A7IB57 (G3P_METB6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase
Short name=GAPDH
EC=1.2.1.59

Alternative name(s):
NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	Mboo_2454

Organism

Methanoregula boonei (strain 6A8) [Complete proteome] [HAMAP]

Taxonomic identifier

456442 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanoregula

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity. HAMAP MF_00559
Subcellular location	Cytoplasm By similarity HAMAP MF_00559.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_1000061116
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	139 – 141	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	194 – 195	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	140	1	Nucleophile By similarity
	Binding site	110	1	NAD; via amide nitrogen By similarity
	Binding site	168	1	NAD By similarity
	Binding site	301	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7IB57 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 5FC38818621545B8	FASTA	343	37,403
	10 20 30 40 50 60 MIKVAINGYG TIGKRVADAV AAQKDMKVIG VSKTRPNAEA FIAKQRGYPL YIADLSKKAA 70 80 90 100 110 120 FEKAGLTVAG SVEDMCKAAD VIVDATPGDI GVTNKPLYEK LGKKALWQGG EDHEVAGFSF 130 140 150 160 170 180 NSSCNFKDAI GRQFIRVVSC NTTGLCRIIN EVDKAFGVEH VHAIMVRRGS DPGEIKKGPI 190 200 210 220 230 240 DAVVLDPVTV PSHHGPDVQT VLPHISITTM AMIVPTTMMH MHAVRITTKK EVNREKVIEL 250 260 270 280 290 300 IKNHPRLGLV KKSAGIKSTA ELKEFAMDLG RQRADLYENC IFEDSIYANK NELCFFQAIH 310 320 330 340 QEADVVVENV DAIRAMTSLA NDGTASIKLT NDALHFVPIQ NNH

References

[1]

"Complete sequence of Candidatus Methanoregula boonei 6A8." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 6A8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000780 Genomic DNA. Translation: ABS56968.1.
RefSeq	YP_001405611.1. NC_009712.1.
3D structure databases
ProteinModelPortal	A7IB57.
SMR	A7IB57. Positions 1-337.
ModBase	Search...
Protein-protein interaction databases
STRING	A7IB57.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5412014.
GenomeReviews	Gene locus Mboo_2454 in contig CP000780_GR.
KEGG	mbn:Mboo_2454.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG04694.
HOGENOM	HBG392099.
OMA	VPSHHGP.
ProtClustDB	PRK04207.
Enzyme and pathway databases
BioCyc	CMET456442:MBOO_2454-MONOMER.
Family and domain databases
HAMAP	MF_00559. G3P_dehdrog_arch. [Tree]
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006436. Glyceraldehyde-3-P_DH_2_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00150.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_METB6

Accession

Primary (citable) accession number: A7IB57

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	September 11, 2007
Last modified:	November 16, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program