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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotationSAAS annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei639Aminoacyl-adenylateUniRule annotation1
Binding sitei683ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetaseUniRule annotationSAAS annotationImported, Ligase
Biological processProtein biosynthesisUniRule annotationSAAS annotation
LigandATP-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Xaut_2207Imported
OrganismiXanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)Imported
Taxonomic identifieri78245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter
Proteomesi
  • UP000002417 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi78245.Xaut_2207

Structurei

3D structure databases

ProteinModelPortaliA7IHF8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 718tRNA-synt_1InterPro annotationAdd BLAST673
Domaini762 – 912Anticodon_1InterPro annotationAdd BLAST151

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi76 – 86"HIGH" regionUniRule annotationAdd BLAST11
Motifi680 – 684"KMSKS" regionUniRule annotation5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 3 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A7IHF8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPDSSAPD TATAEASAAP DYSKTLLLPQ TAFPMRAGLP KLEPELLARW
60 70 80 90 100
ARTDLYGRLR EQGRGRARFV LHDGPPYANG NIHIGHALNK ILKDVVTRSQ
110 120 130 140 150
QMLGYDSNYV PGWDCHGLPI EWKIEEDNYR KKGKTKPDFT DAAAMVAFRQ
160 170 180 190 200
ECRAYAEHWL SEQRKEFKRL GVEGDWDHPY TTMAFAAEAQ IAREIMKFAE
210 220 230 240 250
NGLLYRGSKP VMWSVVEKTA LAEAEVEYQD FTSDTVWVKF PVASEGELKG
260 270 280 290 300
ATVVIWTTTP WTLPGNRAIS YSSKISYGLY EVTEAPADNW AKAGDKLILA
310 320 330 340 350
DKLAEDVFKQ ARVTTFARRG DVPADVLGRL ITAHPLAALG YSFQVPLLDG
360 370 380 390 400
DHVTDDAGTG FVHTAPGHGR EDFEIWTQHR RWLEERGISP AIPYTVDADS
410 420 430 440 450
FYTAQAPGFE GKRVITEKGE KGDANTAVID ALIKAGNLLA RGRVKHQYPH
460 470 480 490 500
SWRSKKPVIF RNTPQWFIAM DQDIRTADGT AAPRPATLAG NAPDTLRERA
510 520 530 540 550
LAGIRTVEWV PAAGENRITG MIANRPDWVV SRQRAWGVPI AVFVKENAYG
560 570 580 590 600
EVVILKDPQV NARIAEAFAA EGADAWFKEG AKERFLSGLV EKPDHWEMVR
610 620 630 640 650
DVLDVWFDSG STHAFTLEVR PDLKAARPPE GNDRVMYLEG SDQHRGWFHS
660 670 680 690 700
SLLESCGTRG RPPYDVVLTH GFVLDEDGRK MSKSLGNVTS PQDVIKQSGA
710 720 730 740 750
DILRLWVCAS DYADDLRIGP EILKTTADTY RKLRNTIRWL LGSLHHDRPA
760 770 780 790 800
EHVAFADMPA LERYILHRLV ELDGEIRAAY RAFDYKKVNA ALTQFMNIEL
810 820 830 840 850
SAFYFDIRKD ALYCDPLSSV TRRACLTVLD AVFARLITWL APILPFTCEE
860 870 880 890 900
AYIARTGDEE GSVHLLGFPD TPADWRDDAL AEKWRQVRVI RRVVLGALEV
910 920 930 940 950
ERAAKRMGSS LEAAPEVYVS ESALADALDG LDLAEITITS AATLIRSEGP
960 970 980 990 1000
AEAFRLPDVP GVAVVPVRAE GRKCARSWKI SAEVGTDPQF PDVTPRDADA
1010
LREFFAAQTA AQ
Length:1,012
Mass (Da):112,627
Last modified:September 11, 2007 - v1
Checksum:iF751C59A78001778
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000781 Genomic DNA Translation: ABS67451.1
RefSeqiWP_012114235.1, NC_009720.1

Genome annotation databases

EnsemblBacteriaiABS67451; ABS67451; Xaut_2207
KEGGixau:Xaut_2207

Similar proteinsi

Entry informationi

Entry nameiA7IHF8_XANP2
AccessioniPrimary (citable) accession number: A7IHF8
Entry historyiIntegrated into UniProtKB/TrEMBL: September 11, 2007
Last sequence update: September 11, 2007
Last modified: March 28, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health