTMAFAAEAQ IAREIMKFAE NGLLYRGSKP VMWSVVEKTA LAEAEVEYQD FTSDTVWVKF 

       250        260        270        280        290        300 
PVASEGELKG ATVVIWTTTP WTLPGNRAIS YSSKISYGLY EVTEAPADNW AKAGDKLILA 

       310        320        330        340        350        360 
DKLAEDVFKQ ARVTTFARRG DVPADVLGRL ITAHPLAALG YSFQVPLLDG DHVTDDAGTG 

       370        380        390        400        410        420 
FVHTAPGHGR EDFEIWTQHR RWLEERGISP AIPYTVDADS FYTAQAPGFE GKRVITEKGE 

       430        440        450        460        470        480 
KGDANTAVID ALIKAGNLLA RGRVKHQYPH SWRSKKPVIF RNTPQWFIAM DQDIRTADGT 

       490        500        510        520        530        540 
AAPRPATLAG NAPDTLRERA LAGIRTVEWV PAAGENRITG MIANRPDWVV SRQRAWGVPI 

       550        560        570        580        590        600 
AVFVKENAYG EVVILKDPQV NARIAEAFAA EGADAWFKEG AKERFLSGLV EKPDHWEMVR 

       610        620        630        640        650        660 
DVLDVWFDSG STHAFTLEVR PDLKAARPPE GNDRVMYLEG SDQHRGWFHS SLLESCGTRG 

       670        680        690        700        710        720 
RPPYDVVLTH GFVLDEDGRK MSKSLGNVTS PQDVIKQSGA DILRLWVCAS DYADDLRIGP 

       730        740        750        760        770        780 
EILKTTADTY RKLRNTIRWL LGSLHHDRPA EHVAFADMPA LERYILHRLV ELDGEIRAAY 

       790        800        810        820        830        840 
RAFDYKKVNA ALTQFMNIEL SAFYFDIRKD ALYCDPLSSV TRRACLTVLD AVFARLITWL 

       850        860        870        880        890        900 
APILPFTCEE AYIARTGDEE GSVHLLGFPD TPADWRDDAL AEKWRQVRVI RRVVLGALEV 

       910        920        930        940        950        960 
ERAAKRMGSS LEAAPEVYVS ESALADALDG LDLAEITITS AATLIRSEGP AEAFRLPDVP 

       970        980        990       1000       1010 
GVAVVPVRAE GRKCARSWKI SAEVGTDPQF PDVTPRDADA LREFFAAQTA AQ 

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References

[1]"Complete sequence of chromosome of Xanthobacter autotrophicus Py2."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Sims D., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Brainard J., Schmutz J. expand/collapse author list , Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Ensigns S.A., Richardson P.
Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-1158 / Py2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000781 Genomic DNA. Translation: ABS67451.1.
RefSeqYP_001417108.1. NC_009720.1.

3D structure databases

ProteinModelPortalA7IHF8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING78245.Xaut_2207.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABS67451; ABS67451; Xaut_2207.
GeneID5421678.
KEGGxau:Xaut_2207.
PATRIC24046503. VBIXanAut29526_2541.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAKPVHWCL.
OrthoDBEOG644ZM1.

Enzyme and pathway databases

BioCycXAUT78245:GHS6-2230-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA7IHF8_XANP2
AccessionPrimary (citable) accession number: A7IHF8
Entry history
Integrated into UniProtKB/TrEMBL: September 11, 2007
Last sequence update: September 11, 2007
Last modified: June 11, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)