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A7IEC3

- BIOB_XANP2

UniProt

A7IEC3 - BIOB_XANP2

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Protein
Biotin synthase
Gene
bioB, Xaut_1117
Organism
Xanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi76 – 761Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi79 – 791Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi116 – 1161Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi147 – 1471Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi207 – 2071Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi280 – 2801Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciXAUT78245:GHS6-1130-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Xaut_1117
OrganismiXanthobacter autotrophicus (strain ATCC BAA-1158 / Py2)
Taxonomic identifieri78245 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesXanthobacteraceaeXanthobacter
ProteomesiUP000002417: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 345345Biotin synthaseUniRule annotation
PRO_0000381714Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi78245.Xaut_1117.

Structurei

3D structure databases

ProteinModelPortaliA7IEC3.
SMRiA7IEC3. Positions 23-332.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A7IEC3-1 [UniParc]FASTAAdd to Basket

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MTTSTGTTTG TGTARAAEGS LLRSDWTEAE ARALYDLPFP ELMFRAQSVH    50
RRHFDPTTVE TATLLSIKTG GCAEDCGYCS QSAHHETGLK ATKLMGVEEV 100
LSQAQKAKDG GATRFCMGAA WRSPKDRDLD AVCAMISGVK AMGLEACVTL 150
GMLTPDQVTR LGAAGLDYYN HNIDTSPAFY PEIVTTRTMD DRLDTLASVR 200
AGGIRLCTGG IVGMGESVDD RIAMLLVLAT LDPHPESVPI NMWNEIEGTP 250
VMGKADKPDP IAFVRLIALA RLLMPQSIVR LSAGRDAMSD EMQALCFLAG 300
ANSIFTGDVL LTTANPERDK DAALFARLGI RAAAPAATEM RSAAE 345
Length:345
Mass (Da):36,790
Last modified:September 11, 2007 - v1
Checksum:iE4BD311C0E6BCEF7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000781 Genomic DNA. Translation: ABS66366.1.
RefSeqiYP_001416023.1. NC_009720.1.

Genome annotation databases

EnsemblBacteriaiABS66366; ABS66366; Xaut_1117.
GeneIDi5421312.
KEGGixau:Xaut_1117.
PATRICi24044197. VBIXanAut29526_1396.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000781 Genomic DNA. Translation: ABS66366.1 .
RefSeqi YP_001416023.1. NC_009720.1.

3D structure databases

ProteinModelPortali A7IEC3.
SMRi A7IEC3. Positions 23-332.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 78245.Xaut_1117.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS66366 ; ABS66366 ; Xaut_1117 .
GeneIDi 5421312.
KEGGi xau:Xaut_1117.
PATRICi 24044197. VBIXanAut29526_1396.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci XAUT78245:GHS6-1130-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-1158 / Py2.

Entry informationi

Entry nameiBIOB_XANP2
AccessioniPrimary (citable) accession number: A7IEC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: September 11, 2007
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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