A7IAS7 (NADE_METB6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	Mboo_2324

Organism

Methanoregula boonei (strain 6A8) [Complete proteome] [HAMAP]

Taxonomic identifier

456442 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanomicrobia › Methanomicrobiales › Genera incertae sedis › Methanoregula

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000099033
Regions
	Nucleotide binding	31 – 38	8	ATP By similarity
Sites
	Active site	33	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A7IAS7 [UniParc]. Last modified September 11, 2007. Version 1. Checksum: 2F6DAD754CDDC724	FASTA	248	26,893
	10 20 30 40 50 60 MAQELGCRMG QVEQMIRYAY WNSKSQGIVV GVSGGVDSAL AAAFCCRAIG PEKVLGLSLP 70 80 90 100 110 120 ASVSNPQDLS DAQELCAMLG MEHRVVLIDP MLAAFKTIPG FVETPYLLGN LMARIRMTVL 130 140 150 160 170 180 YYHANRDHRL VCGTSNRSEA MLGYCTKYGD NAADFQPIVH LYKTDVYEMA KEVKIPKAIL 190 200 210 220 230 240 EKTPSAGLWA GQSDEGEIGL SYAEIDAALK NLEANGWKAG TPSEEKVLSR AQANAHKRLA APNLLSVP

References

[1]

"Complete sequence of Candidatus Methanoregula boonei 6A8." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Kim E., Zinder S., Richardson P. Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 6A8.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000780 Genomic DNA. Translation: ABS56838.1.
RefSeq	YP_001405481.1. NC_009712.1.
3D structure databases
ProteinModelPortal	A7IAS7.
ModBase	Search...
Protein-protein interaction databases
STRING	A7IAS7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5410519.
GenomeReviews	Gene locus Mboo_2324 in contig CP000780_GR.
KEGG	mbn:Mboo_2324.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	arNOG08797.
HOGENOM	HBG351567.
OMA	YDISARD.
ProtClustDB	CLSK949856.
Enzyme and pathway databases
BioCyc	CMET456442:MBOO_2324-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_METB6

Accession

Primary (citable) accession number: A7IAS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	September 11, 2007
Last modified:	November 16, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program