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A7IAM2

- SYI_METB6

UniProt

A7IAM2 - SYI_METB6

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanoregula boonei (strain 6A8)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMBOO456442:GH2T-2321-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mboo_2269
OrganismiMethanoregula boonei (strain 6A8)
Taxonomic identifieri456442 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanoregula
ProteomesiUP000002408: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10761076Isoleucine--tRNA ligasePRO_1000022151Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi456442.Mboo_2269.

Structurei

3D structure databases

ProteinModelPortaliA7IAM2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionAdd
BLAST
Motifi591 – 5955"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7IAM2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKEVTANFSA KDIEREVQEY WRTHDTYKAV KEQHSAGRAF FFVDGPPYTT
60 70 80 90 100
GQIHLGTAWN KIIKDSILRY HRMNGRNVID RAGYDMHGLP IEVKVEQKLG
110 120 130 140 150
FASKKDIETF GIEKFINECR EFAIKNKELM DAQFENLGIW MDFKNAYQTI
160 170 180 190 200
KPWYVEAAWW TLAKAQEKGM LERGYRVVNW CPRCETAIAD AEVEYWDETD
210 220 230 240 250
PSVFVKFPIR GKVSESLVIW TTTPWTLPAN VAVAVGKEFV YARVHAEKNG
260 270 280 290 300
REEYLWVAED LVKSVLKKGR YQKFETLETK KGAELIGWEY DSPLMDVVPI
310 320 330 340 350
QKEIAHRVVA ADFVAMENTG MVHIAPGHGW DDYVLGTKEK LAIVCPVDGA
360 370 380 390 400
GKFRPETGIF AGKFVRDANG EVLDALGERL LATEKITHRY GHCWRCKTPI
410 420 430 440 450
IFRATSQWFL KASEMRDLML SEVKKVTWYP EWAGSARFYD WIKEARDWCV
460 470 480 490 500
SRQRYWGIPI PVWICDKCDK YRVIGTIAEL EKASGQKVPD PHRPFVDQVT
510 520 530 540 550
IPCECGGTMK RVGDIFDVWF DSAVASWATV GFPAKTDEFE KLWPADFITE
560 570 580 590 600
GQDQTRGWFY SQLGASTIAF GKAPYKSVCM HGFALDAEGR KMSKSLGNVV
610 620 630 640 650
APEEVIAKVG VDVLRLYVLS SSAPWDDLKF NWDGIATVNR TMNILWNVYR
660 670 680 690 700
FPMPYMILDR FEPEAKSGHW DGSFVRSHIR ELPDEDRWIV SRINTVAGIV
710 720 730 740 750
DASTKECQLH RATREILNFI LEDLSRWYVQ LVRPRMWLEG ESEQKIFAYE
760 770 780 790 800
TIYYVMRRLV DLMAPFAPHI TEEIYSNLRC KNDPGSVHML DWQACDDALT
810 820 830 840 850
NRELEHAMEL VRSFDDAVQN ARQAGKRKLR WPVDEVVIVT AKPEVKDAVA
860 870 880 890 900
RLNEVCMDRA NARKVTVVIG RWDRIGWHAE PVMKALGKGF GKNSFAVKGL
910 920 930 940 950
IEAADGNAIK AAVDAGQKFQ LKIEEGKISK PDDLKIGTDY EQDVVEIGIE
960 970 980 990 1000
HVRFTEKLPT DIFSAPMEDG TVYVDVALTP DLEAEGYARE IIRRIQEMRR
1010 1020 1030 1040 1050
QLDLAVEDFI MVDVAVADKR ICELVGASWK PGIADEVRAK TLSLHHSAEP
1060 1070
AGSSHQLAKD WDVEGIAMTI GISKVA
Length:1,076
Mass (Da):122,648
Last modified:September 11, 2007 - v1
Checksum:iFD3F722F961446C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000780 Genomic DNA. Translation: ABS56783.1.
RefSeqiWP_012107843.1. NC_009712.1.
YP_001405426.1. NC_009712.1.

Genome annotation databases

EnsemblBacteriaiABS56783; ABS56783; Mboo_2269.
GeneIDi5411001.
KEGGimbn:Mboo_2269.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000780 Genomic DNA. Translation: ABS56783.1 .
RefSeqi WP_012107843.1. NC_009712.1.
YP_001405426.1. NC_009712.1.

3D structure databases

ProteinModelPortali A7IAM2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 456442.Mboo_2269.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS56783 ; ABS56783 ; Mboo_2269 .
GeneIDi 5411001.
KEGGi mbn:Mboo_2269.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KPVHWCL.

Enzyme and pathway databases

BioCyci MBOO456442:GH2T-2321-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 6A8.

Entry informationi

Entry nameiSYI_METB6
AccessioniPrimary (citable) accession number: A7IAM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 11, 2007
Last modified: November 26, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3