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A7IAM2

- SYI_METB6

UniProt

A7IAM2 - SYI_METB6

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Mboo_2269
Organism
Methanoregula boonei (strain 6A8)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei594 – 5941ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMBOO456442:GH2T-2321-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Mboo_2269
OrganismiMethanoregula boonei (strain 6A8)
Taxonomic identifieri456442 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanoregula
ProteomesiUP000002408: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10761076Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022151Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi456442.Mboo_2269.

Structurei

3D structure databases

ProteinModelPortaliA7IAM2.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi47 – 5711"HIGH" regionUniRule annotation
Add
BLAST
Motifi591 – 5955"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiKPVHWCL.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A7IAM2-1 [UniParc]FASTAAdd to Basket

« Hide

MKEVTANFSA KDIEREVQEY WRTHDTYKAV KEQHSAGRAF FFVDGPPYTT     50
GQIHLGTAWN KIIKDSILRY HRMNGRNVID RAGYDMHGLP IEVKVEQKLG 100
FASKKDIETF GIEKFINECR EFAIKNKELM DAQFENLGIW MDFKNAYQTI 150
KPWYVEAAWW TLAKAQEKGM LERGYRVVNW CPRCETAIAD AEVEYWDETD 200
PSVFVKFPIR GKVSESLVIW TTTPWTLPAN VAVAVGKEFV YARVHAEKNG 250
REEYLWVAED LVKSVLKKGR YQKFETLETK KGAELIGWEY DSPLMDVVPI 300
QKEIAHRVVA ADFVAMENTG MVHIAPGHGW DDYVLGTKEK LAIVCPVDGA 350
GKFRPETGIF AGKFVRDANG EVLDALGERL LATEKITHRY GHCWRCKTPI 400
IFRATSQWFL KASEMRDLML SEVKKVTWYP EWAGSARFYD WIKEARDWCV 450
SRQRYWGIPI PVWICDKCDK YRVIGTIAEL EKASGQKVPD PHRPFVDQVT 500
IPCECGGTMK RVGDIFDVWF DSAVASWATV GFPAKTDEFE KLWPADFITE 550
GQDQTRGWFY SQLGASTIAF GKAPYKSVCM HGFALDAEGR KMSKSLGNVV 600
APEEVIAKVG VDVLRLYVLS SSAPWDDLKF NWDGIATVNR TMNILWNVYR 650
FPMPYMILDR FEPEAKSGHW DGSFVRSHIR ELPDEDRWIV SRINTVAGIV 700
DASTKECQLH RATREILNFI LEDLSRWYVQ LVRPRMWLEG ESEQKIFAYE 750
TIYYVMRRLV DLMAPFAPHI TEEIYSNLRC KNDPGSVHML DWQACDDALT 800
NRELEHAMEL VRSFDDAVQN ARQAGKRKLR WPVDEVVIVT AKPEVKDAVA 850
RLNEVCMDRA NARKVTVVIG RWDRIGWHAE PVMKALGKGF GKNSFAVKGL 900
IEAADGNAIK AAVDAGQKFQ LKIEEGKISK PDDLKIGTDY EQDVVEIGIE 950
HVRFTEKLPT DIFSAPMEDG TVYVDVALTP DLEAEGYARE IIRRIQEMRR 1000
QLDLAVEDFI MVDVAVADKR ICELVGASWK PGIADEVRAK TLSLHHSAEP 1050
AGSSHQLAKD WDVEGIAMTI GISKVA 1076
Length:1,076
Mass (Da):122,648
Last modified:September 11, 2007 - v1
Checksum:iFD3F722F961446C5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000780 Genomic DNA. Translation: ABS56783.1.
RefSeqiWP_012107843.1. NC_009712.1.
YP_001405426.1. NC_009712.1.

Genome annotation databases

EnsemblBacteriaiABS56783; ABS56783; Mboo_2269.
GeneIDi5411001.
KEGGimbn:Mboo_2269.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000780 Genomic DNA. Translation: ABS56783.1 .
RefSeqi WP_012107843.1. NC_009712.1.
YP_001405426.1. NC_009712.1.

3D structure databases

ProteinModelPortali A7IAM2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 456442.Mboo_2269.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABS56783 ; ABS56783 ; Mboo_2269 .
GeneIDi 5411001.
KEGGi mbn:Mboo_2269.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi KPVHWCL.

Enzyme and pathway databases

BioCyci MBOO456442:GH2T-2321-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 6A8.

Entry informationi

Entry nameiSYI_METB6
AccessioniPrimary (citable) accession number: A7IAM2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 11, 2007
Last modified: September 3, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi