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A7IAM2

- SYI_METB6

UniProt

A7IAM2 - SYI_METB6

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanoregula boonei (strain 6A8)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 49 (01 Oct 2014)
      Sequence version 1 (11 Sep 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei594 – 5941ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMBOO456442:GH2T-2321-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Mboo_2269
    OrganismiMethanoregula boonei (strain 6A8)
    Taxonomic identifieri456442 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanoregula
    ProteomesiUP000002408: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10761076Isoleucine--tRNA ligasePRO_1000022151Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi456442.Mboo_2269.

    Structurei

    3D structure databases

    ProteinModelPortaliA7IAM2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi47 – 5711"HIGH" regionAdd
    BLAST
    Motifi591 – 5955"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiKPVHWCL.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A7IAM2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKEVTANFSA KDIEREVQEY WRTHDTYKAV KEQHSAGRAF FFVDGPPYTT     50
    GQIHLGTAWN KIIKDSILRY HRMNGRNVID RAGYDMHGLP IEVKVEQKLG 100
    FASKKDIETF GIEKFINECR EFAIKNKELM DAQFENLGIW MDFKNAYQTI 150
    KPWYVEAAWW TLAKAQEKGM LERGYRVVNW CPRCETAIAD AEVEYWDETD 200
    PSVFVKFPIR GKVSESLVIW TTTPWTLPAN VAVAVGKEFV YARVHAEKNG 250
    REEYLWVAED LVKSVLKKGR YQKFETLETK KGAELIGWEY DSPLMDVVPI 300
    QKEIAHRVVA ADFVAMENTG MVHIAPGHGW DDYVLGTKEK LAIVCPVDGA 350
    GKFRPETGIF AGKFVRDANG EVLDALGERL LATEKITHRY GHCWRCKTPI 400
    IFRATSQWFL KASEMRDLML SEVKKVTWYP EWAGSARFYD WIKEARDWCV 450
    SRQRYWGIPI PVWICDKCDK YRVIGTIAEL EKASGQKVPD PHRPFVDQVT 500
    IPCECGGTMK RVGDIFDVWF DSAVASWATV GFPAKTDEFE KLWPADFITE 550
    GQDQTRGWFY SQLGASTIAF GKAPYKSVCM HGFALDAEGR KMSKSLGNVV 600
    APEEVIAKVG VDVLRLYVLS SSAPWDDLKF NWDGIATVNR TMNILWNVYR 650
    FPMPYMILDR FEPEAKSGHW DGSFVRSHIR ELPDEDRWIV SRINTVAGIV 700
    DASTKECQLH RATREILNFI LEDLSRWYVQ LVRPRMWLEG ESEQKIFAYE 750
    TIYYVMRRLV DLMAPFAPHI TEEIYSNLRC KNDPGSVHML DWQACDDALT 800
    NRELEHAMEL VRSFDDAVQN ARQAGKRKLR WPVDEVVIVT AKPEVKDAVA 850
    RLNEVCMDRA NARKVTVVIG RWDRIGWHAE PVMKALGKGF GKNSFAVKGL 900
    IEAADGNAIK AAVDAGQKFQ LKIEEGKISK PDDLKIGTDY EQDVVEIGIE 950
    HVRFTEKLPT DIFSAPMEDG TVYVDVALTP DLEAEGYARE IIRRIQEMRR 1000
    QLDLAVEDFI MVDVAVADKR ICELVGASWK PGIADEVRAK TLSLHHSAEP 1050
    AGSSHQLAKD WDVEGIAMTI GISKVA 1076
    Length:1,076
    Mass (Da):122,648
    Last modified:September 11, 2007 - v1
    Checksum:iFD3F722F961446C5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000780 Genomic DNA. Translation: ABS56783.1.
    RefSeqiWP_012107843.1. NC_009712.1.
    YP_001405426.1. NC_009712.1.

    Genome annotation databases

    EnsemblBacteriaiABS56783; ABS56783; Mboo_2269.
    GeneIDi5411001.
    KEGGimbn:Mboo_2269.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000780 Genomic DNA. Translation: ABS56783.1 .
    RefSeqi WP_012107843.1. NC_009712.1.
    YP_001405426.1. NC_009712.1.

    3D structure databases

    ProteinModelPortali A7IAM2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 456442.Mboo_2269.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABS56783 ; ABS56783 ; Mboo_2269 .
    GeneIDi 5411001.
    KEGGi mbn:Mboo_2269.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi KPVHWCL.

    Enzyme and pathway databases

    BioCyci MBOO456442:GH2T-2321-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 6A8.

    Entry informationi

    Entry nameiSYI_METB6
    AccessioniPrimary (citable) accession number: A7IAM2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: September 11, 2007
    Last modified: October 1, 2014
    This is version 49 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3