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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Methanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei594ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMBOO456442:G1G9N-2406-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Mboo_2269
OrganismiMethanoregula boonei (strain DSM 21154 / JCM 14090 / 6A8)
Taxonomic identifieri456442 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanomicrobiaMethanomicrobialesMethanoregulaceaeMethanoregula
Proteomesi
  • UP000002408 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000221511 – 1076Isoleucine--tRNA ligaseAdd BLAST1076

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi456442.Mboo_2269

Structurei

3D structure databases

ProteinModelPortaliA7IAM2
SMRiA7IAM2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi47 – 57"HIGH" regionAdd BLAST11
Motifi591 – 595"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00807 Archaea
COG0060 LUCA
HOGENOMiHOG000246403
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG093Z0054

Family and domain databases

CDDicd07961 Anticodon_Ia_Ile_ABEc, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02003 Ile_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033709 Anticodon_Ile_ABEc
IPR002301 Ile-tRNA-ligase
IPR023586 Ile-tRNA-ligase_type2
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A7IAM2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKEVTANFSA KDIEREVQEY WRTHDTYKAV KEQHSAGRAF FFVDGPPYTT
60 70 80 90 100
GQIHLGTAWN KIIKDSILRY HRMNGRNVID RAGYDMHGLP IEVKVEQKLG
110 120 130 140 150
FASKKDIETF GIEKFINECR EFAIKNKELM DAQFENLGIW MDFKNAYQTI
160 170 180 190 200
KPWYVEAAWW TLAKAQEKGM LERGYRVVNW CPRCETAIAD AEVEYWDETD
210 220 230 240 250
PSVFVKFPIR GKVSESLVIW TTTPWTLPAN VAVAVGKEFV YARVHAEKNG
260 270 280 290 300
REEYLWVAED LVKSVLKKGR YQKFETLETK KGAELIGWEY DSPLMDVVPI
310 320 330 340 350
QKEIAHRVVA ADFVAMENTG MVHIAPGHGW DDYVLGTKEK LAIVCPVDGA
360 370 380 390 400
GKFRPETGIF AGKFVRDANG EVLDALGERL LATEKITHRY GHCWRCKTPI
410 420 430 440 450
IFRATSQWFL KASEMRDLML SEVKKVTWYP EWAGSARFYD WIKEARDWCV
460 470 480 490 500
SRQRYWGIPI PVWICDKCDK YRVIGTIAEL EKASGQKVPD PHRPFVDQVT
510 520 530 540 550
IPCECGGTMK RVGDIFDVWF DSAVASWATV GFPAKTDEFE KLWPADFITE
560 570 580 590 600
GQDQTRGWFY SQLGASTIAF GKAPYKSVCM HGFALDAEGR KMSKSLGNVV
610 620 630 640 650
APEEVIAKVG VDVLRLYVLS SSAPWDDLKF NWDGIATVNR TMNILWNVYR
660 670 680 690 700
FPMPYMILDR FEPEAKSGHW DGSFVRSHIR ELPDEDRWIV SRINTVAGIV
710 720 730 740 750
DASTKECQLH RATREILNFI LEDLSRWYVQ LVRPRMWLEG ESEQKIFAYE
760 770 780 790 800
TIYYVMRRLV DLMAPFAPHI TEEIYSNLRC KNDPGSVHML DWQACDDALT
810 820 830 840 850
NRELEHAMEL VRSFDDAVQN ARQAGKRKLR WPVDEVVIVT AKPEVKDAVA
860 870 880 890 900
RLNEVCMDRA NARKVTVVIG RWDRIGWHAE PVMKALGKGF GKNSFAVKGL
910 920 930 940 950
IEAADGNAIK AAVDAGQKFQ LKIEEGKISK PDDLKIGTDY EQDVVEIGIE
960 970 980 990 1000
HVRFTEKLPT DIFSAPMEDG TVYVDVALTP DLEAEGYARE IIRRIQEMRR
1010 1020 1030 1040 1050
QLDLAVEDFI MVDVAVADKR ICELVGASWK PGIADEVRAK TLSLHHSAEP
1060 1070
AGSSHQLAKD WDVEGIAMTI GISKVA
Length:1,076
Mass (Da):122,648
Last modified:September 11, 2007 - v1
Checksum:iFD3F722F961446C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000780 Genomic DNA Translation: ABS56783.1
RefSeqiWP_012107843.1, NC_009712.1

Genome annotation databases

EnsemblBacteriaiABS56783; ABS56783; Mboo_2269
GeneIDi5411001
KEGGimbn:Mboo_2269

Similar proteinsi

Entry informationi

Entry nameiSYI_METB6
AccessioniPrimary (citable) accession number: A7IAM2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: September 11, 2007
Last modified: March 28, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health