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A7IHF8

- A7IHF8_XANP2

UniProt

A7IHF8 - A7IHF8_XANP2

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei639 – 6391Aminoacyl-adenylateUniRule annotation
    Binding sitei683 – 6831ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase

    Keywords - Biological processi

    Protein biosynthesisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciXAUT78245:GHS6-2230-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Xaut_2207Imported
    OrganismiXanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)Imported
    Taxonomic identifieri78245 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter
    ProteomesiUP000002417: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    CytoplasmUniRule annotation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi78245.Xaut_2207.

    Structurei

    3D structure databases

    ProteinModelPortaliA7IHF8.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi76 – 8611"HIGH" regionUniRule annotationAdd
    BLAST
    Motifi680 – 6845"KMSKS" regionUniRule annotation

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7IHF8-1 [UniParc]FASTAAdd to Basket

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    MTAPDSSAPD TATAEASAAP DYSKTLLLPQ TAFPMRAGLP KLEPELLARW     50
    ARTDLYGRLR EQGRGRARFV LHDGPPYANG NIHIGHALNK ILKDVVTRSQ 100
    QMLGYDSNYV PGWDCHGLPI EWKIEEDNYR KKGKTKPDFT DAAAMVAFRQ 150
    ECRAYAEHWL SEQRKEFKRL GVEGDWDHPY TTMAFAAEAQ IAREIMKFAE 200
    NGLLYRGSKP VMWSVVEKTA LAEAEVEYQD FTSDTVWVKF PVASEGELKG 250
    ATVVIWTTTP WTLPGNRAIS YSSKISYGLY EVTEAPADNW AKAGDKLILA 300
    DKLAEDVFKQ ARVTTFARRG DVPADVLGRL ITAHPLAALG YSFQVPLLDG 350
    DHVTDDAGTG FVHTAPGHGR EDFEIWTQHR RWLEERGISP AIPYTVDADS 400
    FYTAQAPGFE GKRVITEKGE KGDANTAVID ALIKAGNLLA RGRVKHQYPH 450
    SWRSKKPVIF RNTPQWFIAM DQDIRTADGT AAPRPATLAG NAPDTLRERA 500
    LAGIRTVEWV PAAGENRITG MIANRPDWVV SRQRAWGVPI AVFVKENAYG 550
    EVVILKDPQV NARIAEAFAA EGADAWFKEG AKERFLSGLV EKPDHWEMVR 600
    DVLDVWFDSG STHAFTLEVR PDLKAARPPE GNDRVMYLEG SDQHRGWFHS 650
    SLLESCGTRG RPPYDVVLTH GFVLDEDGRK MSKSLGNVTS PQDVIKQSGA 700
    DILRLWVCAS DYADDLRIGP EILKTTADTY RKLRNTIRWL LGSLHHDRPA 750
    EHVAFADMPA LERYILHRLV ELDGEIRAAY RAFDYKKVNA ALTQFMNIEL 800
    SAFYFDIRKD ALYCDPLSSV TRRACLTVLD AVFARLITWL APILPFTCEE 850
    AYIARTGDEE GSVHLLGFPD TPADWRDDAL AEKWRQVRVI RRVVLGALEV 900
    ERAAKRMGSS LEAAPEVYVS ESALADALDG LDLAEITITS AATLIRSEGP 950
    AEAFRLPDVP GVAVVPVRAE GRKCARSWKI SAEVGTDPQF PDVTPRDADA 1000
    LREFFAAQTA AQ 1012
    Length:1,012
    Mass (Da):112,627
    Last modified:September 11, 2007 - v1
    Checksum:iF751C59A78001778
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000781 Genomic DNA. Translation: ABS67451.1.
    RefSeqiYP_001417108.1. NC_009720.1.

    Genome annotation databases

    EnsemblBacteriaiABS67451; ABS67451; Xaut_2207.
    GeneIDi5421678.
    KEGGixau:Xaut_2207.
    PATRICi24046503. VBIXanAut29526_2541.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000781 Genomic DNA. Translation: ABS67451.1 .
    RefSeqi YP_001417108.1. NC_009720.1.

    3D structure databases

    ProteinModelPortali A7IHF8.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 78245.Xaut_2207.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS67451 ; ABS67451 ; Xaut_2207 .
    GeneIDi 5421678.
    KEGGi xau:Xaut_2207.
    PATRICi 24046503. VBIXanAut29526_2541.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci XAUT78245:GHS6-2230-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-1158 / Py2Imported.

    Entry informationi

    Entry nameiA7IHF8_XANP2
    AccessioniPrimary (citable) accession number: A7IHF8
    Entry historyi
    Integrated into UniProtKB/TrEMBL: September 11, 2007
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3